Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats

Year 2019, , 679 - 686, 16.12.2019

Buket Bilgin Şahin Ali Parlar

https://doi.org/10.31832/smj.626204

Abstract

Objective: The aim of this study is to prove whether or not the cannabinoid
(CB)₂ receptor agonist, which is known to be effective in
anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion
(I/R) injuries, works in the treatment of I/R injury, which is a replication of
the damage caused by clinical ovarian torsion.

Materials
and Methods: Rats were divided
into five groups (n=6). After anesthesia, an approximately 2-cm incision was
made in the lower abdominal region of the rats and both ovaries, uterine horns,
and adnexa were occluded with a clamp for 30 min, except for the sham control group.
After 3 h, both ovaries were surgically removed. Blood was collected for the
measurement of cytokine levels in the serum.

Results:
The induction of ovaries by
I/R injury resulted in an increase in interleukin 1 beta, tumor necrosis factor
alpha, and malondialdehyde levels and decrease in glutathione (GSH) levels in
ovarian tissues and serum. The pretreatment of the CB₂
receptor agonist decreased these cytokines levels in both ovarian tissues and
serum. The GSH level in both was reduced by I/R injury, while the agonist
increased the GSH level reduced by I/R injury. The administration of the
antagonist reversed the curative effect of the agonist.

Conclusion: The results of this study support that the CB₂
receptor agonist can be used to treat I/R injury, which is an imitation of
ovarian torsion. However, no the clinical trial is a limitation of this study.

Keywords

I/R, AM1241, ovarian torsion, cytokines, AM630

Supporting Institution

Adiyaman University

Project Number

TIPFYL/2019-0001

Kannabinoid Tip 2 Reseptör Agonistinin Sıçanlarda Over İskemi/reperfüzyon Hasarına Karşı Koruyucu Etkisi

Abstract

Amaç: Bu çalışmanın amacı, anti-enflamatuar etkili olduğu
bilinen ve çeşitli organ ve / veya doku iskemisi / reperfüzyon (I / R)
yaralanmalarında denenen kannabinoid (CB) 2 reseptör agonistinin klinik over
torsiyonunun neden olduğu hasarın bir kopyası olan I / R hasarının tedavisinde
etkisinin olup olmadığını kanıtlamaktır.

Gereç
ve yöntemler: Ratlar
beş gruba ayrıldı (n = 6). Anestezi sonrası, sıçanların alt karın bölgesinde
yaklaşık 2 cm'lik bir insizyon yapıldı ve her iki yumurtalık, rahim boynuzu ve
adneks, şam kontrol grubu hariç 30 dakika boyunca bir klemp ile kapatıldı. 3
saat sonra, her iki yumurtalık cerrahi olarak çıkarıldı. Serumdaki sitokin
seviyelerinin ölçümü için kan alındı.

Bulgular: Yumurtalıkların I / R hasarı ile indüklenmesi,
interlökin 1 beta (IL-1β), tümör nekroz faktörü alfa (TNF-α) ve malondialdehit
(MDA) seviyelerinde bir artışa ve yumurtalık dokularında ve serumda glutatyon
(GSH) seviyelerinde bir azalmaya neden olmuştur. CB2 reseptörü agonistinin ön
tedavisi, hem over dokularında hem de serumda bu sitokin seviyelerini
düşürmüştür. Her ikisindeki GSH seviyesi I / R hasarı ile azalırken, agonist I
/ R hasarı tarafından azaltılmış GSH seviyesini arttırmıştır. Antagonistin
verilmesi, agonistin iyileştirici etkisini tersine çevirdi.

Sonuç: Bu çalışmanın sonuçları CB2 reseptörü agonistinin,
yumurtalık torsiyonunun bir taklidi olan I / R hasarını tedavi etmek için
kullanılabileceğini desteklemektedir. Ancak, klinik çalışma yapılmaması bu
çalışmanın bir sınırlamasıdır.

Keywords

I/R, AM1241, ovaryum torsiyonu, sitokinler, AM630

Project Number

TIPFYL/2019-0001

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