Sideritis hololeuca Boiss. & Heldr. apud Bentham ve Sideritis libanotica Labill. subsp. violascens (P.H.Davis) P.H.Davis Ekstrelerinin Antibakteriyel Aktivitelerinin Belirlenmesi

Year 2009, Volume: 2 Issue: 33, 29 - 36, 01.12.2009

Ayça Ayaz Rüstem Duman

Abstract

Türkiye’de endemik olarak bulunan Sideritis hololeuca Boiss. & Heldr. apud Bentham ve Sideritis libanotica Labill. subsp. violascens (P.H.Davis) P.H.Davis bitki türlerinin toprak üstü kısımlarından elde edilen kloroform, aseton ve etanol ekstreleri, 8 adet Gram-pozitif (Bacillus cereus ATCC 14579, Bacillus subtilis B RSHMB, Listeria monocytogenes tip 2 NCTC 5348, Micrococcus luteus LA 2971, Staphylococcus aureus ATCC 29740, Streptococcus mutans NCTC 10449, Streptococcus pneumoniae ATCC 6305 ve Streptococcus pyogenes ATCC 19615) ve 6 adet Gram-negatif (Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 10031, Proteus mirabilis ATCC 15146, Proteus vulgaris ATCC 7829, Pseudomonas aeruginosa ATCC 15442 ve Salmonella enteritidis RSHMB) olmak üzere toplam 14 adet standart bakteri suşuna karşı disk difüzyon metoduyla araştırıldı. Araştırma sonucunda; Bacillus cereus, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa ve Salmonella enteritidis’e karşı hiçbir ekstrenin antibakteriyel aktiviteye sahip olmadığı, ancak zayıf antibakteriyel aktiviteye sahip olan ekstrelerden en az birinin diğer bakteri türlerine karşı etkili olduğu bulundu.

Keywords

Sideritis hololeuca, Sideritis libanotica subsp. violascens, antibakteriyel aktivite, disk difüzyon metodu

Determination of Antibacterial Activities of Sideritis hololeuca Boiss. & Heldr. apud Bentham and Sideritis libanotica subsp. violascens (P.H.Davis) P.H.Davis Extracts

Abstract

The antibacterial activity of the chloroform, acetone and ethanol extracts obtained from the aerial parts of Sideritis hololeuca Boiss.&Heldr. apud Bentham and Sideritis libanotica subsp. violascens (P.H.Davis) P.H.Davis (endemic species) were investigated using the disc diffusion method against 14 different bacteria [8 Gram positive (Bacillus cereus ATCC 14579, Bacillus subtilis B RSHMB, Listeria monocytogenes tip 2 NCTC 5348, Micrococcus luteus LA 2971, Staphylococcus aureus ATCC 29740, Streptococcus mutans NCTC 10449, Streptococcus pneumoniae ATCC 6305 and Streptococcus pyogenes ATCC 19615) and 6 Gram negative (Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC 10031, Proteus mirabilis ATCC 15146, Proteus vulgaris ATCC 7829, Pseudomonas aeruginosa ATCC 15442 and Salmonella enteritidis RSHMB)]. By the results of the study it was determined that no extracts showed any antibacterial activities against the bacteriae Bacillus cereus, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Salmonella enteritidis,while at least one of the extracts which showed antibacterial activity weakly was found to be effective against others of the bacteriae tested.

Keywords

Sideritis hololeuca, Sideritis libanotica subsp. violascens, antibacterial activity, disc diffusion method

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