ASSOCIATION BETWEEN MCT1 GENE POLYMORPHISM (rs1049434) WITH THE ATHLETIC PERFORMANCE OF ELITE TRACK AND FIELD ATHLETES

Year 2023, , 127 - 134, 31.03.2023

Celal Bulğay , Erdal Zorba , Işık Bayraktar , Hasan Huseyin Kazan , Korkut Ulucan , Mehmet Ali Ergun

https://doi.org/10.33689/spormetre.1198404

Cited By: 1

Abstract

Monocarboxylate transporter 1 (MCT1; SLC16A1) is a proton-dependent cotransporter/exchanger, located on the apical membrane of cells. MCT1 is able to transport several monocarboxylates including lactate, pyruvate and acetate, which makes this protein critical in terms of the athletic performances. The rs1049434 polymorphism in the MCT1gene was frequently associated with the performance of the athletes in different populations. The present study aims to decipher any possible association of the rs1049434 polymorphism with the personal best of elite track and field athletes. A total of sixty elite athletes (31 sprint/power and 29 endurance) and twenty control/sedentary with the ages of 18-35 voluntarily participated in the study. The International Association of Athletics Federations (IAAF) score scale was used to determine the performance levels of the personal best (PB) of the athletes. Whole exome sequencing (WES) was performed by the genomic DNA isolated from blood of the participants. Sport type, sex and PB were chosen as the parameters to compare within and between the groups by logistic regression models. The sex was not the criterion that was significantly different between or within the groups. Although PB scores were not significant within the sprint/power group, it was significant within the endurance group by the codominant (p=0.044), dominant (p=0.016) and over-dominant (p=0.048) models. The rs1049434 polymorphism in the MCT1 gene may be linked to the PB of the endurance athletes. However, other genetic alterations should be regarded to conclude the effect of this polymorphism. The multi-factorial genetic background that could associate with the athletic performance is still under investigation in our research group. Nevertheless, further studies with more participants are needed.

Keywords

Athletics, athlete, runner, rs1049434, polymorphism, MCT1, SLC16A1

Supporting Institution

Gazi University Rectorate

Project Number

TCD-2021-7116

ELİT ATLETLERDE MCT1 GEN POLİMORFİZMİNİN (rs1049434) ATLETİK PERFORMANS İLE İLİŞKİSİNİN ARAŞTIRILMASI

Abstract

Monocarboxylate transporter 1 (MCT1; SLC16A1) is a proton-dependent cotransporter/exchanger, located on the apical membrane of cells. MCT1 is able to transport several monocarboxylates including lactate, pyruvate and acetate, which makes this protein critical in terms of the athletic performances. The rs1049434 polymorphism in the MCT1gene was frequently associated with the performance of the athletes in different populations. The present study aims to decipher any possible association of the rs1049434 polymorphism with the personal best of elite track and field athletes. A total of sixty elite athletes (31 sprint/power and 29 endurance) and twenty control/sedentary with the ages of 18-35 voluntarily participated in the study. The International Association of Athletics Federations (IAAF) score scale was used to determine the performance levels of the personal best (PB) of the athletes. Whole exome sequencing (WES) was performed by the genomic DNA isolated from blood of the participants. Sport type, sex and PB were chosen as the parameters to compare within and between the groups by logistic regression models. The sex was not the criterion that was significantly different between or within the groups. Although PB scores were not significant within the sprint/power group, it was significant within the endurance group by the codominant (p=0.044), dominant (p=0.016) and over-dominant (p=0.048) models. The rs1049434 polymorphism in the MCT1 gene may be linked to the PB of the endurance athletes. However, other genetic alterations should be regarded to conclude the effect of this polymorphism. The multi-factorial genetic background that could associate with the athletic performance is still under investigation in our research group. Nevertheless, further studies with more participants are needed.

Keywords

Atletizm, sporcu, koşucu, rs1049434, polimorfizm, MCT1, SLC16A1

Project Number

TCD-2021-7116

References

• Ahmetov, I. I., Fedotovskaya, O. N. (2015). Current Progress in Sports Genomics. In Advances in Clinical Chemistry (Vol. 70, pp. 247–314). Academic Press Inc.
• Akkoc, O., Birlik, A., Doğan, C. S., Kırandı, O., Ulucan, K. (2020). Determination of IL-6, HIF1A, MCT1, PPAR-a Polymorphism Distribution in Turkish Ironman Triathlon Athletes. Journal of Sports Education 4(1), 1-7.
• Ben-Zaken, S., Eliakim, A., Nemet, D., Rabinovich, M., Kassem, E., and Meckel, Y. (2015). ACTN3 polymorphism: comparison between elite swimmers and runners. Sports Medicine-Open 1(1), 1–8.
• Bishop, D., Edge, J., Thomas, C., Mercier, J. (2007). High-intensity exercise acutely decreases the membrane content ofMCT1 and MCT4 and buffer capacity in human skeletal muscle. J Appl Physiol 102, 616–621.
• Bulğay, B., Zorba, E., Ergün, M. A. (2021). Effect of MCT1 Gene on Athlete Performance: A Review Study. Gazi madical journal 614–617.
• Bulğay, C., Çetin, E., Orhan, Ö. & Ergün, M. A. (2020). The effects of the ACTN3 and ACE genes on the sportive performance of athletes. İnönü Üniversitesi Beden Eğitimi ve Spor Bilimleri Dergisi, 7(1), 1–12.
• Bulğay, C., Zorba, E., Akman, O., Bayraktar, I., Kazan, H. H., Ergun, M. A. & Ulucan, K. (2022). Evaluation of Association between PPARGC1A Gene Polymorphism and Competitive Performance of Elite Athletes. Gazi Beden Eğitimi ve Spor Bilimleri Dergisi, 27 (4), 323-332.
• Bulgay, C., & Ergun, MA. (2022). Atletik Performans, Genetik ve Epigenetik Üçlüsü. Egzersiz Fizyolojisi ve Temel Kavramlar. Ankara: Efe Akademi Yayınları.
• Cupeiro, R., Benito, P. J., Maffulli, N., Calderón, F. J., González-Lamuño, D. (2010). MCT1 genetic polymorphism influence in high intensity circuit training: a pilot study. J Sci Med Sport 13:526–530.
• Cupeiro, R., Gonzalez-Lamuno, D., Amigo, T., Peinado, A., Ruiz, J., Ortega, F., Benito, P. (2012). Influence of the MCT1-T1470A polymorphism (rs1049434) on blood lactate accumulation during different circuit weight trainings in men and women. Journal of Science and Medicine in Sport 15(6), 541–547.
• Fedotovskaya, O., Mustafina, L., Popov, V., Vinogradova, O., Ahmetov, I. (2014). A Common Polymorphism of the MCT1 Gene and Athletic Performance. International Journal of Sports Physıology and Performance 9, 173–180.
• Girard, O., Mendez-Villanueva, A., Bishop, D. (2011). Repeated-sprint ability—part I: factors contributing to fatigue. Sports Med 41, 673–694.
• Goran, S., Igor, J., Sergen, M., Dragan, M. (2009). Fitness Profiling in Soccer: Physical and Physiologic Characteristics of Elite Players. Journal of Strength and Conditioning Research 23(7), 1947–1953.
• Guilherme, P. L. F., Bosnyák, E., Semenova, E. A., Szmodis, M., Kostryukova, S., Borisov, O. V. Larin, A. K., Andryushchenko, L. B., Akimov, E. B., Paulo, S. (2021). The MCT1 gene Glu490Asp polymorphism (rs1049434) is associated with endurance athlete status , lower blood lactate accumulation and higher maximum oxygen uptake. 465–474.
• Kikuchi, N., Fuku, N., Matsumoto, R., Matsumoto, S., Murakami, H., Miyachi, M., Nakazato, K. (2017). The Association Between MCT1 T1470A Polymorphism and Power-Oriented Athletic Performance. Genetics & Molecular Biology 38(1), 76–80.
• Kline, R. (2011). Methodology in the Social Sciences. Principles and practice of structural equation modeling (3.). Guilford Press.
• Knutttgen, H. (2007). Strength training andaerobic exercise: comparison andcontrast. J Strength Cond Res 21(3), 973–978.
• Massidda, M., Flore, L., Kikucji, N., Scorcu, M., Piras, F., Cugia, P., Cieszczyk, P., Tocco, F., Calo, C. (2021) Influence of the MCT1‐T1470A polymorphism (rs1049434) on repeated sprint ability and blood lactate accumulation in elite football players: a pilot study. European Journal of Applied Physiology 121, 3399–3408.
• Merezhinskaya, N., Fishbein, W., Davis, J., Foellmer, J. (2000). Mutations in MCT1 cDNA in patients with symptomatic deficiency in lactate trans- port. Muscle Nerve, 23, 90–97.
• Pilagaard, H., Bangsbo, J., Richter, E., Juel, C. (1994). Lactate trans- port studied in sarcolemmal giant vesicles from human muscle biopsies: relation to training status. J Appl Physiol 77(4), 1858–1862.
• Piscina-Viudez, X., Alvarez-Herms, J., Bonilla, D., Castaneda-Babarro, A., Larruskain, J., Diaz-Ramirez, J. (2021). Putative Role of MCT1 rs1049434 Polymorphism in High-Intensity Endurance Performance: Concept and Basis to Understand Possible Individualization Stimulus. Sports 9, 143.
• Saito, M., Ginszt, M., Massidda, M., et al. (2021). Association between MCT1 T1470A polymorphism and climbing status in Polish and Japanese climbers. Biol Sport 38(2), 229–234.
• Sawczuk, M., Banting, L., Cieszczyk, P., Maciejewska-Karlowska, A., Zarebska, A., Leonska-Duniec, A., Jastrzebski, Z., Bishop, J., Eynon, N. (2015). Journal of Science and Medicine in Sport MCT1 A1470T : A novel polymorphism for sprint performance ? 18, 114–118.
• Solé, X., Guinó, E., Valls, J., Iniesta, R., Moreno, V. (2006). SNPStats: a web tool for the analysis of association studies. Bioinformatics, 22(15), 1928-1929.
• Spiriev, B. (2014). IAAF Scoring Tables of Athletics. 368.
• Van der Auwera, G., Carneiro, M., Harl, C., Poplin, R., Del Angel, G., Levy-Moonshine, A. . . . , DePristo, M.. (2013) From FastQ Data to High-Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline. Curr. Protoc. Bioinform, Wiley Online Library.
• Wang, K., Li, M., Kakonarson, H. (2010). ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Research 38(16), e168.
• Yıldırım, D. S., Erdoğan, M., Dalip, M., Bulğay, C., & Cirit, M. (2022). Evaluation of the soldier's physical fitness test results (strength endurance) ın relation to genotype: longitudinal study. Egypt J Med Hum Genet, 23(114), 2-9.
• Zelka, M. K., Kaşıkçı, E. S., Doğan, C. S., Kapıcı, S., Ulucan, K., Konuk, M. (2019) Heterozygous Genotype oF Monocarboxyl Transferase 1 (Rs1049434) Polymorphısm Commons in A Turkısh Athlete Cohort. The Journal of Neurobehavioral Sciences 6(2), 129-132.