Örümceklerde (Ordo: Araneae) farklı muhafaza koşullarının DNA Sekanslama ve Barkotlama Analizleri Üzerine Etkileri
Abstract
Bu
çalışmada farklı koşullarda muhafaza edilen Lycosidae ve Thomisidae (Ordo: Araneae) familyalarına ait müze
örneklerinden DNA izole edilmiş ve DNA barkotlama çalışmaları yapılmıştır. Örümceklerin vücut büyüklüğü, farklı alkol
değerleri ve DNA konsantrasyonlarının DNA sekansları üzerine etkisi
araştırılmıştır. 15 yıllık örneklerden genomik DNA izolasyonları yapılmış, iki
farklı gen bölgesi (COI, ITS) PCR ile çoğaltılmış ve sekanslama işlemlerine
tabi tutulmuştur. Sekanslama üzerine örneklerde vücut büyüklüğü ile DNA
konsantrasyonu arasında bir ilişki belirlenemezken, saklama koşullarının DNA
kalitesini ve sekanslamayı etkilediği belirlenmiştir.
Keywords
References
- Kaynaklar A’Haea, S., Harling R, McKinlay RG, Topping CJ(1998). RAPD profiling of spider (Araneae) DNA. Journal of Arachnology. 26: 397-400.
- Anderson JC, Mills NJ(2012). DNA extraction from museum specimens of parasitic Hymenoptera. PloS ONE 7:e45549.
- Barret RDH, Hebert PDN (2005). Identifying spiders through DNA barcodes. Canadian Journal of Zoology.83: 481-491.
- Bayram, A., Kunt, K.B. and Danışman, T. (2017). The Checklist of the Spiders of Turkey. Version 2017, Online at http://www.spidersofturkey.info
- Dayrat B (2005). towards integrative taxonomy. Biological Journal of the Linnean Society.85:407-415.
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994).DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol.3(5):294-9.
- Goldstein PZ, DeSalle R (2011). Integrating DNA barcode data and taxonomic practise: Determination, discovery and description. Bioessays. 33:135-147.
- Ji, Y.-J., Zhang, D.-H.&He,L,-J. (2003). Rvolutionary conservation and versatility of a new set of primers amplifying internal transcribed spacer regions in insect and other invertabrates.Molecular Ecology Notes.3:581-585.
- Nentwig W, Blick T, Gloor D, Hänggi A, Kropf C (2017). Spiders of Europe. www.araneae.unibe.ch. Version of access date : 10.2017
- Qıagen.(2006). DNeasy Blood & Tissue Handbook.(USA).
- Vink CJ, Thomas SM, Paquin P, Hayashi CY, Hedin M (2005).The effect of preservatives and temparatures on Arachnid DNA. İnvertebrate Systematics.19:99-104.
- Will KW, Mishler BD, Wheeler QD (2005). The perils of DNA barcoding and the need for integrative taxonomy. Systematic Biology. 54:844-851.
- Zuccon D, Brisset j, Corbari L, Puillandre N, Utge J, Samadi S (2012). An optimised protocol for barcoding museum collections of decapod crustaceans: a case-study for a 10-40 years- old collection. Invertebrate Systematics.26:592-600.
Abstract
References
- Kaynaklar A’Haea, S., Harling R, McKinlay RG, Topping CJ(1998). RAPD profiling of spider (Araneae) DNA. Journal of Arachnology. 26: 397-400.
- Anderson JC, Mills NJ(2012). DNA extraction from museum specimens of parasitic Hymenoptera. PloS ONE 7:e45549.
- Barret RDH, Hebert PDN (2005). Identifying spiders through DNA barcodes. Canadian Journal of Zoology.83: 481-491.
- Bayram, A., Kunt, K.B. and Danışman, T. (2017). The Checklist of the Spiders of Turkey. Version 2017, Online at http://www.spidersofturkey.info
- Dayrat B (2005). towards integrative taxonomy. Biological Journal of the Linnean Society.85:407-415.
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994).DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol.3(5):294-9.
- Goldstein PZ, DeSalle R (2011). Integrating DNA barcode data and taxonomic practise: Determination, discovery and description. Bioessays. 33:135-147.
- Ji, Y.-J., Zhang, D.-H.&He,L,-J. (2003). Rvolutionary conservation and versatility of a new set of primers amplifying internal transcribed spacer regions in insect and other invertabrates.Molecular Ecology Notes.3:581-585.
- Nentwig W, Blick T, Gloor D, Hänggi A, Kropf C (2017). Spiders of Europe. www.araneae.unibe.ch. Version of access date : 10.2017
- Qıagen.(2006). DNeasy Blood & Tissue Handbook.(USA).
- Vink CJ, Thomas SM, Paquin P, Hayashi CY, Hedin M (2005).The effect of preservatives and temparatures on Arachnid DNA. İnvertebrate Systematics.19:99-104.
- Will KW, Mishler BD, Wheeler QD (2005). The perils of DNA barcoding and the need for integrative taxonomy. Systematic Biology. 54:844-851.
- Zuccon D, Brisset j, Corbari L, Puillandre N, Utge J, Samadi S (2012). An optimised protocol for barcoding museum collections of decapod crustaceans: a case-study for a 10-40 years- old collection. Invertebrate Systematics.26:592-600.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | April 30, 2018 |
| Submission Date | October 30, 2017 |
| Published in Issue | Year 2018 Volume: 44 Issue: 1 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
It has been scanned in DergiPark since 2016.
Selcuk University Journal of Science Faculty is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.