Abstract
This study was carried out to compare rapid bacterial DNA extraction methods. Strains of Staphylococcus sp. and E. coli were preferred to represent Gram positive and Gram negative bacteria, respectively. DNA extractions from the cultured bacteria (~ 108 cfu/ml) were made by the methods of boiling, sonication and freze-thawing. Then, the quantities of DNA extracted were measured by spectrophotometric analysis at 260nm. DNA quantities obtained by sonication method were statistically more than those obtained by other methods (p<0.05)
References
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- van Belkum, A. DNA Fingerprinting of Medically Important Microorganisms by Use of PCR. Clin. Microbiol. Rev. 7: 84-174 (1995).
- Wang, G., Whittam, T. S., Berg, C. M., Berg, D.E. RAPD (Arbitrary Primer) PCR is More Sensitive Than Multilocus Enzyme Electrophoresis for Distinguishing Related Bacterial Strains. Nucleic Acids Res. 21: 5930-5933 (1993).
- Kevin, A., Comparison of Rapid Methods for the Extraction of Bacterial DNA from Colonic and Fecal Lumen Contents of the Pig. J. Applied Microbiol. 94: 988-993 (2003).
- Rengarajen, K., Cristol, M.S., Mehta, M., Nickerson, J.M. Quantifying DNA Concentrations Using Fluorometry: A Comparison of Fluorophores. Molecular Vision. 8: 416-421 (2002).
- Maniatis, T., Sambrook, J. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Pres, USA. (1989).
- Newton, C.R. PCR Essential Data. John Wiley and Sons LTD. Baffins Lane, Chicheter, West Sussex PO 19, 1UD, UK. (1995).
- More, M.I., Herrick, J.B., Silva, M.C., Ghiorse, W.C., Madsen, E.L. Quantitative Cell Lysis of Indigenous Microorganism and Rapid Extraction of Microbial DNA from Sediment. Applied and Environmental Microbiology 60:5, 1572-1580 (1994).
- Yeates, C., Gillings M. R., Davison, A. D., Altavilla, N., Veal, D. A. Methods for Microbial DNA Extraction from Soil for PCR Amplification. Biological Procedures Online. 1:40-47 (1998).
- 0. Pitcher, D. G., Saunders, N. A., Owen R. J. Rapid Extraction of Bacterial Genomic DNA with Guanidium Thiocyanate. Letters in Applied Microbiology 8:151-156 (1989).
Abstract
Bu çalışma bakteriyel DNA ekstraksiyonunda kullanılan hızlı metotları karşılaştırmak amacıyla yapıldı. Bu amaçla Gram pozitif bakterilere örnek olarak Staphylococcus sp., Gram negatiflere örnek olarak Escherichia coli seçildi. Bu bakterilerin buyyon kültürlerinden (~107 cfu/ml) kaynatma, sonikasyon ve dondurma-çözme yöntemleri ile DNA ekstraksiyonu yapıldı. DNA miktarları 260 nm dalga boyunda spektrofotometre ile okundu. Sonikasyon ile elde edilen DNA miktarı diğer yöntemlerle elde edilen DNA miktarlarından istatistiksel olarak daha fazla bulundu (p
References
- Maslow, J. N., Mulligan, M. E., Arbeit, R. D. Molecular Epidemiology: Application of Contemporary Techniques to the Typing of Microorganisms. Clin. Infect Dis. 17: 64-153 (1993).
- van Belkum, A. DNA Fingerprinting of Medically Important Microorganisms by Use of PCR. Clin. Microbiol. Rev. 7: 84-174 (1995).
- Wang, G., Whittam, T. S., Berg, C. M., Berg, D.E. RAPD (Arbitrary Primer) PCR is More Sensitive Than Multilocus Enzyme Electrophoresis for Distinguishing Related Bacterial Strains. Nucleic Acids Res. 21: 5930-5933 (1993).
- Kevin, A., Comparison of Rapid Methods for the Extraction of Bacterial DNA from Colonic and Fecal Lumen Contents of the Pig. J. Applied Microbiol. 94: 988-993 (2003).
- Rengarajen, K., Cristol, M.S., Mehta, M., Nickerson, J.M. Quantifying DNA Concentrations Using Fluorometry: A Comparison of Fluorophores. Molecular Vision. 8: 416-421 (2002).
- Maniatis, T., Sambrook, J. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Pres, USA. (1989).
- Newton, C.R. PCR Essential Data. John Wiley and Sons LTD. Baffins Lane, Chicheter, West Sussex PO 19, 1UD, UK. (1995).
- More, M.I., Herrick, J.B., Silva, M.C., Ghiorse, W.C., Madsen, E.L. Quantitative Cell Lysis of Indigenous Microorganism and Rapid Extraction of Microbial DNA from Sediment. Applied and Environmental Microbiology 60:5, 1572-1580 (1994).
- Yeates, C., Gillings M. R., Davison, A. D., Altavilla, N., Veal, D. A. Methods for Microbial DNA Extraction from Soil for PCR Amplification. Biological Procedures Online. 1:40-47 (1998).
- 0. Pitcher, D. G., Saunders, N. A., Owen R. J. Rapid Extraction of Bacterial Genomic DNA with Guanidium Thiocyanate. Letters in Applied Microbiology 8:151-156 (1989).
Details
| Other ID | JA58JG75GM |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 1, 2006 |
| Submission Date | December 1, 2006 |
| Published in Issue | Year 2006 Volume: 2 Issue: 27 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
It has been scanned in DergiPark since 2016.
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