Laktik Asit Tayini için Magnetit Nanopartiküller ve Prusya Mavisi ile Modifiye Edilmiş Amperometrik Biyosensör

Abstract

Laktatoksidaz enzimi (LOx), Fe3O4 nanopartiküller ve Prusya mavisi (PB) ile modifiye edilmiş karbon pasta elektrot laktik asit tayini için amperometrik biyosensör olarak araştırıldı. LOx karbon pasta matrikse glutaraldehit (GA) ve sığır serum albümin (BSA) kullanılarak çapraz bağlama immobilize edildi. Yalın ve modifiye edilmiş karbon pasta elektrotların elektron transfer özellikleri elektrokimyasal empedans spektroskopisi (EIS) ve dönüşümlü voltametri (DV) kullanılarak incelendi. Enzim elektrodun analitik performansını etkileyen parametreler detaylı olarak incelendi ve optimize edildi. Laktik aside optimum cevap 0.05 M pH 5.5 fosfat tamponu içinde Ag/AgCl elektroda karşı +0.10 V’da elde edildi. Biyosensör laktik aside 7.5×10-6 −1.3×10-4 M aralığında, 5.9×10-7 M gözlenebilme sınırı ve 0.998 korelasyon katsayısı ile doğrusal cevap gösterdi. Bozucu türlerin etkisi, biyosensörün çalışma ve depolama kararlılıkları da değerlendirildi. Biyosensör +4°C de saklandığında 1 hafta sonra başlangıç aktivitesinin %5.4’ünü kaybetti.

Keywords

• Laktik asit, Amperometri, Enzim elektrot, Fe3O4 nanopartiküller, Prusya mavisi

Magnetite Nanoparticles and Prussian Blue Modified Amperometric Biosensor for Lactic Acid Determination

Abstract

Carbon paste electrode modified with lactate oxidase (LOx) enzyme, Fe3O4 nanoparticles and Prussian blue (PB) was investigated as amperometric biosensor for lactic acid. LOx was immobilized by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA) into carbon paste matrix. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to investigate the electron transfer properties of bare and modified carbon paste electrodes. The parameters affecting the analytical performance of the enzyme electrode have been investigated in detail and optimized. The optimum response to lactic acid was obtained in 0.05 M phosphate buffer at pH 5.5 when polarized at +0.10 V vs. Ag/AgCl. The biosensor provides a linear response for lactic acid in the concentration range of 7.5×10-6-1.3×10-4 M with a detection limit of 5.9×10-7 M and correlation coefficient of 0.998. The effects of interferents, the operational and storage stabilities of the biosensor were also evaluated. The biosensor showed 5.4% loss in its initial activity after one week when stored at +4°C.

Keywords

• Lactic acid, Amperometry, Enzyme electrode, Fe3O4 nanoparticles, Prussian blue

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