Renal İskemi-Reperfüzyon Hasarında Antioksidan Olarak Klorojenik Asit (CGA) Kullanımının İncelenmesi: Deneysel Çalışma

Year 2018, , 410 - 415, 29.06.2018

Ayhan Tanyeli , Derya Güzel

https://doi.org/10.31832/smj.420594

Abstract

ÖZET

Amaç: Bu çalışma, sıçanlarda deneysel olarak
oluşturulan böbrek iskemi-reperfüzyon hasarında güçlü antioksidan olan Klorojenik asitin antiinflamatuar ve
antioksidan etkilerini araştırmayı hedeflemektedir.

Gereç ve Yöntemler: Otuz altı adet erişkin,
Wistar albino cinsi dişi sıçan, rastgele dört gruba ayrıldı. Sham grubundaki
sıçanlar (Grup I), sırt bölgesinden açılarak sağ nefrektomi yapıldı.
İskemi-reperfüzyon grubunda (Grup II), sıçanların sırt bölgesi açılıp sağ nefrektomi
yapıldıktan sonra sol renal arter klemplendi. Grup III ‘te 5 mg/kg Klorojenik
asit iskemiden 10 dk önce ve reperfüzyondan 10 dk önce olmak üzere iki doz
şeklinde intraperitoneal olarak uygulandı. Sol böbreğe 1 saatlik iskemiden
sonra 24 saatlik reperfüzyon uygulandı. Grup IV’te Grup III’ten farklı olarak
10 mg/kg Klorojenik asit  uygulandı.
Böbrek dokusunda total antioksidan düzeyi (TAS), total oksidan düzeyi (TOS),
süperoksit dismutaz (SOD), malondialdehit (MDA) ve myeloperoksidaz (MPO)
seviyeleri spektrofotometrik yöntemlerle ölçüldü. Oksidatif stres indeksi (OSI)
hesaplandı.

Bulgular: Grup II’de Grup I’e kıyasla TOS, MDA ve MPO
düzeylerinin arttığı; TAS ve SOD düzeylerinin
ise azaldığı görüldü (her bir parametre için p<0.001). Klorojenik
asit uygulanan gruplarda böbrek TOS (p<0.001), MDA (p<0.001)
ve MPO (p<0.001) düzeylerinde azalma;
TAS (p<0.05) ve SOD (p<0.001)
değerlerinde ise artış tespit edildi.

Sonuç: Böbrek
İ/R hasarında oksidatif stresin önemli rol oynadığı ve bu hasara karşı
Klorojenik asit   uygulamasının oksidatif
hasarı belirgin olarak azalttığı görüldü.

Keywords

Klorojenik asit, renal iskemi reperfüzyon, oksidatif hasar

References

• 1. Fadillioglu E, Kurcer Z, Parlakpinar H, Iraz M, Gursul C. Melatonin treatment against remote organ injury induced by renal ischemia reperfusion injury in diabetes mellitus. Archives of pharmacal research, 2008, 31: 705-712.2. Bonventre JV, Zuk A. Ischemic acute renal failure: an inflammatory disease? Kidney international, 2004, 66: 480-485.3. Yun Y, Duan W, Chen P, Wu H, Shen Z, Qian Z, Wang D In Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats, Transplantation proceedings, (editör).^(editörler). Elsevier: 2009; 3597-3602.4. Linas SL, Shanley PF, Whittenburg D, Berger E, Repine JE. Neutrophils accentuate ischemia-reperfusion injury in isolated perfused rat kidneys. American Journal of Physiology-Renal Physiology, 1988, 255: F728-F735.5. Lin Y, Sheng M, Ding Y, Zhang N, Song Y, Du H, Lu N, Yu W. Berberine protects renal tubular cells against hypoxia/reoxygenation injury via the Sirt1/p53 pathway. Journal of Natural Medicines: 1-9.6. Vogelaar PC, Roorda M, de Vrij EL, Houwertjes MC, Goris M, Bouma H, van der Graaf AC, Krenning G, Henning RH. The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats. Nephrology Dialysis Transplantation, 2018.7. de Sotillo DVR, Hadley M. Chlorogenic acid modifies plasma and liver concentrations of: cholesterol, triacylglycerol, and minerals in (fa/fa) Zucker rats. The Journal of nutritional biochemistry, 2002, 13: 717-726.8. Chang W-C, Chen C-H, Lee M-F, Chang T, Yu Y-M. Chlorogenic acid attenuates adhesion molecules upregulation in IL-1β-treated endothelial cells. European journal of nutrition, 2010, 49: 267-275.9. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 1979, 95: 351-358.10. Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 2005, 38: 1103-1111.11. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 1988, 34: 497-500.12. Khalil AA, Aziz FA, Hall JC. Reperfusion injury. Plastic and reconstructive surgery, 2006, 117: 1024-1033.13. Ames BN. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases. Science, 1983, 221: 1256-1264.14. Avci E, Akarslan Z, Erten H, Coskun-Cevher S. Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers. Brazilian Journal of Medical and Biological Research, 2014, 47: 355-360.15. Kasai H, Fukada S, Yamaizumi Z, Sugie S, Mori H. Action of chlorogenic acid in vegetables and fruits as an inhibitor of 8-hydroxydeoxyguanosine formation in vitro and in a rat carcinogenesis model. Food and Chemical Toxicology, 2000, 38: 467-471.16. Sondheimer E. Chlorogenic acids and related depsides. The Botanical Review, 1964, 30: 667-712.17. Cinkilic N, Cetintas SK, Zorlu T, Vatan O, Yilmaz D, Cavas T, Tunc S, Ozkan L, Bilaloglu R. Radioprotection by two phenolic compounds: chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro. Food and Chemical Toxicology, 2013, 53: 359-363.18. Oboh G, Agunloye OM, Akinyemi AJ, Ademiluyi AO, Adefegha SA. Comparative study on the inhibitory effect of caffeic and chlorogenic acids on key enzymes linked to Alzheimer’s disease and some pro-oxidant induced oxidative stress in rats’ brain-in vitro. Neurochemical research, 2013, 38: 413-419.19. Dos Santos MD, Almeida MC, Lopes NP, De Souza GEP. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biological and Pharmaceutical Bulletin, 2006, 29: 2236-2240.20. Tsai KL, Hung CH, Chan SH, Hsieh PL, Ou HC, Cheng YH, Chu PM. Chlorogenic Acid Protects Against oxLDL‐Induced Oxidative Damage and Mitochondrial Dysfunction by Modulating SIRT1 in Endothelial Cells. Molecular nutrition & food research, 2018: 1700928.21. Shi H, Shi A, Dong L, Lu X, Wang Y, Zhao J, Dai F, Guo X. Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress. Clinical Nutrition, 2016, 35: 1366-1373.