In Vitro Evaluation of Biofilm Forming Capacity and Antifungal Resistance of Yeast Isolated from Individuals Aged 18-25 Years

Year 2023, , 1 - 18, 21.07.2023

Gülçin Özcan Ateş , Müşerref Otkun

https://doi.org/10.33484/sinopfbd.1310745

Abstract

Microbiota studies are an increasingly important issue today. In the literature, there are limited studies on the antifungal resistance and biofilm formation capacity of yeasts isolated from the mouths of young individuals. For this reason, our study was carried out with 133 yeast isolates isolated from the mouths of 17 young individuals between the ages of 18-25 in 2018-2020. When the biofilm-forming capacities of 133 isolates were examined, it was determined that 99.25% were biofilm producers by tissue culture plate method and 66.92% by tube method. One hundred thirty-three yeast isolates and seven reference strains were first evaluated against fluconazole antifungal by agar disc diffusion method. The isolates were found to be susceptible to fluconazole. According to this result, 20 isolates with strong biofilm-forming capacity were selected from 133 yeast isolates. Antifungal resistance was evaluated with fluconazole, itraconazole, clotrimazole, amphotericin B and nystatin gradient test strips. It was determined that 20 isolates were resistant to amphotericin B, and 18 were resistant to nystatin antifungal. It was determined that the sensitivity of itraconazole to 14 isolates and clotrimazole to 3 isolates was dose-dependent. As a result, azole group antifungals can be used mainly in treating oral yeast infections.

Keywords

Antifungal resistance, Biofilm, Candida, Gradient test, Oral yeast.

Supporting Institution

Çanakkale Onsekiz Mart üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

THD-2020-3352

Thanks

We would like to thank Prof. Dr. Beyza ENER for the supply of reference culture (Candida albicans ATCC 10231, C. albicans ATCC 14053, C. albicans ATCC 24433, Candida tropicalis ATCC 1021, Candida parapsilosis ATCC 22019, Cryptococcus neoformans ATCC 90112). This study was conducted at Çanakkale Onsekiz Mart University Experimental Research Center (ÇOMÜDAM) and Çanakkale Onsekiz Mart University Faculty of Medicine Medical Microbiology Laboratory.

18-25 Yaş Arası Bireylerden İzole Edilen Mayaların Biyofilm Oluşturma Kapasitesinin ve Antifungal Direncinin İn Vitro Olarak Değerlendirilmesi

Abstract

Mikrobiyota çalışmaları günümüzde önemi giderek artan bir konudur. Literatürde genç bireylerin ağızlarından izole edilen mayaların antifungal direnci ve biyofilm oluşturma kapasitesi üzerine sınırlı sayıda çalışma bulunmaktadır. Bu nedenle çalışma 2018-2020 yıllarında 18-25 yaş arası 17 genç bireyin ağzından izole edilen 133 maya izolatı ile gerçekleştirilmiştir. 133 izolatın biyofilm oluşturma kapasiteleri incelendiğinde doku kültürü plak yöntemi ile %99.25'inin, tüp yöntemi ile %66.92'sinin biyofilm oluşturduğu belirlendi. 133 maya izolatı ve 7 referans suş ilk önce agar disk difüzyon yöntemiyle flukonazol antifungaline karşı değerlendirildi. İzolatların flukonazole duyarlı olduğu bulundu. Bu sonuca göre 133 maya izolatı arasından biyofilm oluşturma kapasitesi güçlü 20 izolat antifungal dirençliliğin belirlenmesi için seçilmiştir. Antifungal direnç flukonazol, itrakonazol, klotrimazol, amfoterisin B ve nistatin gradyan test şeritleri ile değerlendirilmiştir. 20 izolatın amfoterisin B'ye, 18 izolatın ise nistatin antifungaline karşı dirençli olduğu belirlenmiştir. İtrakonazolün 14 izolata ve klotrimazolün 3 izolata duyarlılığının doza bağımlı olduğu tespit edilmiştir. Sonuç olarak, azol grubu antifungaller ağırlıklı olarak oral maya enfeksiyonlarının tedavisinde kullanılabilir.

Keywords

Antifungal direnç, Biyofilm, Candida, Gradiyent test

Project Number

THD-2020-3352

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