Unlocking The Therapeutic Potential of Silene compacta: A Comparative Study of Antioxidant and Enzyme Inhibitory Activities Across Solvent Extracts

Abstract

There has been a growing focus on the pharmacological research of medicinal plants, particularly their physiological and pharmacological effects. This study explores the chemical composition, antioxidant, and enzyme inhibitory activities of water, methanol, and ethyl acetate extracts from Silene compacta. The methanol extract demonstrated the highest total phenolic (30.88 mg GAEs/g) and flavonoid (50.19 mg REs/g) contents, translating to superior antioxidant activities in the DPPH• (45.82 mg TEs/g) and ABTS•+ (57.03 mg TEs/g) assays, as well as in CUPRAC and FRAP assays. In contrast, the ethyl acetate extract, while lower in phenolics, exhibited remarkable activity in the phosphomolybdenum assay (275.10 mg TEs/g) and showed strong enzyme inhibitory activities, particularly against α-glucosidase (1470.25 mg ACEs/g) and AChE (3.11 mg GALAEs/g). The water extract, with intermediate phenolic content, displayed balanced antioxidant properties across different assays, but its enzyme inhibitory effects were weaker. Correlation analysis revealed strong positive relationships between total phenolic content and most antioxidant assays, underscoring the importance of phenolic compounds in contributing to the observed bioactivities. The findings suggest that methanol extracts are particularly promising for applications requiring potent antioxidant properties, while ethyl acetate extracts might be more suited for enzyme inhibition-related applications. Future research should consider in vivo studies and explore the synergistic effects among different phytochemicals to fully understand the therapeutic potential of S. compacta.

Keywords

• Silene compacta
• antioxidant activity
• enzyme inhibition
• phenolic content
• phytochemical analysis

Silene compacta'nın Terapötik Potansiyelinin Ortaya Çıkarılması: Çözücü Özütleri Arasında Antioksidan ve Enzim İnhibitör Aktivitelerin Karşılaştırmalı Bir Çalışması

Abstract

Son yıllarda, tıbbi bitkilerin farmakolojik araştırmalarına, özellikle fizyolojik ve farmakolojik etkilerine artan bir ilgi gösterilmiştir. Bu çalışma, Silene compacta bitkisinin su, metanol ve etil asetat özütlerinin kimyasal bileşimini, antioksidan ve enzim inhibitör aktivitelerini incelemektedir. Metanol özütü, toplam fenolik (30.88 mg GAEs/g) ve flavonoit (50.19 mg REs/g) içerikleri açısından en yüksek değerlere sahip olup, DPPH• (45.82 mg TEs/g) ve ABTS•+ (57.03 mg TEs/g) testlerinde, ayrıca CUPRAC ve FRAP testlerinde de üstün antioksidan aktiviteler göstermiştir. Buna karşın, etil asetat özütü, fenolik içerik açısından daha düşük olmasına rağmen, fosfomolibdenum testinde (275.10 mg TEs/g) dikkat çekici bir aktivite sergilemiş ve özellikle α-glukozidaz (1470.25 mg ACEs/g) ve AChE (3.11 mg GALAEs/g) üzerinde güçlü enzim inhibitör aktiviteleri göstermiştir. Su özütü, orta düzeyde fenolik içerik ile farklı testlerde dengeli antioksidan özellikler sergilemiş, ancak enzim inhibitör etkileri daha zayıf olmuştur. Korelasyon analizi, toplam fenolik içerik ile çoğu antioksidan test arasında güçlü pozitif ilişkiler olduğunu ortaya koymuş, bu da fenolik bileşiklerin gözlemlenen biyolojik aktiviteler üzerindeki önemini vurgulamaktadır. Bulgular, metanol özütünün güçlü antioksidan özellikler gerektiren uygulamalar için özellikle umut verici olduğunu, etil asetat özütünün ise enzim inhibisyonu ile ilgili uygulamalar için daha uygun olabileceğini önermektedir. Gelecek araştırmalar, S. compacta'nın terapötik potansiyelini tam olarak anlamak için in vivo çalışmaları ve farklı fitokimyasallar arasındaki sinerjik etkileri keşfetmeyi dikkate almalıdır.

Keywords

• Silene compacta
• antioksidan aktivite
• enzim inhibisyonu
• fenolik içerik

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