Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats

Abstract

Objective: The aim of this study is to prove whether or not the cannabinoid (CB)₂ receptor agonist, which is known to be effective in anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion (I/R) injuries, works in the treatment of I/R injury, which is a replication of the damage caused by clinical ovarian torsion.

Materials and Methods: Rats were divided into five groups (n=6). After anesthesia, an approximately 2-cm incision was made in the lower abdominal region of the rats and both ovaries, uterine horns, and adnexa were occluded with a clamp for 30 min, except for the sham control group. After 3 h, both ovaries were surgically removed. Blood was collected for the measurement of cytokine levels in the serum.

Results: The induction of ovaries by I/R injury resulted in an increase in interleukin 1 beta, tumor necrosis factor alpha, and malondialdehyde levels and decrease in glutathione (GSH) levels in ovarian tissues and serum. The pretreatment of the CB₂ receptor agonist decreased these cytokines levels in both ovarian tissues and serum. The GSH level in both was reduced by I/R injury, while the agonist increased the GSH level reduced by I/R injury. The administration of the antagonist reversed the curative effect of the agonist.

Conclusion: The results of this study support that the CB₂ receptor agonist can be used to treat I/R injury, which is an imitation of ovarian torsion. However, no the clinical trial is a limitation of this study.

Keywords

• I/R,AM1241,ovarian torsion,cytokines,AM630

Supporting Institution

Adiyaman University

Project Number

TIPFYL/2019-0001

Kannabinoid Tip 2 Reseptör Agonistinin Sıçanlarda Over İskemi/reperfüzyon Hasarına Karşı Koruyucu Etkisi

Abstract

Amaç: Bu çalışmanın amacı, anti-enflamatuar etkili olduğu bilinen ve çeşitli organ ve / veya doku iskemisi / reperfüzyon (I / R) yaralanmalarında denenen kannabinoid (CB) 2 reseptör agonistinin klinik over torsiyonunun neden olduğu hasarın bir kopyası olan I / R hasarının tedavisinde etkisinin olup olmadığını kanıtlamaktır.

Gereç ve yöntemler: Ratlar beş gruba ayrıldı (n = 6). Anestezi sonrası, sıçanların alt karın bölgesinde yaklaşık 2 cm'lik bir insizyon yapıldı ve her iki yumurtalık, rahim boynuzu ve adneks, şam kontrol grubu hariç 30 dakika boyunca bir klemp ile kapatıldı. 3 saat sonra, her iki yumurtalık cerrahi olarak çıkarıldı. Serumdaki sitokin seviyelerinin ölçümü için kan alındı.

Bulgular: Yumurtalıkların I / R hasarı ile indüklenmesi, interlökin 1 beta (IL-1β), tümör nekroz faktörü alfa (TNF-α) ve malondialdehit (MDA) seviyelerinde bir artışa ve yumurtalık dokularında ve serumda glutatyon (GSH) seviyelerinde bir azalmaya neden olmuştur. CB2 reseptörü agonistinin ön tedavisi, hem over dokularında hem de serumda bu sitokin seviyelerini düşürmüştür. Her ikisindeki GSH seviyesi I / R hasarı ile azalırken, agonist I / R hasarı tarafından azaltılmış GSH seviyesini arttırmıştır. Antagonistin verilmesi, agonistin iyileştirici etkisini tersine çevirdi.

Sonuç: Bu çalışmanın sonuçları CB2 reseptörü agonistinin, yumurtalık torsiyonunun bir taklidi olan I / R hasarını tedavi etmek için kullanılabileceğini desteklemektedir. Ancak, klinik çalışma yapılmaması bu çalışmanın bir sınırlamasıdır.

Keywords

• I/R,AM1241,ovaryum torsiyonu,sitokinler,AM630

Project Number

TIPFYL/2019-0001

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