ONOPORDUM ANATOLICUM TOHUMLARININ ANTIOKSİDAN AKTİVİTELERİ

Abstract

Onopordum cinsi Türkiye’de ondokuz takson ile temsil edilmekte ve Anadolu’da tıbbi amaçla geleneksel olarak kullanılmaktadır. O. anatolicum tohumlarının etil alkol ve su özütlerinin antioksidan özellikleri β-karoten/linoleik asit, DPPH serbest radikal süpürme, indirgeme gücü, ve metal şelatma testlerini içeren farklı kimyasal testlerle araştırıldı. Ayrıca, özütlerin toplam fenolik ve flavonoid içerkikleri de belirlendi. Genel olarak, etil alkol özütü yüksek toplam fenolik içerikle önemli antioksidan aktivite (metal şelatlama dışında) sergilemiştir. Bununla birlikte, su özütü çalışılan örnekler arasında en güçlü şelatlama aktivitesine sahiptir. Bu çalışma O. anatolicum özütlerinin doğal antioksidanların yeni bir kaynağı olarak gıda ve farmakoloji endüstrisinde kullanılabileceğini önermektedir.

Keywords

• Antioksidan, Çözücü, İndirgeme gücü, Onopordum, Serbest radikal, Türkiye

Antioxidant Activities of Onopordum anatolicum seeds

Abstract

The genus Onopordum contained nineteen taxa in Turkey, which are traditionally used for medicinal purposes in Anatolia. Antioxidant activities of ethyl alcohol and water extracts from O. anatolicum seeds were investigated by different chemical assays including β-carotene/linoleic acid, DPPH radical scavenging, reducing power and metal chelating. Also, total phenolic and flavonoid content were determined for the extracts. Generally, ethyl alcohol extract showed significantly antioxidant activity (except for metal chelating) with high total phenolic content. However, the water extract had the strongest metal activity among these samples. This study suggested that O. anatolicum extracts could be considered as novel a source of natural antioxidants in food and pharmacological industries

Keywords

• Antioxidant, Solvents, Reducing power, Onopordum, Free radical, Turkey

References

1. Altundag, E., & Ozturk, M. (2011). Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia-Social and Behavioral Sciences, 19, 756-777.
2. Aruoma, O.I., 1996. Assessment of potential prooxidant and antioxidant actions, Journal of the American Oil Chemists Society, 73(12), 1617-1625.
3. Arvouet-Grand, A, Vennat, B, Pourrat, A, Legret, P (1994). Standardisation d’un extrait de propolis et identification des principaux constituants. Journal De Pharmacie De Belgique 49, 462–468.
4. Chavan, J. J., Gaikwad, N. B., Kshirsagar, P. R., & Dixit, G. B. (2013). Total phenolics, flavonoids and antioxidant properties of three Ceropegia species from Western Ghats of India. South African Journal of Botany, 88, 273-277.
5. Dapkevicius, A, Venskutonis, R, Van Beek, TA, Linssen, PH (1998). Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture 77, 140-146.
6. Davis PH, Mill RR, Tan K (eds) (1988). Flora of Turkey and the east Aegean Islands (Suppl. 1.), Vol. 10. Edinburgh: Edinburgh University Press.
7. Deliorman‐Orhan, D., Şenol, S., Kartal, M., & Orhan, I. (2009). Assessment of antiradical potential of Calluna vulgaris (L.) Hull and its major flavonoid. Journal of the Science of Food and Agriculture, 89(5), 809- 814.
8. Dinis, TCP, Madeira, VMC, Almeida, LM (1994). Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics 315, 161–169.

9. Fridovich, I., 1995. Superoxide radical and superoxide dismutases. Annual Review of Biochemistry, 64, 97–112.
10. Halliwell, B., 1994. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence. Lancet, 344, 721– 724. 23,76 7,32 4,16 Etil alkol Su Etil alkol Su
11. Toplam fenolik (mg PEs/g özüt)
12. Toplam flavonoit (mg QEs/g özüt)