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Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture

Year 2011, Volume: 20 Issue: 1, 15 - 21, 01.03.2011

Ayten Salantur Selami Yazar , Emin Dönmez , Taner Akar

Abstract

This study was carried out to determine plant regeneration response of some winter bread wheat F2 population under anther culture conditions. This research was conducted in 2008-2009 cultivation season in Central Research for Field Crops’ Biotechnology Laboratory and 21 F2 populations was used as plant material. Callus + embriyoid number, albino plant number, green plant number, sterile plant number, doubling index and success (final success) index per 100 anther criteria were examined in this study. Results showed that 15 populations produced DH plants while 4 populations produced only callus and 2 populations negatively responded to anther culture. Response of each F2 bread wheat population to DH plant regeneration was completely different based on preliminary data. F2-804, 1025, 1037 and 863 population more positively responded than others to anther culture. Seeds of the 15 DH populations were planted into field in order to use in wheat breeding program.

Keywords

Bread wheat; anther culture; plant regeneration

References

  • Ahmet H. ve M.S. Adak, 2007. Irak’ta yetiştirilen bazı ekmeklik buğday çeşitlerinde kallus oluşumu ve bitki rejenerasyonu. Tarım Bilimleri Dergisi, 13(3): 285-291.
  • Amstrong T.A., S.G. Metz and P.N. Mascia, 1987. Two regeneration systems for the production of plants from wheat anther culture. Plant Science, 51: 231-237.
  • Anonim 2005. http://www.scri.ac.uk/assoc/COST 851/DHTable2005.xls (erişim tarihi 24/03/2011).
  • Anonim 2010. Tarım İstatistikleri Özeti 1988-2009. T.C. Başbakanlık Türkiye İstatistik Kurumu.
  • Anonim 2011. http://www. wheat cap. org.
  • Ascough G.D., F. Bakos, E. Balazs, B. Barbanas and J. VanStaden, 2006. Screening South African wheat germplasm for androgenic competence. South African Journal of Botany, 72: 40-45.
  • Barbanas E., E. Szakacs, I. Karsai and Z. Bedö, 2001. In vitro androgenesis of wheat: from Fundamentals to practical application. Euphtytica, 119: 211-216.
  • Barbanas B. 2003. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.). Doubled Haploid Production in Crop Plants, 65-70.
  • Cakir M., R. Appels, M. Carter, R. Loughman, M. Francki C. Li, J. Johnson, M Bhave, R. Wilson, R. McLean and I. Barley, 2003. Accellerated wheat breeding using molecular markers. In: Pogna N (ed) X Int. Wheat Genet. Symp. Paestum Italy. Instituto Sperimentale per la Cerealicoltura. Roma-Italy. 1: 117-120.
  • De Buyser J., Y. Henry, P. Lonet, R. Hertzog and A. Hepsel. 1987. Florin: A doubled haploid wheat variety developed by the anther culture method. Plant Breeding, 98:53-56. Enginözü M. 2006. Donor bitkilerin yetişme
  • koşulları ve farklı kültür sıcaklıklarının ekmeklik buğday (Triticum aestivum L.) anter kültüründe haploid bitki
  • rejenerasyonuna etkileri üzerine bir araştırma. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Anabilim Dalı. Yüksek Lisans Tezi.
  • Hansen N.J.P. and S.B. Andersen, 1998. In vitro chromosome doubling with colchcine during microspore culture in wheat (Triticum aestivum L.). Euphytica, 102: 101-108.
  • Hatipoğlu R., İ. Atış ve S. Altıntaş, 2005. Buğday (Triticum aestivum L.) anter kültüründe genotip donor bitki yetişme koşulları ovari ile birlikte kültür ve anterlerin ortama yerleştirilme pozisyonunun haploid bitki rejenerasyonuna etkisi üzerine bir araştırma. Türkiye VI. Tarla Bitkileri Kongresi, 5-9 Eylül, Cilt II: 659-664.
  • Holme I.B., A. Olesen, N.J.P. Hansen and S.B. Andersen, 1999. Anther and isolated microspore culture response of wheat lines from nortwestern and eastern Europe. Plant Breeding, 118:111-117.
  • Hu D., Y. Tang, Z. Yuan and J. Wang, 1983. The induction of pollen sporophyt of winter wheat and development of new variety Jinghua no 1. Science Agricultural Sinica, 1:29-35.
  • Hu Y., RR. Bao and XY. Xue, 1988. The new strain ‘764’ of spring wheat by pollen haploid technique from anther culture. Genetic Manipulation in Crops Newsletter, 4:70-85.
  • Inagaki M.N. 2003. Doubled haploid production in wheat through wide hybridization. Doubled Haploid Production in Crop Plants, 53-58.
  • Löschenberger F.E. and Heberle-Bors, 1992. Anther culture responsiveness of Austrian winter wheat (Triticum aestivum L.) cultivars. Die Bodenkultur, 43: 115–122.
  • Orshinsky B.R. and R.S. Sadasivaiah, 1994. Effect of media on embryoid induction and plant regeneration from cultured anthers of soft white spring wheats (Triticum aestivum L.). Plant Science, 102:99-107.
  • Pauk J., Z. Kertesz, B. Beke, L. Bona, M. Csösz and J. Matuz, 1995. New winter wheat variety: ‘GK Delibab’ developed via combining conventional breeding and invitro androgenesis. Cer. Res. Com., 23(3):251-256.
  • Pauk J., R. Mihaly and M. Puolimatka, 2003. Protocol for wheat (Triticum aestivum L.) anther culture. Doubled Haploid Production in Crop Plants, 59-64.
  • Rahman M.M., A.K.M. Shamsuddin and U. Asad, 2008. In vitro regeneration from mature embryosis in spring wheat. Int. J. Susutain. Crop Product., 3(2):76-80.
  • Redha A., T. Attia, B. Büter, S. Saisingtong, P. Stamp and J.E. Schmid, 1998. Improved production of doubled haploids by colchcine application to wheat (Triticum aestivum L.) anther culture. Plant Cell Tissue and Organ
  • Culture, 63: 167-172.
  • Redha A., S.M.S. Islam, B. Büter, P. Stamp and J.E. Schmid, 2000. The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer. Plant Cell Tissue and Organ Culture, 63: 167-172.
  • Shahinul Islam S.M. 2010. The effect of colchcine pretreatment on isolated microspore culture of wheat. Australian Journal of Crop Science, 4 (8): 660-665.
  • Szakacs E., G. Kovacs, J. Pauk and B. Barbanas, 1989. Substitution analysis of callus induction and plant regeneration from anter culture in wheat (Triticum aestivum L.) anters. Plant Cell Rep., 7: 218-222.
  • Tuvesson S., A. Ljungberg, N. Johansson, K.E. Karlson, L.W. Suıjs and J.P. Josset, 2000. Large-scale production of wheat and triticale double haploids through the use of a singleanther culture method. Plant Breeding,
  • :455-459.
  • Zamani I., E. Gouli-Vavdinoudi, G. Kovacs, I. Xynıas, D. Roupakias and B. Barbanas, 2003. Plant Breeding, 122: 314-317.
  • Zhou H. and C.F. Konzak, 1989. Improvement of anther culture methods for haploid production in wheat. Crop Science, 29: 817-822.

Kışlık Ekmeklik Buğday F2 Popülasyonlarının Anter Kültüründe Bitki Rejenarasyonuna Tepkisinin Belirlenmesi

Year 2011, Volume: 20 Issue: 1, 15 - 21, 01.03.2011

Ayten Salantur Selami Yazar , Emin Dönmez , Taner Akar

Abstract

Bu araştırmada bazı kışlık ekmeklik buğday F2 popülasyonlarının anter kültüründe bitki rejenerasyonuna tepkisinin belirlenmesi amaçlanmıştır. Araştırma 2008-2009 yetiştirme sezonunda Tarla Bitkileri Merkez Araştırma Enstitüsü’nün Biyoteknoloji laboratuarında 21 adet F2 popülasyonu kullanılarak yürütülmüştür. Araştırmada 100 anter başına kallus+embriyoid sayısı, albino bitki sayısı, yeşil bitki sayısı, steril bitki sayısı, katlanma indeksi ve başarı indeksi incelenmiştir. Araştırma sonuçlarına göre kullanılan 15 popülasyondan katlanmış haploid bitki elde edilmiş, 4 popülasyonda sadece kallus oluşumu gözlenmiş; 2 popülasyon ise anter kültürüne hiçbir tepki göstermemiştir. Islah materyalinde her bir F2 popülasyonlarının buğday anter kültüründe haploid bitki rejenerasyonuna tepkisi birbirlerinden oldukça farklı bulunmuştur. F2-804, F2-1025, F2-1037 ve F2-863 popülasyonların anter kültürüne çok daha iyi tepki verdiği tespit edilmiştir. 15 popülasyondan elde edilen katlanmış haploid bitkilerin tohumları ıslah programında

kullanılmak amacıyla tarlaya ekilmiştir.

Keywords

Ekmeklik buğday; anter kültürü; bitki rejenerasyonu

References

  • Ahmet H. ve M.S. Adak, 2007. Irak’ta yetiştirilen bazı ekmeklik buğday çeşitlerinde kallus oluşumu ve bitki rejenerasyonu. Tarım Bilimleri Dergisi, 13(3): 285-291.
  • Amstrong T.A., S.G. Metz and P.N. Mascia, 1987. Two regeneration systems for the production of plants from wheat anther culture. Plant Science, 51: 231-237.
  • Anonim 2005. http://www.scri.ac.uk/assoc/COST 851/DHTable2005.xls (erişim tarihi 24/03/2011).
  • Anonim 2010. Tarım İstatistikleri Özeti 1988-2009. T.C. Başbakanlık Türkiye İstatistik Kurumu.
  • Anonim 2011. http://www. wheat cap. org.
  • Ascough G.D., F. Bakos, E. Balazs, B. Barbanas and J. VanStaden, 2006. Screening South African wheat germplasm for androgenic competence. South African Journal of Botany, 72: 40-45.
  • Barbanas E., E. Szakacs, I. Karsai and Z. Bedö, 2001. In vitro androgenesis of wheat: from Fundamentals to practical application. Euphtytica, 119: 211-216.
  • Barbanas B. 2003. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.). Doubled Haploid Production in Crop Plants, 65-70.
  • Cakir M., R. Appels, M. Carter, R. Loughman, M. Francki C. Li, J. Johnson, M Bhave, R. Wilson, R. McLean and I. Barley, 2003. Accellerated wheat breeding using molecular markers. In: Pogna N (ed) X Int. Wheat Genet. Symp. Paestum Italy. Instituto Sperimentale per la Cerealicoltura. Roma-Italy. 1: 117-120.
  • De Buyser J., Y. Henry, P. Lonet, R. Hertzog and A. Hepsel. 1987. Florin: A doubled haploid wheat variety developed by the anther culture method. Plant Breeding, 98:53-56. Enginözü M. 2006. Donor bitkilerin yetişme
  • koşulları ve farklı kültür sıcaklıklarının ekmeklik buğday (Triticum aestivum L.) anter kültüründe haploid bitki
  • rejenerasyonuna etkileri üzerine bir araştırma. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Anabilim Dalı. Yüksek Lisans Tezi.
  • Hansen N.J.P. and S.B. Andersen, 1998. In vitro chromosome doubling with colchcine during microspore culture in wheat (Triticum aestivum L.). Euphytica, 102: 101-108.
  • Hatipoğlu R., İ. Atış ve S. Altıntaş, 2005. Buğday (Triticum aestivum L.) anter kültüründe genotip donor bitki yetişme koşulları ovari ile birlikte kültür ve anterlerin ortama yerleştirilme pozisyonunun haploid bitki rejenerasyonuna etkisi üzerine bir araştırma. Türkiye VI. Tarla Bitkileri Kongresi, 5-9 Eylül, Cilt II: 659-664.
  • Holme I.B., A. Olesen, N.J.P. Hansen and S.B. Andersen, 1999. Anther and isolated microspore culture response of wheat lines from nortwestern and eastern Europe. Plant Breeding, 118:111-117.
  • Hu D., Y. Tang, Z. Yuan and J. Wang, 1983. The induction of pollen sporophyt of winter wheat and development of new variety Jinghua no 1. Science Agricultural Sinica, 1:29-35.
  • Hu Y., RR. Bao and XY. Xue, 1988. The new strain ‘764’ of spring wheat by pollen haploid technique from anther culture. Genetic Manipulation in Crops Newsletter, 4:70-85.
  • Inagaki M.N. 2003. Doubled haploid production in wheat through wide hybridization. Doubled Haploid Production in Crop Plants, 53-58.
  • Löschenberger F.E. and Heberle-Bors, 1992. Anther culture responsiveness of Austrian winter wheat (Triticum aestivum L.) cultivars. Die Bodenkultur, 43: 115–122.
  • Orshinsky B.R. and R.S. Sadasivaiah, 1994. Effect of media on embryoid induction and plant regeneration from cultured anthers of soft white spring wheats (Triticum aestivum L.). Plant Science, 102:99-107.
  • Pauk J., Z. Kertesz, B. Beke, L. Bona, M. Csösz and J. Matuz, 1995. New winter wheat variety: ‘GK Delibab’ developed via combining conventional breeding and invitro androgenesis. Cer. Res. Com., 23(3):251-256.
  • Pauk J., R. Mihaly and M. Puolimatka, 2003. Protocol for wheat (Triticum aestivum L.) anther culture. Doubled Haploid Production in Crop Plants, 59-64.
  • Rahman M.M., A.K.M. Shamsuddin and U. Asad, 2008. In vitro regeneration from mature embryosis in spring wheat. Int. J. Susutain. Crop Product., 3(2):76-80.
  • Redha A., T. Attia, B. Büter, S. Saisingtong, P. Stamp and J.E. Schmid, 1998. Improved production of doubled haploids by colchcine application to wheat (Triticum aestivum L.) anther culture. Plant Cell Tissue and Organ
  • Culture, 63: 167-172.
  • Redha A., S.M.S. Islam, B. Büter, P. Stamp and J.E. Schmid, 2000. The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer. Plant Cell Tissue and Organ Culture, 63: 167-172.
  • Shahinul Islam S.M. 2010. The effect of colchcine pretreatment on isolated microspore culture of wheat. Australian Journal of Crop Science, 4 (8): 660-665.
  • Szakacs E., G. Kovacs, J. Pauk and B. Barbanas, 1989. Substitution analysis of callus induction and plant regeneration from anter culture in wheat (Triticum aestivum L.) anters. Plant Cell Rep., 7: 218-222.
  • Tuvesson S., A. Ljungberg, N. Johansson, K.E. Karlson, L.W. Suıjs and J.P. Josset, 2000. Large-scale production of wheat and triticale double haploids through the use of a singleanther culture method. Plant Breeding,
  • :455-459.
  • Zamani I., E. Gouli-Vavdinoudi, G. Kovacs, I. Xynıas, D. Roupakias and B. Barbanas, 2003. Plant Breeding, 122: 314-317.
  • Zhou H. and C.F. Konzak, 1989. Improvement of anther culture methods for haploid production in wheat. Crop Science, 29: 817-822.
There are 32 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Ayten Salantur This is me

Selami Yazar

Emin Dönmez

Taner Akar

Publication Date March 1, 2011
Published in Issue Year 2011 Volume: 20 Issue: 1

Cite

APA Salantur, A., Yazar, S., Dönmez, E., Akar, T. (2011). Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 20(1), 15-21.
AMA Salantur A, Yazar S, Dönmez E, Akar T. Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi. March 2011;20(1):15-21.
Chicago Salantur, Ayten, Selami Yazar, Emin Dönmez, and Taner Akar. “Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture”. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 20, no. 1 (March 2011): 15-21.
EndNote Salantur A, Yazar S, Dönmez E, Akar T (March 1, 2011) Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 20 1 15–21.
IEEE A. Salantur, S. Yazar, E. Dönmez, and T. Akar, “Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture”, Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, vol. 20, no. 1, pp. 15–21, 2011.
ISNAD Salantur, Ayten et al. “Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture”. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 20/1 (March 2011), 15-21.
JAMA Salantur A, Yazar S, Dönmez E, Akar T. Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi. 2011;20:15–21.
MLA Salantur, Ayten et al. “Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture”. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, vol. 20, no. 1, 2011, pp. 15-21.
Vancouver Salantur A, Yazar S, Dönmez E, Akar T. Determination of Plant Regeneration Response of Winter Bread Wheat F2 Population under Anther Culture. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi. 2011;20(1):15-21.
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