Güneydoğu Anadolu'da Yaygın Olarak Dağılış Gösteren Melanopsis praemorsa (L., 1758) Gastropoda: Prosobranchia)’nın Yağ Asiti içeriği

Abstract

Bu çalışmada, Diyarbakır Devegeçidi Köprüsü civarından, 2007 yılı haziran ayında toplanan tatlısu salyangozu Melanopsis praemorsa'nın total vücut lipitleri; ince tabaka kromatografi ile fraksiyonlandı. Salyangozun, total vücut lipitleri, fosfolipit ve nötral lipit fraksiyonundaki yağ asitleri ile besinini oluşturan tatlısu alglerinin yağ asitleri gaz kromatografi ve gaz kromatografi-kütle spektrometresi (GC-MS) ile analizlendi. Analizlerde, doymuş yağ asitlerinden C10:0, C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0; tekli doymamış yağ asitlerinden C16:1ω7, C18:1ω9, C20:1ω9 ve çoklu doymamış yağ asitlerinden C18:2ω6, C18:3ω3, C20:2ω6, C20:4ω6, C20:5ω3 ve C22:2ω6 asitler saptandı. Tek karbonlu ve C20:2ω6, C20:4ω6, C20:5ω3 ve C22:2ω6 gibi 20 karbonlu çoklu doymamış yağ asitleri, gaz kromatografi-kütle spektrometre ile doğrulandı. Lipit fraksiyonlarında bu bileşenlerin yüzde değerleri karşılaştırıldı. Yüzde dağılımda en çok C16:0, C18:1ω9 ve C18:2ω6 asitler tespit edildi. Fosfolipit, nötral, total ve besin (alg) lipitlerinin yüzde içeriğinde bazı farklar tespit edildi.

Keywords

• Yağ Asitleri, Melanopsis praemorsa, Tatlısu salyangozu, Güneydoğu Anadolu

Fatty Acid Composition of Melanopsis praemorsa (L., 1758) (Gastropoda:Prosobranchia) Distributed in the Southeast Anatolia

Abstract

In this study, body lipids of a freshwater snail Melanopsis praemorsa collected from Devegeçidi Bridge in Diyarbakır in June 2007, were fractionated by thin layer chromatography (TLC). Total lipids, fractionated lipids such as phospholipids and neutral lipids of whole snail and algae as diets were analysed by capillary gas chromatography and gas chromatography-mass spectrometry. In the analysis, saturated fatty acids such as C10:0, C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0; monounsaturated fatty acids such as C16:1ω7, C18:1ω9, C20:1ω9 and polyunsaturated fatty acids such as C18:2ω6, C18:3ω3, C20:2ω6, C20:4ω6, C20:5ω3, C22:2ω6 asids were found. Odd-numbered fatty acids and C20 polyunsaturated fatty acids such as C20:2ω6, C20:4ω6, C20:5ω3 and C22:2ω6 were confirmed by gas chromatography-mass spectrometry. The percentages of these components in lipid fractions were compared with each other. In the percentages, C16:0, C18:1ω9 and C18:2ω6 acids were the most abundant fatty acids in the freshwater snail. Some differences were found in the percentages of phospholipid, neutral, total and algae lipids.

Keywords

• Fatty acids, Melanopsis praemorsa, Freshwater snail, the Southeast Anatolia

References

1. 1. Ekman, S. Zoogeography of the Sea, 417, Plenum Pres, London, (1953).
2. 2. Khlebovich, V.V. The Critical Salinity of Biological Processes, 230, Nauka, Leningrad, (1974).
3. 3. Vernberg, W.B. and Vernberg, F.J. Environmental Physiology of Marine Animals, 346, Springer, Berlin, (1972).
4. 4. Dembitsky, V.M., Rezanka, T., Kashin, A.G. Comparative study of the endemic freshwater fauna of genus Valvata. Comparative Biochemistry and Physiology, 107B, 325-330, (1994).
5. 5. Zhukova, N.V. The pathway of the biosynthesis of non-methylene-interrupted dienoic fatty acids in molluscs. Comparative Biochemistry and Physiology, 110B, 801-804, (1991).
6. 6. Dembitsky, V.M., Kashin, A.G., Stefanow, K. Comparative investigation of phospholipids and fatty acids of freshwater molluscs from Volga River basin. Comparative Biochemistry and Physiology, 102B (1), 193-198, (1992).
7. 7. Fried, B., Rao, K.S., Sherma, J., Huffmani, J.E. Fatty acid composition of Goniobasis virginicai, Physa sp. and Viviparus malleatus (Mollusca : Gastropoda) from lake Musconetcong, New Jersey. Biochemical Systematic and Ecology, 21(8), 809-812, (1993).
8. 8. Bilgin, F.H. Studies on the functional anatomy of Melanopsis praemorsa and Zemelanopsis trifasciata gray. Proceedings of Malacological Society of London, 40, 379-399, (1973).

9. 9. Şeşen, R. Türkiye Yumuşakça faunası için yeni bir fosil kayıt: Melanopsis corrugata (Gastropoda:Prosobranchia). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 5(1), 186-190, (2001).
10. 10. Bligh, E.G. and Dyer, W.J. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917, (1959).
11. 11. Stanley-Samuelson, D.W. and Dadd, R.H. Long chain polyunsaturated fatty acids: Patterns of occurence in insects. Biochemistry, 13, 549-558, (1983).
12. 12. Dembitsky, V.M., Rezanka, T., Kashin, A.G. Comparative study of the endemic freshwater fauna of Lake Baikal-l. Phospholipids and fatty acid composition of two mollusc species, Baicalia oviformis and Benedictia baicalensis. Comparative Biochemistry and Physiology, 106B (4), 819-823, (1993b)
13. 13. Fried, B., Rao, S.K., Sherma, J. Fatty acid composition of Biomphalaria glabrata (Gastropoda: Planorbidae) fed hen’s egg yolk versus leaf lettuce. Comparative Biochemistry and Physiology, 101A, 351-352, (1992a).
14. 14. Fried, B., Rao, K.S., Sherma, J. Fatty acid composition of two strains of Helisoma trivolvis (Gastrpoda). Biochemical Systematic and Ecology, 20(6), 553-557, (1992b).
15. 15. Go, J.V., Rezanka, T., Srebnik, M., Dembitsky, V.M. Variability of fatty acid component of marine and freshwater gastropod species from the littoral zone of the Red Sea, Mediterranean Sea and Sea of Galilee. Biochemical Systematic and Ecology, 30, 819-835, (2002).
16. 16. Dembitsky,V.M., Rezanka,T. and Kashin,A.G. Fatty acid and phospholipids composition of freshwater molluscs Anadonta piscinalis and Limnaea fragilis from the River Volga. Comparative Biochemistry and Physiology, 105B, 3(4), 597-601, (1993a).
17. 17. Logue, J.A., Howell, B.R., Bell, J.G., Cossins, A.R. Diatery n-3 long-chain polyunsaturated fatty acid deprivation, tissue lipid composition, ex-vivo prostaglandin production and stress tolerance in juvenile Dover sole ( Solea solea L.). Lipids, 35, 745-755, (2000).
18. 18. Nichols, D.S., Olley, J., Garda, N., Brenner R.R. and McMeekin T.A. Effect of temperature and salinity stress on growth and lipid composition of Shewanella gelidimarina. Applied Environmental Microbiology, 66(6) 2422-2429, (2000).