Sitopatolojik Değerlendirme Süreçleri için Optimum Aralığın Korunmasıyla Yüksek Çözünürlüklü Otomatik Panoramik Görüntüleme

Year 2018, Volume: 11 Issue: 2, 1 - 12, 15.11.2018

Hülya Doğan , Elif Baykal Murat Ekinci , Mustafa Emre Ercin , Şafak Ersöz

Abstract

Mikroskop dar bir görüş alanına sahip olduğu için
sitopatolojik değerlendirme süreçlerinde patologlar numunenin sadece belirli
bir kısmını görebilmektedirler. Numunenin tüm alanını inceleyebilmek için
mikroskop platformunu X-Y-Z yönünde hareket ettirerek numune üzerinde üç
boyutlu tarama yapmaktadırlar. Yapılan çalışmada sitopatolojik değerlendirme
süreçleri otomatikleştirilerek numunenin geniş görüş alanına sahip yüksek
çözünürlüklü panoramik görüntüsünün elde edilmesi amaçlanmaktadır. Panoramik
birleştirme sürecinin otomatikleştirilmesi için yapılan literatür çalışmalarında
ortak alanlı görüntüler oluşturulurken mikroskopta var olan ve mikron cinsinden
ölçülen odaklama derinliği dikkate alınmamaktadır. Bu yüzden ortak alanlı
görüntüler arasında odaklama farklılıkları oluşmakta ve tarama anında bulanık
görüntüler elde edilmektedir. Bu problemi çözmek için çalışmada odaklama
derinliği artırılarak optimum odaklanmış ortak alanlı görüntüler
oluşturulmaktadır. Önerilen yöntemin başarısının ispatı için literatürde
önerilmiş 2 farklı tarama süreci kullanılarak panoramik görüntüler elde
edilmiştir. Oluşturulmuş panoramik görüntüler referans görüntü gerektirmeyen
metrikler kullanılarak karşılaştırılmış ve önerilen yöntemin başarısı hem
sayısal hem de görsel sonuçlarla ispatlanmıştır.

Keywords

Sitopatolojik Değerlendirme, Panoramik Görüntüleme, Odaklama Derinliği, Odaklama Derinliğinin Artırılması

High Resolution Automatic Panoramic Imaging by Maintaining Optimal Range for Cytopathological Analysis

Abstract

Since the microscope has a small field of view,
pathologists can only see a certain part of the specimen during the
cytopathological analysis process. In order to see the whole area of the
sample, they scan the sample in three dimensions by moving the microscope
platform in the X-Y-Z direction. The aim of the study is to obtain a high
resolution panoramic image of the sample by automating the process of
cytopathological analysis. Literature studies for the automation of the
panoramic imaging process do not take into account the depth of focus measured
in microns, which is present in the microscope, while creating images with the
same field of view. This results in differences in focus between the images and
during the scanning process blurred images are obtained. In order to solve this
problem, the depth of focus is extended to produce optimum focused images. To
evaluate the success of the proposed method, panoramic images were obtained
using two different scanning processes suggested in the literature. The
generated panoramic images are compared using the metrics without requiring
reference image and the success of the proposed method is proved by both
quantitative and visual results

Keywords

Cytopathological Analysis, Panoramic Imaging, Depth of Focus, Extended Depth of Field

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