Teratological Effects of Tribenuron Methyl on Zebra Fish Embryo and Larva

Abstract

Tribenuron methyl is an herbicide used in agriculture to control perennial dicots. It can access aquatic ecosystems through groundwater and affect aquatic life. The zebrafish (Danio rerio Hamilton, 1822) is a vertebrate model that has an easy reproductive capacity and transparent embryos therefore frequently preferred in scientific studies. In this study, the effects of 1.812 mg L-1, 0.906 mg L-1, 0.453 mg L-1, 0.226 mg L-1 and 0.1113 mg L-1 tribenuron methyl on zebrafish embryo and larval development were investigated. As a result of this study, it was determined that tribenuron methyl administration had toxic effects on embryo cells and caused different malformations.

Keywords

• Tribenuron Methyl
• Zebrafish (Danio rerio)
• Embryo Toxicity Test
• Teratology

Tribenuron Metil’in Zebra Balığı Embriyo ve Larvaları Üzerine Teratolojik Etkileri

Abstract

Tribenuron metil tarımda çok yıllık dikotları kontrol etmek için kullanılan herbisittir. Sucul ekosistemlere yeraltı suyu yoluyla erişebilir ve sucul yaşamı etkileyebilir. Zebra balığı (Danio rerio Hamilton, 1822), kolay üreme kapasitesine ve şeffaf embriyolara sahip olan ve bu yüzden bilimsel çalışmalarda sıkça tercih edilen bir omurgalı modelidir. Bu çalışmada tribenuron metil zebra balıkları üzerinde 120 saatlik LC50 değerinin 1.850 mg L-1 olarak tespit edildi. Bu değerden yola çıkarak uygulanan 1.812 mg L-1, 0.906 mg L-1, 0.453 mg L-1, 0.226 mg L-1 ve 0.113 mg L-1 konsantrasyonlarında tribenuron metilin zebra balığı embriyo ve larva gelişimine olan etkisi incelenmiştir. Bu çalışma sonucunda tribenuron metil uygulamasının embriyo hücrelerinde toksik etki ettiği, farklı malformasyonlara sebep olduğu tespit edilmiştir.

Keywords

• Tribenuron Metil
• Zebra Balığı (Danio rerio)
• Embriyo Toksite Testi
• Teratoloji

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