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Taksifolinin Sıçanlarda Etanolle İndüklenen Oksidatif Beyin Hasarına Etkisi

Year 2018, Volume: 8 Issue: 3, 638 - 643, 30.09.2018
https://doi.org/10.31832/smj.457718

Abstract

Amaç: Araştırmalar,
alkolun en şiddetli toksik etkisinin merkezi sinir sistemi (MSS) üzerinde
olduğunu göstermiştir.
Alkolun MSS üzerindeki toksik etkisinin
patogenezinde serbest oksijen radikal (SOR) üretiminin artışı, endojen
antioksidan üretiminin baskılanması sorumlu tutulmaktadır. Amacımız Taksifolinin sıçanlarda etanolle indüklenen
oksidatif beyin hasarına etkisini biyokimyasal olarak araştırmaktır. Gereç ve Yöntemler:
Deney hayvanları sağlıklı (SG), tek başına
etanol alan kontrol (EtOH) ve taksifolin +etanol verilen (TEtOH) gruplara
ayrıldı. Etanol ve Taksifolinin belirlenen sürede verilmesi sonunda hayvanlar
yüksek doz anestezi ile öldürülerek beyin dokuları çıkarıldı. Çıkarılan beyin
dokularında lipid peroksidasyon ürünü olan malondialdehid (MDA) ve endojen
antioksidan olan total glutatyon (tGSH) ölçümleri yapıldı.
Bulgular: Etanol
verilen hayvan grubunda MDA miktarı sağlıklı ve taksifolin
grubuna göre anlamlı yüksek (p<0.001),
tGSH ise anlamlı düşük (p<0.001)
bulundu. Sonuç: Sonuçlarımız, taksifolinin
beyin dokusunu etanolun oksidatif hasarına karşı koruduğunu işaret etmektedir.000

References

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  • Referans3. Palmisano M, Pandey SC. Epigenetic mechanisms of alcoholism and stress-related disorders. Alcohol, 2017, 60: 7-18.
  • Referans4. Soysal Z, Çakalır C, Gürsel Ç. Adli Tıp. 1. baskı. İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Yayınları; 1999. s: 29-44.
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  • Referans6. Wang X, Michaelis EK. Selective neuronal vulnerability to oxidative stress in the brain. Front Aging Neurosci 2010; 2: 12.
  • Referans7. Kim GH, Kim JE, Rhie SJ, Yoon S. The role of oxidative stress in neurodegenerative diseases. Exp Neurobiol 2015; 24: 325-340.
  • Referans8. Haorah J, Ramirez SH, Floreani N, Gorantla S, Morsey B, Persidsky Y. Mechanism of alcohol-induced oxidative stress and neuronal injury. Free Radic Biol Med 2008; 45: 1542-1550.
  • Referans9. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20: 933-956.
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  • Referans11. Bimpilas A, Tsimogiannis D, Balta-Brouma K, Lymperopoulou T, Oreopoulou V. Evolution of phenolic compounds and metal content of wine during alcoholic fermentation and storage. Food Chem 2015; 178: 164-171.
  • Referans12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358.
  • Referans13. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.Anal Biochem 1968; 25: 192-205.
  • Referans14. Kunak CS, Kukula O, Mutlu E, Genç F, Güleç Peker G, Kuyrukluyıldız U, et al. The effect of etoricoxib on hepatic ischemia-reperfusion injury in rats. Oxid Med Cell Longev 2015, 2015.
  • Referans15. Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med 2013; 45: 47.
  • Referans16. Nordmann R. Oxidative stress from alcohol in the brain. Alcohol and Alcoholism 1987;1: 75-82.
  • Referans17. Wu Y, Li S, Liu J, Liu X, Ruan W, Lu J-W, Liu Y, Lawson T, Shimoni O, Lovejoy D. Stilbenes from Veratrum maackii Regel. Protect against Ethanol-Induced DNA Damage in Mouse Cerebellum and Cerebral Cortex. ACS Chem Neurosci 2018.
  • Referans18. Crews FT, Nixon K. Mechanisms of neurodegeneration and regeneration in alcoholism. Alcohol & Alcoholism 2008; 44: 115-127.
  • Referans19. Büyükuslu N, Yiğitbaşı T. Reaktif oksijen türleri ve obezitede oksidatif stres. Marmara Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 2015;5:197-203.
  • Referans20. Repetto M, Semprine J, Boveris A. Lipid peroxidation: chemical mechanism, biological implications and analytical determination. İçinde:Lipid peroxidation, InTech, 2012.
  • Referans21. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014; 1-31.
  • Referans22. Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCORD JM, Harman D. Oxygen radicals and human disease. Ann Intern Med 1987; 107: 526-545.
  • Referans23. Di Mascio P, Murphy ME, Sies H. Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols.Am JClin Nutr 1991; 53: 194S-200S.
  • Referans24. Larson RA. The antioxidants of higher plants. Phytochemistry 1988; 27: 969-978.
  • Referans25. Hamid A, Ibrahim FW, Ming TH, Nasrom MN, Eusoff N, Husain K, Latif MA. Zingiber zerumbet L.(Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity. BMC Complement Altern Med 2018; 18: 101.
  • Referans26. Pereira RB, Sousa C, Costa A, Andrade PB, Valentão P. Glutathione and the antioxidant potential of binary mixtures with flavonoids: synergisms and antagonisms. Molecules 2013; 18: 8858-8872.
Year 2018, Volume: 8 Issue: 3, 638 - 643, 30.09.2018
https://doi.org/10.31832/smj.457718

Abstract

References

  • Referans1. Stein E. Textbook of clinical chemistry. WB Saunders (eds),.Philadelphia, NW, USA,1986. pp: 879-886.
  • Referans2. Vural N. Alifatik Alkoller. Ankara Üniversitesi Eczacılık Fakültesi Yayınları; Toksikoloji. Ankara; 2005. s: 293-305.
  • Referans3. Palmisano M, Pandey SC. Epigenetic mechanisms of alcoholism and stress-related disorders. Alcohol, 2017, 60: 7-18.
  • Referans4. Soysal Z, Çakalır C, Gürsel Ç. Adli Tıp. 1. baskı. İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Yayınları; 1999. s: 29-44.
  • Referans5. Gonthier B, Signorini‐Allibe N, Soubeyran A, Eysseric H, Lamarche F, Barret L. Ethanol Can Modify the Effects of Certain Free Radical‐Generating Systems on Astrocytes. Alcohol Clin Exp Res,2004; 28: 526-534.
  • Referans6. Wang X, Michaelis EK. Selective neuronal vulnerability to oxidative stress in the brain. Front Aging Neurosci 2010; 2: 12.
  • Referans7. Kim GH, Kim JE, Rhie SJ, Yoon S. The role of oxidative stress in neurodegenerative diseases. Exp Neurobiol 2015; 24: 325-340.
  • Referans8. Haorah J, Ramirez SH, Floreani N, Gorantla S, Morsey B, Persidsky Y. Mechanism of alcohol-induced oxidative stress and neuronal injury. Free Radic Biol Med 2008; 45: 1542-1550.
  • Referans9. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20: 933-956.
  • Referans10. Cotelle N. Role of flavonoids in oxidative stress. Curr Top Med Chem 2001; 1: 569-590.
  • Referans11. Bimpilas A, Tsimogiannis D, Balta-Brouma K, Lymperopoulou T, Oreopoulou V. Evolution of phenolic compounds and metal content of wine during alcoholic fermentation and storage. Food Chem 2015; 178: 164-171.
  • Referans12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358.
  • Referans13. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.Anal Biochem 1968; 25: 192-205.
  • Referans14. Kunak CS, Kukula O, Mutlu E, Genç F, Güleç Peker G, Kuyrukluyıldız U, et al. The effect of etoricoxib on hepatic ischemia-reperfusion injury in rats. Oxid Med Cell Longev 2015, 2015.
  • Referans15. Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med 2013; 45: 47.
  • Referans16. Nordmann R. Oxidative stress from alcohol in the brain. Alcohol and Alcoholism 1987;1: 75-82.
  • Referans17. Wu Y, Li S, Liu J, Liu X, Ruan W, Lu J-W, Liu Y, Lawson T, Shimoni O, Lovejoy D. Stilbenes from Veratrum maackii Regel. Protect against Ethanol-Induced DNA Damage in Mouse Cerebellum and Cerebral Cortex. ACS Chem Neurosci 2018.
  • Referans18. Crews FT, Nixon K. Mechanisms of neurodegeneration and regeneration in alcoholism. Alcohol & Alcoholism 2008; 44: 115-127.
  • Referans19. Büyükuslu N, Yiğitbaşı T. Reaktif oksijen türleri ve obezitede oksidatif stres. Marmara Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 2015;5:197-203.
  • Referans20. Repetto M, Semprine J, Boveris A. Lipid peroxidation: chemical mechanism, biological implications and analytical determination. İçinde:Lipid peroxidation, InTech, 2012.
  • Referans21. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014; 1-31.
  • Referans22. Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCORD JM, Harman D. Oxygen radicals and human disease. Ann Intern Med 1987; 107: 526-545.
  • Referans23. Di Mascio P, Murphy ME, Sies H. Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols.Am JClin Nutr 1991; 53: 194S-200S.
  • Referans24. Larson RA. The antioxidants of higher plants. Phytochemistry 1988; 27: 969-978.
  • Referans25. Hamid A, Ibrahim FW, Ming TH, Nasrom MN, Eusoff N, Husain K, Latif MA. Zingiber zerumbet L.(Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity. BMC Complement Altern Med 2018; 18: 101.
  • Referans26. Pereira RB, Sousa C, Costa A, Andrade PB, Valentão P. Glutathione and the antioxidant potential of binary mixtures with flavonoids: synergisms and antagonisms. Molecules 2013; 18: 8858-8872.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Articles
Authors

Aslı Özbek Bilgin 0000-0001-9027-4299

Renad Mammadov 0000-0002-5785-1960

Bahadır Suleyman This is me 0000-0001-5795-3177

Publication Date September 30, 2018
Submission Date September 6, 2018
Published in Issue Year 2018 Volume: 8 Issue: 3

Cite

AMA Özbek Bilgin A, Mammadov R, Suleyman B. Taksifolinin Sıçanlarda Etanolle İndüklenen Oksidatif Beyin Hasarına Etkisi. Sakarya Tıp Dergisi. September 2018;8(3):638-643. doi:10.31832/smj.457718

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