Oosit İn Vitro Maturasyonunda Fosfatidilinositol 4,5- Bifosfat (PIP2) Kullanımının Etkisi

Year 2020, Volume: 10 Issue: 3, 474 - 483, 15.09.2020

Filiz Tepekoy

https://doi.org/10.31832/smj.728050

Abstract

Amaç: Hücresel protein dengesi üzerinde etkili olduğu bilinen fosfatidilinositol 4,5- bifosfat (PIP2)’nin, 8-13 yaş aralığındaki sığır oositlerinin in vitro maturasyon (IVM) sistemine farklı konsantrasyonlarda eklenmesinin, farklı IVM sürelerinde oosit canlılığı ve olgunlaşması üzerindeki etkisinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntemler: Mezbahada kesimi gerçekleşen sığırlardan elde edilen ovaryumlardan aspire edilen kumulus oosit komplekslerinden kumulus hücrelerinin mekanik olarak uzaklaştırılmasıyla soyulan oositler IVM medyumu içerisine alınarak 6 saat ve 24 saat süreyle kültüre edilmiştir. IVM medyumu içerisine 0,1µM (P1), 0,5 µM (P2), 1 µM (P3) ve 5 µM (P4) konsantrasyonlarda PIP2 eklenmiştir. Kültür sürecinin sonunda tüm gruplardaki 1. mayoz bölünmeyi tamamlayabilen olgunlaşmış oositlerin, olgunlaşmamış ancak canlılığını korumuş oositlerin ve ölü oositlerin oranları belirlenmiştir.
Bulgular: 6 saatlik IVM sonrasında en yüksek olgunlaşma oranının P1 grubunda olduğu, ölü oosit oranının ise P4 grubunda en yüksek olduğu belirlenmiştir. 24 saatlik IVM sonrasında da en yüksek olgunlaşma oranı P1 grubunda görülmüş, P4 grubunda ölü oosit oranının en fazla, P1 grubunda ise en düşük düzeyde olduğu saptanmıştır.
Sonuç: PIP2’nin düşük konsantrasyonlarda IVM medyumuna eklenmesi oosit olgunlaşma ve sağ kalım oranlarını artırmaktadır.

Keywords

oosit, maturasyon, IVM

Supporting Institution

Tübitak

Project Number

118S772

Thanks

Çalışmanın gerçekleşmesi sırasındaki teknik desteklerinden dolayı Narmanlar ET kombinası yetkilisi Yusuf Narman ve veteriner hekim Yılmaz Küçükafacan‘a teşekkür ediyorum.

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