Yumurtaya Verilen Siklofosfamid ve C Vitamini’nin Tavuk Embriyoları Üzerindeki Bazı Etkileri

Year 2018, Volume: 44 Issue: 2, 157 - 173, 11.10.2018

Haluk Özparlak Bülent Çelik Döndü Balta

Abstract

Literatürdeki
in vivo ve in vitro ortamda gerçekleştirilen pek çok
genotoksisite-antigenotoksisite çalışmasında genotoksik bir madde olarak
Siklofosfamid (CP) ve antigenotoksik bir madde olarak C vitamini (Askorbik
Asit, AA) yaygın olarak kullanılmaktadır. Bunlar alternatif bir mikronükleus
testi olan tavuk yumurtası mikronükleus testinde (Hen’s Egg Test for Analysis
of Micronucleus Induction, HET-MN) de 50 µg/yumurta (50 µg/y) dozunda
kullanılmaktadır. Bu çalışmayla 50 µg/y dozunda CP ve AA’nın tavuk embriyoları
üzerindeki bazı etkilerinin ilk kez tespit edilmesi amaçlandı. Çalışma için
döllü tavuk yumurtalarından oluşan dört grup oluşturuldu. CP, AA, CP+AA
birlikte ve steril bidistile su (kontrol olarak) gruplardaki yumurtaların hava
kamarasına kuluçkanın 8. gününde enjekte edildi. Kuluçkanın 11. gününde her bir
grubun ölü ve anormal embriyo oranları, malformasyon tipleri, canlı ve rölatif
embriyo oranları tespit edildi. Ayrıca kemik gelişiminin belirlenebilmesi için
embriyoların bir kısmı total olarak Alizarin Red-S yöntemiyle boyandı. Çalışma
iki tekrarlı olarak gerçekleştirildi. İki denemenin sonucunun birbiriyle uyumlu
olduğu tespit edildi.  Sonuç olarak, test
edilen dozda CP, AA ve CP+AA’nın önemli bir embriyotoksik veya teratojenik etki
göstermediği ve embriyoların kemik gelişimini makroskobik düzeyde etkilemediği
belirlendi. 

Keywords

Alizarin Red-S, Askorbik asit, Siklofosfamid, Tavuk Embriyosu

Some Effects of Cyclophosphamide and Vitamin C Given in ovo on Chicken Embryos

Abstract

In the
literature, Cyclophosphamide (CP) as a genotoxic substance and vitamin C
(Ascorbic Acid, AA) as an antigenotoxic substance have been used extensively in
many genotoxicity-antigenotoxicity studies conducted in vivo and in vitro, and
these substances at doses of 50 µg/egg are also used in Hen’s Egg Test for
Analysis of Micronucleus Induction (HET-MN) which is an alternative
micronucleus test. The aim of this study was to determine the some effects of
CP and AA at 50 µg/egg dose on the development of chicken embryos for the first
time. Four groups were formed from the fertilized hen's eggs. CP, AA and CP+AA
together, and sterile bidistilled water (as a control) were injected into the
air sac of eggs in groups at 8th day of incubation. Following parameters of
each group were examined on 11th day of the incubation: rates of dead and
abnormal embryo, malformation types, live and relative embryo weights. In
addition, some of the embryos were totally stained with Alizarin Red-S method
for bone development. Two trials were conducted in the study. The results of
the two trials were compatible with each other. As a result, CP, AA and CP+AA
together at the examined doses did not present significant embryotoxic and
teratogenic effects on chick embryos. They also did not affect the bone
development of chicken embryos at the macroscopic level.

Keywords

Alizarin Red-S, Ascorbic Acid, Cyclophosphamide, Chick Embryo

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