Protective Effect of p-Coumaric Acid as Free Oxygen Radical Scavenger in Experimental Renal Ischemia-Reperfusion Model

Year 2018, , 625 - 631, 30.09.2018

Derya Güzel , Ayhan Tanyeli

https://doi.org/10.31832/smj.455724

Cited By: 1

Abstract

 

ÖZET

Amaç: Bu
çalışmanın amacı, sıçan böbrek dokusunda iskemi-reperfüzyona bağlı doku hasarı
üzerine p-kumarik asit (p-CA)’in antioksidan etkisini değerlendirmektir.

Gereç ve Yöntemler: 12-16 haftalık 200-250 gr otuz
iki adet Wistar Albino cinsi dişi sıçan temin edildi. Sıçanlara intraperitonal olarak
ketamine (75 mg/kg) ve xylazine (8 mg/kg) anestezisi altında sırt bölgesinden
yapılan bir kesi ile sağ nefrektomi uygulandı. Bu sıçanlar randomize olarak
dört gruba eşit olarak bölündü (n=8). Gruplar; sham (S), renal iskemi-reperfüzyon
(R-IR), R-IR ile 50 mg/kg p-kumarik asit (p-CA 50) ve R-IR ile 100 mg/kg p-kumarik
asit (p-CA 100) uygulanan grup olarak sınıflandırıldı. Sham hariç diğer
gruplardaki sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan sonra sol
renal arter klemplenerek  iskemi ve
reperfüzyon protokolü uygulandı. P-kumarik asit iskemiden 60 dk önce ve
reperfüzyon başlangıcından 30 dk önce olmak üzere iki doz şeklinde uygulandı.
İşlemler tamamlandıktan sonra sol böbrek total antioksidan düzeyi (TAS), total
oksidan düzeyi (TOS), süperoksit dismutaz (SOD), malondialdehit (MDA) ve
myeloperoksidaz (MPO) seviyeleri spektrofotometrik yöntemlerle ölçüldü.
Oksidatif stres indeksi (OSI) hesaplandı.

Bulgular: R-IR grubunda S ile karşılaştırıldığında TAS
ve SOD değeri düşerken, TOS, MDA, MPO ve OSI değerleri yükseldi (p<0.01). P-CA
uygulanan gruplarda TAS ve SOD değerinde yükselme, TOS, MDA ve MPO değerlerinde
ise düşme gözlendi. (p<0.05).

Sonuç: Bulgularımız, renal iskemi
reperfüzyon hasarında p-CA uygulamasının oksidan hasarı azaltarak dokunun
korunmasında rol oynayabileceğini göstermektedir.  

Keywords

p-coumaric acid, renal ischemia-reperfusion injury, oxidative stress.

References

• 1. Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. J Renal Inj Prev, 2015, 4: 20-7.2. Rovcanin B, Medic B, Kocic G, Cebovic T, Ristic M, Prostran M. Molecular Dissection of Renal Ischemia-Reperfusion: Oxidative Stress and Cellular Events. Curr Med Chem, 2016, 23: 1965-80.3. Xue L, Xie K, Han X, Yang Z, Qiu J, Zhao Z, Bao T. Detrimental functions of IL-17A in renal ischemia-reperfusion injury in mice. J Surg Res, 2011, 171: 266-74.4. Eltzschig HK, Eckle T. Ischemia and reperfusion--from mechanism to translation. Nat Med, 2011, 17: 1391-401.5. Lameire NH, Bagga A, Cruz D, De Maeseneer J, Endre Z, Kellum JA, Liu KD, Mehta RL, Pannu N, Van Biesen W, Vanholder R. Acute kidney injury: an increasing global concern. Lancet, 2013, 382: 170-9.6. Tas Hekimoglu A, Toprak G, Akkoc H, Evliyaoglu O, Ozekinci S, Kelle I. Oxytocin ameliorates remote liver injury induced by renal ischemia-reperfusion in rats. Korean J Physiol Pharmacol, 2013, 17: 169-73.7. Chawla LS, Kimmel PL. Acute kidney injury and chronic kidney disease: an integrated clinical syndrome. Kidney Int, 2012, 82: 516-24.8. Jang HR, Ko GJ, Wasowska BA, Rabb H. The interaction between ischemia-reperfusion and immune responses in the kidney. J Mol Med (Berl), 2009, 87: 859-64.9. Stroo I, Stokman G, Teske GJ, Raven A, Butter LM, Florquin S, Leemans JC. Chemokine expression in renal ischemia/reperfusion injury is most profound during the reparative phase. Int Immunol, 2010, 22: 433-42.10. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol, 2000, 190: 255-66.11. Lee DM, Hoffman WH, Carl GF, Khichi M, Cornwell PE. Lipid peroxidation and antioxidant vitamins prior to, during, and after correction of diabetic ketoacidosis. J Diabetes Complications, 2002, 16: 294-300.12. Vaghasiya JD, Sheth NR, Bhalodia YS, Jivani NP. Exaggerated liver injury induced by renal ischemia reperfusion in diabetes: effect of exenatide. Saudi J Gastroenterol, 2010, 16: 174-80.13. Jang HS, Han JH, Jeong JY, Sohn UD. Protective Effect of ECQ on Rat Reflux Esophagitis Model. Korean J Physiol Pharmacol, 2012, 16: 455-62.14. Jung J, Nam Y, Sohn UD. Inhibitory Effects of ECQ on Indomethacin-Induced Gastric Damage in Rats. Korean J Physiol Pharmacol, 2012, 16: 399-404.15. Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res, 1998, 39: 1529-42.16. Lavelli V, Peri C, Rizzolo A. Antioxidant activity of tomato products as studied by model reactions using xanthine oxidase, myeloperoxidase, and copper-induced lipid peroxidation. J Agric Food Chem, 2000, 48: 1442-8.17. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol, 1982, 78: 206-9.18. Zhao Z, Gong S, Wang S, Ma C. Effect and mechanism of evodiamine against ethanol-induced gastric ulcer in mice by suppressing Rho/NF-small ka, CyrillicB pathway. Int Immunopharmacol, 2015, 28: 588-95.19. El-Seedi HR, El-Said AM, Khalifa SA, Goransson U, Bohlin L, Borg-Karlson AK, Verpoorte R. Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids. J Agric Food Chem, 2012, 60: 10877-95.20. Yoon SA, Kang SI, Shin HS, Kang SW, Kim JH, Ko HC, Kim SJ. p-Coumaric acid modulates glucose and lipid metabolism via AMP-activated protein kinase in L6 skeletal muscle cells. Biochem Biophys Res Commun, 2013, 432: 553-7.21. Kilic I, Yesiloglu Y. Spectroscopic studies on the antioxidant activity of p-coumaric acid. Spectrochim Acta A Mol Biomol Spectrosc, 2013, 115: 719-24.22. Kadoma Y, Fujisawa S. A comparative study of the radical-scavenging activity of the phenolcarboxylic acids caffeic acid, p-coumaric acid, chlorogenic acid and ferulic acid, with or without 2-mercaptoethanol, a thiol, using the induction period method. Molecules, 2008, 13: 2488-99.23. Yue Y, Shen P, Xu Y, Park Y. p-Coumaric acid improves oxidative and osmosis stress responses in Caenorhabditis elegans. J Sci Food Agric, 2018.24. Navaneethan D, Rasool MK. An experimental study to investigate the impact of p-coumaric acid, a common dietary polyphenol, on cadmium chloride-induced renal toxicity. Food Funct, 2014, 5: 2438-45.25. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 1979, 95: 351-358.26. Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 2005, 38: 1103-1111.27. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 1988, 34: 497-500.28. Kulshreshtha R, Ferracin M, Wojcik SE, Garzon R, Alder H, Agosto-Perez FJ, Davuluri R, Liu CG, Croce CM, Negrini M, Calin GA, Ivan M. A microRNA signature of hypoxia. Mol Cell Biol, 2007, 27: 1859-67.29. Kulshreshtha R, Davuluri RV, Calin GA, Ivan M. A microRNA component of the hypoxic response. Cell Death Differ, 2008, 15: 667-71.30. Jansen EH, Ruskovska T. Comparative Analysis of Serum (Anti)oxidative Status Parsmall a, Cyrillicmeters in Healthy Persons. Int J Mol Sci, 2013, 14: 6106-15.31. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 2005, 38: 1103-11.32. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 2004, 37: 277-85.33. Harma M, Harma M, Erel O. Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly, 2003, 133: 563-6.34. Davies GR, Simmonds NJ, Stevens TR, Grandison A, Blake DR, Rampton DS. Mucosal reactive oxygen metabolite production in duodenal ulcer disease. Gut, 1992, 33: 1467-72.35. Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 1995, 41: 1819-28.36. Abbas AM, Sakr HF. Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats. J Physiol Biochem, 2013, 69: 527-37.37. Rangan U, Bulkley GB. Prospects for treatment of free radical-mediated tissue injury. Br Med Bull, 1993, 49: 700-18.38. Liu YH, Zhang ZB, Zheng YF, Chen HM, Yu XT, Chen XY, Zhang X, Xie JH, Su ZQ, Feng XX, Zeng HF, Su ZR. Gastroprotective effect of andrographolide sodium bisulfite against indomethacin-induced gastric ulceration in rats. Int Immunopharmacol, 2015, 26: 384-91.39. Scalbert A, Williamson G. Dietary intake and bioavailability of polyphenols. J Nutr, 2000, 130: 2073s-85s.40. King A, Young G. Characteristics and occurrence of phenolic phytochemicals. J Am Diet Assoc, 1999, 99: 213-8.41. Ursini F, Tubaro F, Rong J, Sevanian A. Optimization of nutrition: polyphenols and vascular protection. Nutr Rev, 1999, 57: 241-9.42. Dixon RA. Natural products and plant disease resistance. Nature, 2001, 411: 843-7.43. Abdel-Wahab MH, El-Mahdy MA, Abd-Ellah MF, Helal GK, Khalifa F, Hamada FM. Influence of p-coumaric acid on doxorubicin-induced oxidative stress in rat's heart. Pharmacol Res, 2003, 48: 461-5.44. Amalan V, Vijayakumar N, Indumathi D, Ramakrishnan A. Antidiabetic and antihyperlipidemic activity of p-coumaric acid in diabetic rats, role of pancreatic GLUT 2: In vivo approach. Biomed Pharmacother, 2016, 84: 230-236.45. Guven M, Aras AB, Akman T, Sen HM, Ozkan A, Salis O, Sehitoglu I, Kalkan Y, Silan C, Deniz M, Cosar M. Neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia. Iran J Basic Med Sci, 2015, 18: 356-63.46. Mestrovic J, Pogorelic Z, Drmic-Hofman I, Vilovic K, Todoric D, Popovic M. Protective effect of urapidil on testicular torsion-detorsion injury in rats. Surg Today, 2017, 47: 393-398.47. Stanely Mainzen Prince P, Roy AJ. p-Coumaric acid attenuates apoptosis in isoproterenol-induced myocardial infarcted rats by inhibiting oxidative stress. Int J Cardiol, 2013, 168: 3259-66.48. Koyuncu I, Gonel A, Akdag A, Yilmaz MA. Identification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L. Cell Mol Biol (Noisy-le-grand), 2018, 64: 75-83.49. Gulcin I. Antioxidant and antiradical activities of L-carnitine. Life Sci, 2006, 78: 803-11.50. Buyukokuroglu ME, GulcIn I, Oktay M, Kufrevioglu OI. In vitro antioxidant properties of dantrolene sodium. Pharmacol Res, 2001, 44: 491-4.51. Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. J Ethnopharmacol, 2003, 86: 51-8.52. Lee S, Kim HB, Hwang ES, Kim ES, Kim SS, Jeon TD, Song MC, Lee JS, Chung MC, Maeng S, Park JH. Antidepressant-like Effects of p-Coumaric Acid on LPS-induced Depressive and Inflammatory Changes in Rats. Exp Neurobiol, 2018, 27: 189-199.53. Song A, Ko HJ, Lai MN, Ng LT. Protective effects of Wu-Ling-Shen (Xylaria nigripes) on carbon tetrachloride-induced hepatotoxicity in mice. Immunopharmacol Immunotoxicol, 2011, 33: 454-60.54. Yeh CT, Ching LC, Yen GC. Inducing gene expression of cardiac antioxidant enzymes by dietary phenolic acids in rats. J Nutr Biochem, 2009, 20: 163-71.55. Maruf AA, Lip H, Wong H, O'Brien PJ. Protective effects of ferulic acid and related polyphenols against glyoxal- or methylglyoxal-induced cytotoxicity and oxidative stress in isolated rat hepatocytes. Chem Biol Interact, 2015, 234: 96-104.56. Lee SJ, Mun GI, An SM, Boo YC. Evidence for the association of peroxidases with the antioxidant effect of p-coumaric acid in endothelial cells exposed to high glucose plus arachidonic acid. BMB Rep, 2009, 42: 561-7.57. Kim W, Lim D, Kim J. p-Coumaric Acid, a Major Active Compound of Bambusae Caulis in Taeniam, Suppresses Cigarette Smoke-Induced Pulmonary Inflammation. Am J Chin Med, 2018, 46: 407-421.58. Neog MK, Joshua Pragasam S, Krishnan M, Rasool M. p-Coumaric acid, a dietary polyphenol ameliorates inflammation and curtails cartilage and bone erosion in the rheumatoid arthritis rat model. Biofactors, 2017, 43: 698-717.59. Abdel-Moneim A, Yousef AI, Abd El-Twab SM, Abdel Reheim ES, Ashour MB. Gallic acid and p-coumaric acid attenuate type 2 diabetes-induced neurodegeneration in rats. Metab Brain Dis, 2017, 32: 1279-1286.60. Pragasam SJ, Rasool M. Dietary component p-coumaric acid suppresses monosodium urate crystal-induced inflammation in rats. Inflamm Res, 2013, 62: 489-98.61. Pragasam SJ, Venkatesan V, Rasool M. Immunomodulatory and anti-inflammatory effect of p-coumaric acid, a common dietary polyphenol on experimental inflammation in rats. Inflammation, 2013, 36: 169-76.