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Sıçanlarda Kalori Kısıtlamasının Lipid Peroksidasyonu Ve Antioksidan Enzimlere Etkisi

Year 2013, Volume: 3 Issue: 1, 19 - 24, 01.03.2013
https://doi.org/10.5505/sakaryamj.2013.98700

Abstract

Amaç: İnsanlarda ve diğer canlı türlerinde vücut homeostazını bozmadan, esansiyel besin gereksinimi sürdürülürken alınan kalorinin azaltılmasına kalori kısıtlaması denir. Kalori kısıtlanmasının, obeziteyi önlemenin yanısıra yaşlanmanın gecikmesi ve yaşlanmaya bağlı çeşitli patolojilerin önlenmesi gibi etkilerinin olduğu görülmüştür. Bu çalışmada 10 hafta süreyle alınan kalorinin %60 oranında azaltılması şeklinde kalori kısıtlaması uygulanmış ve diet uygulamasının oksidan ve antioksidan belirteçler üzerine etkisi araştırılmıştır.Gereç ve Yöntem: Wistar Albino cinsi, 40 adet, 4-5 aylık, erkek sıçan kullanılmıştır. Normal kilolu kontrol (NK, n=10), normal kilolu kalori kısıtlaması yapılan (ND, n=10), obez kontrol (OK, n=10), obez kalori kısıtlaması yapılan (OD, n=10) gruplar şeklinde 4 grup oluşturulmuştur. Deney başlangıcında normal kilolu grup (NK) 250±20 gr, obez grup (OK) 400±20 gr idi. 10. haftanın sonunda, anestezi altında aorttan kan alınarak deney sonlandırılmıştır. Numunelerden hemolizat hazırlanarak lipid peroksidasyon ürünü MDA, antioksidan enzimler; SOD, KAT ve GSH çalışılmıştır. Bulgular: OD grubu MDA düzeyi, OK grubuna göre anlamlı düzeyde düşük iken, SOD, CAT ve GSH düzeyleri OD grubunda OK grubuna göre anlamlı düzeyde yüksektir (P

References

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  • Çağlayan F, Çağlayan O, Günel E, Çakmak M. The effect of fasting on the oxidant stres, J Med Sci 2001; 21:374-376.
  • Mannarino SC, Amorim MA, Pereira MD, Moradas-Ferreira P, Panek AD, Costa V, Eleutherio EC. Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae. Mech Ageing Dev. 2008; 129(12):700- 5.
  • Gong X, Shang F, Obin M, Palmer H, Scrofano MM, Jahngen- Hodge J, Smith DE, Taylor A. Antioxidant enzyme activities in lens, liver and kidney of calorie restricted Emory mice. Mech Ageing Dev. 1997; 99(3):181-92.
  • Masoro EJ, Iwaski K, Gleiser CA, McMahan CA, Seo E, Yu BP. Dietary modulation of the progression of nephropathy in aging rats: an evaluation of the importance of protein, Am. J. Clin. Nutr. 1989; 49:1217–1227 Scopus.
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  • Percy CJ, Brown L, Power DA, Johnson DW, Gobe GC. Obesity and hypertension have differing oxidant handling molecular pathways in age-related chronic kidney disease. Mech Ageing Dev. 2009; 130(3):129-38.
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  • Gülden Burçak, Gülnur Andican. Oksidatif DNA Hasarı ve yaşlanma. (Oxidative DNA damage and aging) Cerrahpaşa J Med 2004; 35:159-169.
  • Silver AE, Bekse SD, Christou DD, Donato AJ, Moreau KL, Eskurza I, Gates PE and Seals DR. 2007. Overweight and Obese Humans Demonstrate Increased Vascular Endothelial NAD(P) H Oxidase-p47 phox Expression and Evidence of Endothelial Oxidative Stres. Circulation. 2007; 115:627-637.
  • Masoro EJ. Caloric restriction-induced life extension of rats and mice: a critique of proposed mechanisms. Biochim Biophys Acta. 2009; 1790(10):1040-8.
  • Ugochukwu NH, Mukes JD, Figgers CL. Ameliorative effects of dietary caloric restriction on oxidative stress and inflammation in the brain of streptozotocin-induced diabetic rats. Clin Chim Acta. 2006; 370(1-2):165-73.
  • Yang MH, Schaich KM. Factors affecting DNA damage caused by lipid hydroperoxides and aldehydes. Free Radic Biol Med. 1996; 20(2):225-36.
  • Sohal RS, Weindruch R. Oxidative stress, caloric restriction, and aging. Science 1996; 5;273(5271):59-63. Review.
  • Barja G. Endogenous oxidative stress: relationship to aging, longevity and caloric restriction. Ageing Res. 2002; 1:397–411. Review.
  • Merry BJ. Oxidative stress and mitochondrial function with aging the effects of calorie restriction. Aging Cell 2004; 3:7–12.
  • Koizumi A, Weindruch R, Walford RL. Influences of dietary restriction and age on liver enzyme activities and lipid peroxidation in mice. J. Nutr. 1987; 117 (2):361–367.
  • Rao G, Xia E, Nadakavukaren MJ, Richardson A. Effect of dietary on the age-dependent changes in the expression of antioxidant enzymes in rat liver. J. Nutr. 1990;120 (6): 602–609.
  • Mote PL, Grizzle JM, Walford RL, Spindler SR. Influence of age and caloric restriction on expression of hepatic genes for xenobiotic and oxygen metabolizing enzymes in the mouse. J. Gerontol. 1991; 46 (3):95–100.
  • Rojas C, Cadenas S, Perez-Campo R, Lopez-Torres M, Pamplona R, Prat J, Barja G, Relationship between lipid peroxidation, fatty acid composition, and ascorbic acid in the liver during carbohydrate and caloric restriction in mice. Arch. Biochem. Biophys. 1993; 306 (1):59–64.
  • Sohal RS, Ku HH, Agarwal S, Forster MJ, Lal H. Oxidative damage, mitochondrial oxidant generation and antioxidant defenses during aging and in response to food restriction in the mouse. Mech. Ageing Dev. 1994; 74:121–133.
  • Taylor A, Jahngen-Hodge J, Smith D, Palmer VJ, Dallal GE, Lipman RD, Padhye N, Frei B. Dietary restriction delays cataract and reduces ascorbate levels in Emory mice. Exp. Eye Res. 1995b; 61:55–62.
  • Gong X, Shang F, Obin M, Palmer H, Scrofano MM, Jahngen- Hodge J, Smith DE, Taylor A. Antioxidant enzyme activities in lens, liver and kidney of calorie restricted Emory mice. Mech Ageing Dev. 1997; 99(3):181-92.

Effect Of Calorie Restriction On Lipid Peroxidation and Antioxidant Enzymes in Rats

Year 2013, Volume: 3 Issue: 1, 19 - 24, 01.03.2013
https://doi.org/10.5505/sakaryamj.2013.98700

Abstract

Objective: Caloric restriction (CR) is a dietary regimen that restricts calorie intake while maintaining the essential nutritional requirements without disturbing the homeostasis of body. As well as prevention of obesity, CR has been shown to prevent age-related pathologies and delay the aging process. In the present study 60% of caloric intake was restricted for 10 weeks and effect of CR on oxidant and antioxidant markers were determined from their erythrocytes.Materials and Methods: Wistar Albino, 40 male (4-5 months) rats were included and divided into 4 groups as: non-obese (NK), non-obese caloric restricted (ND), obese (OK), obese calorie restricted (OD) groups. At the beginning of the experiment the weight of NK and OK groups were 250±20 gr and 400±20 gr respectively. At the end of 10th week the rats were anaesthetized, euthanized by taking blood from aorta. Hemolyzed erythrocytes were prepared and malondialdehide (MDA), superoxide dismutase (SOD), Catalase (CAT) and finally reduced glutatione (GSH) were determined.Results: While MDA level of OD group was significantly decreased, SOD, CAT and GSH levels of OD group was significantly increased compared to OK group. For ND group, MDA level was significantly decreased and SOD and CAT levels were significantly increased compared to NK group (P

References

  • Gültekin H, Şahin S, Budak N. Beslenme Davranışı: Farmakolojik Hedef Moleküller. Erciyes Üniversitesi Sağlık Bilimleri Dergisi (E.Ü.Journal of Health Sciences) 2004;13(1):77-87. Derleme.
  • Pannacciulli N, Del Parigi A, Chen K, Le DS, Reiman EM, Tataranni PA. Brain abnormalities in human obesity: a voxel-based morphometric study. Neuroimage. 2006; 31(4):1419-25.
  • Craft S. Insulin resistance syndrome and Alzheimer's disease: age- and obesity-related effects on memory, amyloid, and inflammation. Neurobiol Aging. 2005; 26 Suppl 1:65-9.
  • Sriram K, Benkovic SA, Miller DB, O'Callaghan JP. Obesity exacerbates Neuroscience. 2002; 115(4):1335-46. induced neurodegeneration.
  • Çağlayan F, Çağlayan O, Günel E, Çakmak M. The effect of fasting on the oxidant stres, J Med Sci 2001; 21:374-376.
  • Mannarino SC, Amorim MA, Pereira MD, Moradas-Ferreira P, Panek AD, Costa V, Eleutherio EC. Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae. Mech Ageing Dev. 2008; 129(12):700- 5.
  • Gong X, Shang F, Obin M, Palmer H, Scrofano MM, Jahngen- Hodge J, Smith DE, Taylor A. Antioxidant enzyme activities in lens, liver and kidney of calorie restricted Emory mice. Mech Ageing Dev. 1997; 99(3):181-92.
  • Masoro EJ, Iwaski K, Gleiser CA, McMahan CA, Seo E, Yu BP. Dietary modulation of the progression of nephropathy in aging rats: an evaluation of the importance of protein, Am. J. Clin. Nutr. 1989; 49:1217–1227 Scopus.
  • Van Kampen EJ, Zijlstra WG. Determination of hemoglobin and its derivatives. Adv Clin Chem. 1965; 8:141-87.
  • Drapper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186:421–431
  • Woolliams JA, Wiener G, Anderson PH, McMurray CH. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood various breed crosses of sheep. Res Vet Sci. 1983; 34:69–77.
  • Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121–26.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61:882- 90.
  • Das M, Gabriely I, Barzilai N. Caloric restriction, body fat and ageing in experimental models. Obes Rev. 2004; 5(1):13-9.
  • Percy CJ, Brown L, Power DA, Johnson DW, Gobe GC. Obesity and hypertension have differing oxidant handling molecular pathways in age-related chronic kidney disease. Mech Ageing Dev. 2009; 130(3):129-38.
  • Salbe AD, Weyer C, Lindsay RS, Ravssin E, tataranni PA. Assesing risk factors for obesity between childhood adiposity, parental obesity, insülin, Leptin. Pediatrics 2002; 110:299-306.
  • Gülden Burçak, Gülnur Andican. Oksidatif DNA Hasarı ve yaşlanma. (Oxidative DNA damage and aging) Cerrahpaşa J Med 2004; 35:159-169.
  • Silver AE, Bekse SD, Christou DD, Donato AJ, Moreau KL, Eskurza I, Gates PE and Seals DR. 2007. Overweight and Obese Humans Demonstrate Increased Vascular Endothelial NAD(P) H Oxidase-p47 phox Expression and Evidence of Endothelial Oxidative Stres. Circulation. 2007; 115:627-637.
  • Masoro EJ. Caloric restriction-induced life extension of rats and mice: a critique of proposed mechanisms. Biochim Biophys Acta. 2009; 1790(10):1040-8.
  • Ugochukwu NH, Mukes JD, Figgers CL. Ameliorative effects of dietary caloric restriction on oxidative stress and inflammation in the brain of streptozotocin-induced diabetic rats. Clin Chim Acta. 2006; 370(1-2):165-73.
  • Yang MH, Schaich KM. Factors affecting DNA damage caused by lipid hydroperoxides and aldehydes. Free Radic Biol Med. 1996; 20(2):225-36.
  • Sohal RS, Weindruch R. Oxidative stress, caloric restriction, and aging. Science 1996; 5;273(5271):59-63. Review.
  • Barja G. Endogenous oxidative stress: relationship to aging, longevity and caloric restriction. Ageing Res. 2002; 1:397–411. Review.
  • Merry BJ. Oxidative stress and mitochondrial function with aging the effects of calorie restriction. Aging Cell 2004; 3:7–12.
  • Koizumi A, Weindruch R, Walford RL. Influences of dietary restriction and age on liver enzyme activities and lipid peroxidation in mice. J. Nutr. 1987; 117 (2):361–367.
  • Rao G, Xia E, Nadakavukaren MJ, Richardson A. Effect of dietary on the age-dependent changes in the expression of antioxidant enzymes in rat liver. J. Nutr. 1990;120 (6): 602–609.
  • Mote PL, Grizzle JM, Walford RL, Spindler SR. Influence of age and caloric restriction on expression of hepatic genes for xenobiotic and oxygen metabolizing enzymes in the mouse. J. Gerontol. 1991; 46 (3):95–100.
  • Rojas C, Cadenas S, Perez-Campo R, Lopez-Torres M, Pamplona R, Prat J, Barja G, Relationship between lipid peroxidation, fatty acid composition, and ascorbic acid in the liver during carbohydrate and caloric restriction in mice. Arch. Biochem. Biophys. 1993; 306 (1):59–64.
  • Sohal RS, Ku HH, Agarwal S, Forster MJ, Lal H. Oxidative damage, mitochondrial oxidant generation and antioxidant defenses during aging and in response to food restriction in the mouse. Mech. Ageing Dev. 1994; 74:121–133.
  • Taylor A, Jahngen-Hodge J, Smith D, Palmer VJ, Dallal GE, Lipman RD, Padhye N, Frei B. Dietary restriction delays cataract and reduces ascorbate levels in Emory mice. Exp. Eye Res. 1995b; 61:55–62.
  • Gong X, Shang F, Obin M, Palmer H, Scrofano MM, Jahngen- Hodge J, Smith DE, Taylor A. Antioxidant enzyme activities in lens, liver and kidney of calorie restricted Emory mice. Mech Ageing Dev. 1997; 99(3):181-92.
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Details

Primary Language Turkish
Journal Section Articles
Authors

Duygu Kumbul Doğuç This is me

Nigar Yılmaz This is me

Hüseyin Vural This is me

Yusuf Kara This is me

Publication Date March 1, 2013
Submission Date September 7, 2015
Published in Issue Year 2013 Volume: 3 Issue: 1

Cite

AMA Doğuç DK, Yılmaz N, Vural H, Kara Y. Sıçanlarda Kalori Kısıtlamasının Lipid Peroksidasyonu Ve Antioksidan Enzimlere Etkisi. Sakarya Tıp Dergisi. March 2013;3(1):19-24. doi:10.5505/sakaryamj.2013.98700

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