Abies nordmanniana subsp. equi-trojani (Aschers. & Sint. ex Boiss) Ekstraktlarının Antibakteriyel ve Antifungal Aktivitesi

Year 2024, , 184 - 193, 29.06.2024

Gülçin Özcan Ateş , Tülay Bican Süerdem

https://doi.org/10.33484/sinopfbd.1404628

Abstract

Bu çalışmada Çanakkale Kazdağlarında yetişen endemik ve yok olma tehlikesiyle karşı karşıya olan Abies nordmanniana subsp. equi-trojani kozalak pullarının antibakteriyel ve antifungal aktivitesi agar kuyu ve mikroplak yöntemiyle araştırılmıştır. Kozalak pullarının distile su, etanol, metanol ve dimetilsülfoksit (DMSO) ekstraktlarının antimikrobiyal aktivitesi gram pozitif ve gram negatif bakteriler ile mayalara karşı değerlendirilmiştir. Sonuç olarak özellikle DMSO ekstraktının test edilen mikroorganizmalara karşı antibakteriyel ve antifungal aktiviteye sahip olduğu tespit edilmiştir. Distile su ile hazırlanan ekstraktının ise herhangi bir aktiviteye sahip olmadığı belirlendi. Metanol ve etanol ekstraktlarının ise mikroorganizma türüne göre aktivite gösterdiği belirlendi. Bu çalışmanın sonuçları pozitif kontrol (penisilin G ve flukonazol) ile karşılaştırıldığında, ekstraktların antibiyotik ve antifungal disk kadar etkili olmadığı belirlendi. Sonuç olarak nesli yok olma tehlikesiyle karşı karşıya kalan bu türün ürünleri yerine doğada bol bulunan alternatif ürünlerin kullanılması önem arz etmektedir.

Keywords

A. equi-trojani, Köknar, Kazdağı, antimikrobiyal aktivite

Thanks

We would like to thank Malatya Kuruyemiş Reis Daşdemir in Çanakkale Küçükkuyu for providing us with Ida Mountain Fir cone spangle and Sevgi KARAKAYA for her help in the supply. We also thank Dr. Nükhet ZORBA for providing us with some standard bacterial cultures.

Antibacterial and Antifungal Activity of Abies nordmanniana subsp. equi-trojani (Aschers. & Sint. ex Boiss) Extracts

Abstract

In this study, the antibacterial and antifungal activity of Abies nordmanniana subsp. equi-trojani cone spangles, which are endemic and endangered and grow in Çanakkale Ida Mountains, was investigated by agar well diffusion and microplate methods. The antibacterial and antifungal activity of distilled water, ethanol, methanol and dimethylsulfoxide (DMSO) extracts of cone spangles was evaluated against gram-positive and gram-negative bacteria and yeasts. As a result, it was determined that the DMSO extract had antibacterial and antifungal activity against the tested microorganisms, while the extract prepared with distilled water had no activity. The antimicrobial activity of the methanol and ethanol extracts varied depending on the microorganism type. When the results of this study were compared with the positive control (penicillin G and fluconazole), it was determined that the extracts were not as effective as the antibiotic and antifungal disc. As a result, it is important to use alternative products that are abundant in nature instead of products derived from endangered species.

Keywords

A. equi-trojani, Fir, Ida Mountains, antimicrobial activity

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