Transferability of barley and wheat EST-microsatellite markers in some Poaceae members

Abstract

The cross species transferability of  barley and wheat microsatellite markers developed from expressed sequence tag (EST) libraries constructed under Fusarium infection conditions were detected among 17 species including 8 from Aegilops, 6 from Triticum, Zea mays, Avena sativa, Oryza sativa.

Transferability rates of barley microsatellite primer pairs ranged from 29% to 100%. A maximum of 100% cross-genera transferability noticed with Avena followed by Zea (92%), Triticum (83%), Aegilops (68%), and Oryza (8%). Primer pairs were highly transferable within species of Triticum (100% in T. turgidum durum durum, 92% in T. turgidum durum dicoccon and T. monococcum aegilopoides, 83% in T.timopheevii timopheevii and T. turgidum dicoccoides, 67% in T. timopheevii armeniacum). Only one primer pairs (contig624) showed 100 % cross-species/genera amplification in all materials studied.

Considering wheat microsatellites, the microsatellite primer pairs were highly transferable within species of Triticum (ranged from %100 to %70) and but low transferable in the allied cereals (15% in Avena, 50% in Oryza, 45% in Zea, 60% in Hordeum). Two primer pairs have shown transferability only in some Triticum species, while two others showed amplication only in species of Aegilops and Triticum. Only one primer pairs showed 100 % cross-species/genera amplification in all materials studied.

This higher cross-species transferability of EST microsatellite markers indicated a high level of conservation of DNA sequences belonging to the transcribed region of the genome and its suitability in comparative genome mapping, genetic diversity and phylogenetics analysis.

Keywords

• Microsatellite markers,transferability

Bazı Poaceae üyelerinde arpa ve buğday EST-mikrosatellit markırlarının aktarılabilirliği

Abstract

Fusariım enfeksiyon koşulları altında oluşturulmuş eksprese olan dizi (EST) kütüphanelerinden geliştirilen arpa ve buğday mikrosatellit markırlarının türler/cinsler arası aktarılabilirlikleri, 8 Aegilops, 6 Triticum türü ile, Zea mays, Avena sativa ve Oryza sativa ‘yı içeren 17 türde belirlenmiştir. Arpa mikrosatellit primerleri için aktarılabilirlik oranı %29 ila %100 arasında değişmiştir. Cinsler arası en yüksek (%100) aktarılabilirlik  Avena da not edilmiş, bunu Zea (%92) , Triticum (%83), Aegilops (68%) ve Oryza (%8) takip etmiştir. Primer çiftleri,  Triticum türleri içerinde oldukça yüksek aktarılabilirdir (T. turgidum durum durum da %100, T. turgidum durum dicoccon ve T. monococcum aegilopoides da %92,T.timopheevii timopheevii ve T. turgidum dicoccoides de %83, T. timopheevii armeniacum da %67). Yalnızca bir primer çifti (contig624) çalışılan tüm materyallerde, %100 tür/cinsler arası çoğaltım göstermiştir.

Buğday mikrosatellitleri değerlendirildiğinde, mikrosatellit primer çiftleri Triticum türleri içinde oldukça aktarılabilirdir (%70 ila %100 arasında) ancak akraba tahıllarda düşük aktarılabilirdir (Avena da 15%, Oryza da 50%, Zea da 45%, Hordeum da 60%). İki primer çifti yalnızca bazı Triticum türlerinde aktarılabilirlik göstermişken, diğer iki primer çifti sadece Aegilops ve Triticum türlerinde çoğaltım göstermiştir.

EST mikrosatellite markırlarının bu yüksek tür/cinsler arası aktarılabilirliği, genomun ifade edilen bölgesine ait DNA diziliminin yüksek derecede korunduğunu ve bunların karşılaştırılmalı genom haritalamalarında, genetik çeşitlilikte ve filogenetik analizlerde uygunluklarını göstermektedir.

Keywords

• mikrosatellit marker,aktarılabilirlik,tahıl

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