Ergin ve Ergin Olmayan Gökkuşağı Alabalığı Oncorhynchus mykiss’in (Osteichthyes: Salmonidae) Karaciğer Lipaz Enzimi (E.C 3.1.1.3) Aktivitesinin Belirlenmesi
Abstract
Bu çalışmada, ergin ve ergin olmayan Oncorhynchus mykiss’ in karaciğer lipaz (E.C 3.1.1.3) enzimi aktivitesinin belirlenmesi amaçlanmıştır. Ergin ve ergin olmayan O. mykiss karaciğer lipazını saflaştırma işlemleri 3 adımdan oluşmuştur: Homojenatın hazırlanması, amonyum sülfat çöktürmesi, Diethylamino ethyl (DEAE) selülozik iyon değişim kromatografisi. Saflaştırma işlemleri boyunca, ergin balıkların karaciğerinden elde edilen lipaz enziminin spesifik aktivitesi 2.23±1.59 µmol/mg protein, verimi %0.037 ve saflaştırma katsayısı 2.65±1.94 olarak belirlenmiştir. Ergin olmayan balıklarda ise spesifik aktivite 1.86±0.14 µmol/mg protein, verim %3.24±0.51 ve saflaştırma katsayısı 15.50±1.13 bulunmuştur.
Keywords
Determination of Liver Lipase Enzyme Activity (E.C 3.1.1.3) of Mature and Immature Rainbow Trout Oncorhynchus mykiss (Osteichthyes: Salmonidae)
Abstract
In this study, determination of liver lipase activity of mature and immature Oncorhynchus mykiss has been purposed. Liver lipase (E.C 3.1.1.3) purification process from mature and immature O. mykiss consisted of three steps: Preparation of homogenate, ammonium sulfate precipitation, Diethylamino ethyl (DEAE) cellulose ion exchange chromatography. Through the purification procedure, liver lipase enzyme of mature fish was purified with a yield of 0.037± 0.06% and a purification coefficient of 2.65±1.94, having the spesific activity of 2.23±1.59 µmol/mg protein. From immature fish, the enzyme was purified with a yield of 3.24±0.51% and a purification coefficient of 15.50±1.13, having the spesific activity of 1.86±0.14 µmol/mg protein.
Keywords
Details
| Other ID | JA58CF75HB |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 1, 2009 |
| Submission Date | June 1, 2009 |
| Published in Issue | Year 2009 Volume: 2 Issue: 34 |
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Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
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