Minimum Inhibition Concentration of an Antimicrobial Agent Containing Methylisothiazolinone Used in Leather Industry Against Reference Strains

Year 2018, Volume: 44 Issue: 2, 149 - 156, 11.10.2018

Pınar Çağlayan

Abstract

In leather
industry soaking process is applied to salted hides and skins in order to
rehydrate, to soften, to remove the salt, blood, dung, dirt and microorganisms.
The soaking process is a favorable environment for bacterial growth, and during
this process significant damage occurs on the hides and skins.  It is known
that soaked hides/skins contain Gram-positive and Gram-negative bacteria, which
can adversely affect hide/skin quality even though they are treated with
antimicrobial agents. Insufficient and random use of antimicrobial agents is
inadequate in controlling the numbers of these bacteria, and excessive use of
them also leads to the emergence of antimicrobial-resistant bacteria. The
choice of appropriate antimicrobial agent is very important in preventing
damage caused by bacteria found on the hides. Therefore, application of
methylisothiazolinone-containing antimicrobial agent used in the leather
industry against reference bacterial strains and investigation of the minimum
inhibitor concentration will provide important information on the effectiveness
of this antimicrobial agent on bacteria that may be harmful to hides in leather
processing. In the present study, the eighteen different concentration
of antimicrobial agent containing methylisothiazolinone
were applied against Gram-negative (Pseudomonas
aeruginosa ATCC27853, Escherichia
coli ATCC25922) and Gram-positive (Staphylococcus
epidermidis ATCC12228, Staphylococcus
aureus ATCC29213, Micrococcus luteus ATCC9341,
Enterococcus faecalis ATCC29212, Bacillus cereus ATCC11778, Bacillus subtilis ATCC6633) reference
strains and their mixed culture. The minimum inhibitor concentrations were
determined using agar dilution method. The minimum inhibitor concentrations of
antimicrobial agent were found as 5000 µg/ml for Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC29213, Micrococcus luteus ATCC9341, mixed culture of test bacteria; 1250
µg/ml for Escherichia coli ATCC25922;
156 µg/ml for Bacillus cereus ATCC11778;
39 µg/ml for Staphylococcus epidermidis ATCC12228
and Enterococcus faecalis ATCC29212;
and 9.76 µg/ml for Bacillus subtilis ATCC6633.
As a result, it was determined that the tested antimicrobial agent inhibited
the growth of eight different reference strains and their mixed culture, and
that spectrum of action was broad.

Keywords

Antimicrobial agent, Gram-positive bacteria, Gram-negative bacteria, Minimum inhibition concentration

Deri Endüstrisinde Kullanılan Metilizotiazolinon İçeren Antimikrobiyal Maddenin Referans Suşlar Üzerine Minimum İnhibisyon Konsantrasyonu

Abstract

Deri endüstrisinde tuzlanmış deriye kaybettiği suyun geri
kazandırılması, derinin yumuşatılması, derideki tuzun, kanın, gübrenin,
pisliğin ve mikroorganizmaların uzaklaştırılması amacıyla ıslatma işlemi
uygulanmaktadır. Islatma işlemi bakterilerin gelişmesi için oldukça uygun bir
ortam olup, bu süreçte derilerde önemli hasarlar meydana gelmektedir. Islatılmış deriler antimikrobiyal maddelerle muamele
edilmesine rağmen, bu derilerin kalitesini olumsuz etkileyebilecek gram-pozitif
ve gram-negatif bakterileri içerdikleri bilinmektedir. Antimikrobiyal
maddelerin az ve rastgele kullanılması bu bakterilerin sayılarının kontrol
edilmesinde yetersiz kaldığı gibi, fazla kullanılması da antimikrobiyallere
dirençli bakterilerin ortaya çıkmasına sebep olmaktadır. Uygun antimikrobiyal madde seçimi derilerde
bakterilerden kaynaklanan zararın önlenmesi açısından oldukça önemlidir. Bu nedenle, deri endüstrisinde kullanılan bir
antimikrobiyal maddenin referans bakteri suşlarına karşı uygulanması ve minimum
inhibisyon konsantrasyonunun araştırılması, deri işlenti basamaklarında deriye
zarar verebilecek bakterilerin üzerine bu antimikrobiyal maddenin etkinliğinin
belirlenmesi açısından önemli bilgi verecektir. Bu çalışmada gram-negatif ve gram-pozitif
referans suşları ve bunların karışık kültürü üzerine metilizotiazolinon içeren
antimikrobiyal maddenin onsekiz farklı konsantrasyonu uygulanarak minimum inhibisyon konsantrasyonu agar dilüsyon metodu
ile araştırılmıştır. Minimum inhibisyon
konsantrasyon değerleri
Pseudomonas aeruginosa ATCC27853, Staphylococcus
aureus ATCC29213, Micrococcus luteus ATCC9341,
ve tüm test bakterilerinin karışık kültürü için 5000 µg/ml; Escherichia coli ATCC25922 için 1250
µg/ml; Bacillus cereus ATCC11778 için
156 µg/ml; Staphylococcus epidermidis ATCC12228
ve Enterococcus faecalis ATCC29212
için 39 µg/ml; ve Bacillus subtilis ATCC6633
için ise 9.76 µg/ml olarak bulunmuştur. Sonuç olarak, test edilen
antimikrobiyal maddenin sekiz farklı referans suşun ve bunların karışık
kültürünün gelişimlerini inhibe ettiği ve etki spektrumunun geniş olduğu
saptanmıştır.

Keywords

Antimikrobiyal madde, Gram-pozitif bakteri, Gram-negatif bakteri, Minimum inhibisyon konsantrasyonu

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