TR
EN
Abstract
Katalaz, kısmi indirgenmiş oksijen türleri ve H2O2 nin yükseltgen etkisinden dokuların korunmasında önemli rol oynar. Tavuk karaciğer katalazı, (NH4)2SO4 çöktürmesi, etonol-kloroform muamelesi, CM-Selükoz ve Sephadeks G-200 kromotografisi kullanılarak saflaştırıldı. Jel filitrasyon kromotografisi ile tavuk karaciğer katalazının molekül ağırlığı yaklaşık olarak 235000 Da bulundu. SDS-jel elektroforezi sonucu tavuk karaciğer katalazının molekül ağırlığı 57500 Da olarak bulundu ve 4 alt birimden oluştuğu tespit edildi. Spesifik aktivite 55765.5 U/mg olarak saptandı. Hem içeriğinin kabaca ölçülmesini sağlayan A407/A270 oranı 0.52 olarak bulundu. Enzim için Km değeri 100 mM olarak belirlendi. Enzimin 7.0-10.0 aralığında geniş bir pH optimumuna sahip olduğu görüldü. Tavuk karaciğer katalaz enziminin optimum sıcaklığı 25 °C olarak tespit edildi. Saflaştırılmış enzim aktivitesi KCN, NaN3, β-merkaptoetanol, DTT, iyodoasetamit tarafından inhibe edildi, fakat sodyum dietilditiyokarbamatın enzimi inhibe etmediği görüldü.
Keywords
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
December 1, 2003
Submission Date
December 1, 2003
Acceptance Date
-
Published in Issue
Year 2003 Volume: 1 Number: 21
APA
Aydemir, T., Kuru, K., & Çınar, S. (2003). Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity. Selcuk University Journal of Science Faculty, 1(21), 63-74. https://izlik.org/JA85US64AJ
AMA
1.Aydemir T, Kuru K, Çınar S. Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity. Selcuk University Journal of Science Faculty. 2003;1(21):63-74. https://izlik.org/JA85US64AJ
Chicago
Aydemir, Tülin, Kevser Kuru, and Seda Çınar. 2003. “Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity”. Selcuk University Journal of Science Faculty 1 (21): 63-74. https://izlik.org/JA85US64AJ.
EndNote
Aydemir T, Kuru K, Çınar S (December 1, 2003) Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity. Selcuk University Journal of Science Faculty 1 21 63–74.
IEEE
[1]T. Aydemir, K. Kuru, and S. Çınar, “Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity”, Selcuk University Journal of Science Faculty, vol. 1, no. 21, pp. 63–74, Dec. 2003, [Online]. Available: https://izlik.org/JA85US64AJ
ISNAD
Aydemir, Tülin - Kuru, Kevser - Çınar, Seda. “Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity”. Selcuk University Journal of Science Faculty 1/21 (December 1, 2003): 63-74. https://izlik.org/JA85US64AJ.
JAMA
1.Aydemir T, Kuru K, Çınar S. Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity. Selcuk University Journal of Science Faculty. 2003;1:63–74.
MLA
Aydemir, Tülin, et al. “Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity”. Selcuk University Journal of Science Faculty, vol. 1, no. 21, Dec. 2003, pp. 63-74, https://izlik.org/JA85US64AJ.
Vancouver
1.Tülin Aydemir, Kevser Kuru, Seda Çınar. Purification and Partial Characterisation of Catalase from Chicken Liver and the Inhibition Effects of Some Chemicals on the Enzyme Activity. Selcuk University Journal of Science Faculty [Internet]. 2003 Dec. 1;1(21):63-74. Available from: https://izlik.org/JA85US64AJ
