Kronik Stres ve Böbrek: Sıçan Modeli Üzerinde Morfometrik ve Histopatolojik Çalışma

Year 2017, , 168 - 175, 04.01.2018

Elvan Şahin Tuba Demirci , Semin Gedikli

https://doi.org/10.31832/smj.366673

Abstract

Amaç: Günlük yaşamın bir parçası haline gelen stres, vücudun dengesini bozarak
pek çok hastalığa neden olur. Biz de bu çalışmada, kronik strese sebep olan
faktörlere maruz kalındığında böbrekte meydana gelebilecek olası yapısal
değişiklikleri araştırmayı amaçladık.

Gereç ve Yöntem: Bu çalışmada 12 adet Sprague Dawley cinsi yetişkin erkek sıçan
kullanıldı. Sıçanlar rastgele kontrol (n=6) ve stres (n=6) grubu olarak ikiye
ayrıldıktan sonra, stres grubundakilere 4 hafta boyunca “beklenmedik kronik
hafif stres” protokolü uygulandı. Deneyin sonunda anestezisi altında feda
edilen bütün sıçanların böbrekleri çıkarıldı ve derhal “suya daldırma yöntemi”
ile hacimleri ölçüldü. Böbrekler rutin histolojik işlemleri takiben,
histopatolojik değerlendirme için ışık mikroskobunda incelendi.

Bulgular: Stres grubundaki sıçanların böbrek hacimlerinin (0,90 ± 0,096 ml)
kontrol grubundakilerin böbrek hacimlerine (1,075 ± 0,106 ml) kıyasla önemli
düzeyde azalmış olduğu (p<0,05;
independent samples t test)  tespit
edildi. Stres grubu böbreklerinin ışık mikroskobik değerlendirmesinde, nefron
tübüllerinin epitelinde nekrotik dejenerasyon ve dökülmeler saptandı. Proksimal
ve distal tübüllerin epitelinde, yer yer hücresel parçalanma ve tübül duvar
bütünlüğünün bozulması ile sonuçlanan sitoplazmik şişme ve hücresel
vakuolleşmeler belirgindi. Henle kulpunda da lümende bariz dilatasyonlar ve
epitelde nekrotik değişiklikler tespit edildi. İlaveten glomerüllerde büzüşme
ve Bowman boşluğunda genişlemeler gözlendi.

Sonuç: Elde edilen bulgulara dayanarak, kronik stresin böbrekte büyük olasılıkla
fonksiyonel yetersizliğe yol açacak glomerüler hasara ve tübül epitel
hücrelerinde zedelenmeye sebep olduğu düşünüldü.

Keywords

Kronik stres, böbrek, sıçan, histopatoloji, renal hasar, nefron tübülü

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