Yumurtaya Verilen Siklofosfamid ve C Vitamini’nin Tavuk Embriyoları Üzerindeki Bazı Etkileri

Year 2018, Volume 44, Issue 2, 157 - 173, 11.10.2018

Haluk ÖZPARLAK Bülent ÇELİK Döndü BALTA

Abstract

Literatürdeki in vivo ve in vitro ortamda gerçekleştirilen pek çok genotoksisite-antigenotoksisite çalışmasında genotoksik bir madde olarak Siklofosfamid (CP) ve antigenotoksik bir madde olarak C vitamini (Askorbik Asit, AA) yaygın olarak kullanılmaktadır. Bunlar alternatif bir mikronükleus testi olan tavuk yumurtası mikronükleus testinde (Hen’s Egg Test for Analysis of Micronucleus Induction, HET-MN) de 50 µg/yumurta (50 µg/y) dozunda kullanılmaktadır. Bu çalışmayla 50 µg/y dozunda CP ve AA’nın tavuk embriyoları üzerindeki bazı etkilerinin ilk kez tespit edilmesi amaçlandı. Çalışma için döllü tavuk yumurtalarından oluşan dört grup oluşturuldu. CP, AA, CP+AA birlikte ve steril bidistile su (kontrol olarak) gruplardaki yumurtaların hava kamarasına kuluçkanın 8. gününde enjekte edildi. Kuluçkanın 11. gününde her bir grubun ölü ve anormal embriyo oranları, malformasyon tipleri, canlı ve rölatif embriyo oranları tespit edildi. Ayrıca kemik gelişiminin belirlenebilmesi için embriyoların bir kısmı total olarak Alizarin Red-S yöntemiyle boyandı. Çalışma iki tekrarlı olarak gerçekleştirildi. İki denemenin sonucunun birbiriyle uyumlu olduğu tespit edildi.  Sonuç olarak, test edilen dozda CP, AA ve CP+AA’nın önemli bir embriyotoksik veya teratojenik etki göstermediği ve embriyoların kemik gelişimini makroskobik düzeyde etkilemediği belirlendi. 

Keywords

Alizarin Red-S, Askorbik asit, Siklofosfamid, Tavuk Embriyosu

Some Effects of Cyclophosphamide and Vitamin C Given in ovo on Chicken Embryos

Abstract

In the literature, Cyclophosphamide (CP) as a genotoxic substance and vitamin C (Ascorbic Acid, AA) as an antigenotoxic substance have been used extensively in many genotoxicity-antigenotoxicity studies conducted in vivo and in vitro, and these substances at doses of 50 µg/egg are also used in Hen’s Egg Test for Analysis of Micronucleus Induction (HET-MN) which is an alternative micronucleus test. The aim of this study was to determine the some effects of CP and AA at 50 µg/egg dose on the development of chicken embryos for the first time. Four groups were formed from the fertilized hen's eggs. CP, AA and CP+AA together, and sterile bidistilled water (as a control) were injected into the air sac of eggs in groups at 8th day of incubation. Following parameters of each group were examined on 11th day of the incubation: rates of dead and abnormal embryo, malformation types, live and relative embryo weights. In addition, some of the embryos were totally stained with Alizarin Red-S method for bone development. Two trials were conducted in the study. The results of the two trials were compatible with each other. As a result, CP, AA and CP+AA together at the examined doses did not present significant embryotoxic and teratogenic effects on chick embryos. They also did not affect the bone development of chicken embryos at the macroscopic level.

Keywords

Alizarin Red-S, Ascorbic Acid, Cyclophosphamide, Chick Embryo

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