Determination of DNA Binding Activities of Naphthalimide Derivatives Containing Side Groups with Different Inductive Effects

Year 2024, Volume: 9 Issue: 1, 145 - 153, 29.06.2024

Ufuk Yıldız , Fatma Özlemiş Melek Ünal Güldan Aydın

https://doi.org/10.33484/sinopfbd.1377778

Abstract

Naphthalimide derivatives appear as compounds with high biological activity in recent studies. In this regard, it is of great importance to determine the DNA interaction pathways of new derivatives and introduce them to literature. In this study, DNA interactions of naphthalimide derivatives containing different side groups were examined. It was understood by UV titration that the binding mode was intercalation. More precise information about the binding mode was obtained by performing competitive fluorescence experiments with ethidium bromide. The form changes of the compounds on plasmid DNA were examined by agarose gel electrophoresis method and the active concentration was decided.

Keywords

DNA, intercalation, naphthalimide

Project Number

1919B012111464

Farklı İndüktif Etkilere Sahip Yan Gruplar İçeren Naftalimit Türevlerinin DNA Bağlanma Aktivitelerinin Belirlenmesi

Abstract

Naftalimit türevleri, son yıllarda yapılan çalışmalarda yüksek biyolojik aktiviteye sahip bileşikler olarak karşımıza çıkmaktadır. Bu doğrultuda yeni türevlerin DNA etkileşim yollarının belirlenmesi ve literatüre kazandırılması büyük önem taşımaktadır. Bu çalışmada farklı yan gruplar içeren naftalimit türevlerinin DNA etkileşimleri incelenmiştir. UV titrasyonu ile bağlanma türünün interkalasyon olduğu anlaşılmıştır. Etidyumbromür ile yarışmalı floresans deneyleri gerçekleştirilerek bağlanma türü hakkında daha kesin bilgiler elde edilmiştir. Bileşiklerin plazmid DNA üzerinde gerçekleştirdikleri form değişiklikleri agaroz jel elektroforez yöntemiyle incelenmiş ve aktif konsantrasyona karar verilmiştir.

Keywords

DNA, interkalasyon, naftalimit

Supporting Institution

TÜBİTAK, Zonguldak Bülent Ecevit Üniversitesi

Project Number

1919B012111464

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