Minimum Inhibition Concentration of an Antimicrobial Agent Containing Methylisothiazolinone Used in Leather Industry Against Reference Strains

Abstract

In leather industry soaking process is applied to salted hides and skins in order to rehydrate, to soften, to remove the salt, blood, dung, dirt and microorganisms. The soaking process is a favorable environment for bacterial growth, and during this process significant damage occurs on the hides and skins.  It is known that soaked hides/skins contain Gram-positive and Gram-negative bacteria, which can adversely affect hide/skin quality even though they are treated with antimicrobial agents. Insufficient and random use of antimicrobial agents is inadequate in controlling the numbers of these bacteria, and excessive use of them also leads to the emergence of antimicrobial-resistant bacteria. The choice of appropriate antimicrobial agent is very important in preventing damage caused by bacteria found on the hides. Therefore, application of methylisothiazolinone-containing antimicrobial agent used in the leather industry against reference bacterial strains and investigation of the minimum inhibitor concentration will provide important information on the effectiveness of this antimicrobial agent on bacteria that may be harmful to hides in leather processing. In the present study, the eighteen different concentration of antimicrobial agent containing methylisothiazolinone were applied against Gram-negative (Pseudomonas aeruginosa ATCC27853, Escherichia coli ATCC25922) and Gram-positive (Staphylococcus epidermidis ATCC12228, Staphylococcus aureus ATCC29213, Micrococcus luteus ATCC9341, Enterococcus faecalis ATCC29212, Bacillus cereus ATCC11778, Bacillus subtilis ATCC6633) reference strains and their mixed culture. The minimum inhibitor concentrations were determined using agar dilution method. The minimum inhibitor concentrations of antimicrobial agent were found as 5000 µg/ml for Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC29213, Micrococcus luteus ATCC9341, mixed culture of test bacteria; 1250 µg/ml for Escherichia coli ATCC25922; 156 µg/ml for Bacillus cereus ATCC11778; 39 µg/ml for Staphylococcus epidermidis ATCC12228 and Enterococcus faecalis ATCC29212; and 9.76 µg/ml for Bacillus subtilis ATCC6633. As a result, it was determined that the tested antimicrobial agent inhibited the growth of eight different reference strains and their mixed culture, and that spectrum of action was broad.

Keywords

• Antimicrobial agent,Gram-positive bacteria,Gram-negative bacteria,Minimum inhibition concentration

Deri Endüstrisinde Kullanılan Metilizotiazolinon İçeren Antimikrobiyal Maddenin Referans Suşlar Üzerine Minimum İnhibisyon Konsantrasyonu

Abstract

Deri endüstrisinde tuzlanmış deriye kaybettiği suyun geri kazandırılması, derinin yumuşatılması, derideki tuzun, kanın, gübrenin, pisliğin ve mikroorganizmaların uzaklaştırılması amacıyla ıslatma işlemi uygulanmaktadır. Islatma işlemi bakterilerin gelişmesi için oldukça uygun bir ortam olup, bu süreçte derilerde önemli hasarlar meydana gelmektedir. Islatılmış deriler antimikrobiyal maddelerle muamele edilmesine rağmen, bu derilerin kalitesini olumsuz etkileyebilecek gram-pozitif ve gram-negatif bakterileri içerdikleri bilinmektedir. Antimikrobiyal maddelerin az ve rastgele kullanılması bu bakterilerin sayılarının kontrol edilmesinde yetersiz kaldığı gibi, fazla kullanılması da antimikrobiyallere dirençli bakterilerin ortaya çıkmasına sebep olmaktadır. Uygun antimikrobiyal madde seçimi derilerde bakterilerden kaynaklanan zararın önlenmesi açısından oldukça önemlidir. Bu nedenle, deri endüstrisinde kullanılan bir antimikrobiyal maddenin referans bakteri suşlarına karşı uygulanması ve minimum inhibisyon konsantrasyonunun araştırılması, deri işlenti basamaklarında deriye zarar verebilecek bakterilerin üzerine bu antimikrobiyal maddenin etkinliğinin belirlenmesi açısından önemli bilgi verecektir. Bu çalışmada gram-negatif ve gram-pozitif referans suşları ve bunların karışık kültürü üzerine metilizotiazolinon içeren antimikrobiyal maddenin onsekiz farklı konsantrasyonu uygulanarak minimum inhibisyon konsantrasyonu agar dilüsyon metodu ile araştırılmıştır. Minimum inhibisyon konsantrasyon değerleri Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC29213, Micrococcus luteus ATCC9341, ve tüm test bakterilerinin karışık kültürü için 5000 µg/ml; Escherichia coli ATCC25922 için 1250 µg/ml; Bacillus cereus ATCC11778 için 156 µg/ml; Staphylococcus epidermidis ATCC12228 ve Enterococcus faecalis ATCC29212 için 39 µg/ml; ve Bacillus subtilis ATCC6633 için ise 9.76 µg/ml olarak bulunmuştur. Sonuç olarak, test edilen antimikrobiyal maddenin sekiz farklı referans suşun ve bunların karışık kültürünün gelişimlerini inhibe ettiği ve etki spektrumunun geniş olduğu saptanmıştır.

Keywords

• Antimikrobiyal madde,Gram-pozitif bakteri,Gram-negatif bakteri,Minimum inhibisyon konsantrasyonu

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