Determination of the soil persistency of native entomopathogenic nematodes applied with a drip irrigation system in a peach orchard

Yıl 2022, , 67 - 74, 05.07.2022

Çiğdem Şahin , Uğur Gözel

https://doi.org/10.31019/tbmd.1090947

Öz

The period of persistence of entomopathogenic nematodes (EPNs) in soil is an important factor in the success of biological control efforts. In this study, the persistency of four native Turkish entomopathogenic nematode species, Steinernema feltiae (Filipjev), S. carpocapsae (Weiser), S. affine (Bovien) and Heterorhabditis bacteriophora (Poinar), was investigated in a peach orchard after their application with a drip irrigation system in Çanakkale Province, Turkiye, in 2018 and 2019. The EPNs were applied at the rate of 50 IJs/cm2. After application, the persistency of the EPNs was determined with soil sampling on a monthly basis. EPN persistence in the soil was 90 days and 150 days in 2018 and 2019, respectively. Additionally, the persistency of the EPNs in soil after their application varied, depending on environmental factors. The longer survival of EPNs in soil after application is important for the increase of the efficiency of EPNs against pests. It was concluded that the local EPN species have potential for use in the biological control of agricultural pests.

Anahtar Kelimeler

Steinernema feltiae, Steinernema carpocapsae, Steinernema affine, Entomopathogenic nematode, Persistency

Destekleyen Kurum

Tübİtak TOVAG

Proje Numarası

116O361

Teşekkür

This study was funded by TUBİTAK TOVAG. Project No: 116O361.

Şeftali bahçesinde damla sulama yöntemi ile toprağa uygulanan yerel entomopatojen nematodların kalıcılığının belirlenmesi

Öz

Entomopatojen nematodların topraktaki hayatta kalma süreleri biyolojik mücadele çalışmalarının başarısı için önemlidir. Bu çalışmada bir şeftali bahçesinde 4 yerel entomopatojen nematod (EPN) türü; Steinernema feltiae Filipjev (Nematoda: Steinernematidae), S. carpocapsae Weiser (Nematoda: Steinernematidae), S. affine Bovien (Nematoda:Steinernematidae) ve Heterorhabditis bacteriophora Poinar (Nematoda: Heterorhabditidae)’nın topraktaki kalıcılığı damla sulama yöntemi ile 2018 ve 2019 yıllarında araştırılmıştır. Entomopatojen nematodlar toprağa 50 IJs/cm2 dozunda uygulanmıştır. Uygulama sonrası ayda bir toprak örneklemesi yapılarak entomopatojen nematodların topraktaki kalıcılığı belirlenmiştir. Araştırma sonucunda 2018 yılında EPN’lerin toprakta kalıcılıklarının 90 gün, 2019 yılında ise 150 gün sürdüğü belirlenmiştir. Elde edilen bulgular neticesinde EPN’lerin toprağa uygulandıktan sonra çevresel faktörlere bağlı olarak kalıcılık sürelerinin değiştiği belirlenmiştir. Uygulama sonrası EPN’lerin toprakta kalma sürelerinin uzatılması topraktaki zararlılara etkinliklerinin artırılması açısından önemlidir.

Anahtar Kelimeler

Steinernema feltiae, Steinernema carpocapsae, Steinernema affine, Entomopatojen nematod, Kalıcılık

Proje Numarası

116O361

Kaynakça

• Bani Mfarrej M.F., 2005. Identification, isolation, identification, and manipulation of almond borer pheromones (Capnodis carbonaria Klug, Buprestidae: Coleoptera). Dissertation, University of Jordan, Ürdün, 66 p.
• Bedding R.A. & R.J. Akhurst, 1975. A simple technique for the detection of insect parasitic Rhabditid nematodes in soil. Nematologica 21: 109-110.
• Deviren S., 2011. Entomopatojen nematodların Hatay ili taş çekirdekli meyve bahçelerinde zararlı Capnodis spp. mücadelesinde kullanılması üzerine araştırmalar. Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Hatay, 42s.
• Garcia-del-Pino F. & A. Morton, 2005. Efficacy of entomopathogenic nematodes against neonate larvae of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae) in laboratory trials. BioControl, 50: 307-316.
• Garrido A. Del Busto T. & J. Malaogn, 1987. Método de recogida de bueuos de Capnodis tenebrionis L. (Col. Buprestidae) y algunos factores abioticos que pueden condicionar la puesta. Boletin de Sanidad Vegetal Plagas, 13: 303-309.
• Gindin G., T. Kuznetsova, A. Protasov, S. Ben Yehuda & Z. Mendel, 2009. Artificial diet for two flat-headed borers, Capnodis spp. (Coleoptera: Buprestidae). European Journal of Entomology, 206: 573-581.
• Glazer I., 1996. Survival mechanisms of entomopathogenic nematodes. Biocontrol Science and Technology, 6(3):373-378.
• Gray P.A. & D.T. Johnson, 1983. Survival of the nematode Neoaplectana carpocapsae in relation to soil temperature, moisture and time. Journal of the Georgia Entomological Society, 18: 454-460.
• Grewal P.S., S. Selvan & R. Gaugler, 1994. Thermal adaptation of entomopathogenic nematodes niche breadth for infection, establishment, and reproduction. Journal of Thermal Biology, 19: 245–253.
• Griffin C., R. Chaerani, D. Fallon, A. Reid & M. Downes, 2000. Occurrence and distribution of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis indica in Indonesia. Journal of Helminthology, 74(2): 143-150.
• Griffin C.T., 1993. Temperature responses of entomopathogenic nematodes: Implications for the success of biological control programs. In: Bedding, R. A., Akhurst, R. J., Kaya, H. K., eds. Nematodes and the biological control of insect pests. East Melbourne CSIRO Publications, 115-126.
• Hazır, S., H.K. Kaya, S.P. Stock & N. Keskin, 2003. Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turkish Journal of Biology, 27: 181-202.
• Karaca, Z. 2012. Malatya ili kayısı bahçelerinde bulunan Capnodis türleri, yoğunlukları ve zarar oranlarının belirlenmesi. Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Hatay, 78s.
• Kaya H.K. & R. Gaugler, 1993. Entomopathogenic nematodes. Annual Review of Entomology, 38(1): 181–206.
• Kaya H.K. & S.P. Stock, 1997. Techniques in insect nematology, in: Manual of Techniques in Insect Pathology, L. A. Lacey, ed Academic Press, London. 281-324.
• Khan Y.S. & N. Javed, 2018. Entomopathogenic nematodes survey, persistence in soil, reproductive potential and their effects on Meloidogyne incognita. Egyptian Journal of Agronematology, 17(2): 109-120.
• Khumalo N.N., T.E. Lephoto & V.M. Gray, 2021. The effect of organic compost and soil texture on the survival and infectivity of entomopathogenic nematode species. Archives of Phytopathology and Plant Protection, 54(17-18): 1443-1455.
• Koppenhöfer, A.M. 2000. Nematodes. In: Lacey, L.A., Kaya, H.K., Eds. Field manual of techniques in vertabrate pathology. Dordrecht, The Netherlands Kluwer, 283-301.
• Koppenhöfer A.M., M.E. Baur, S.P. Stock, H.Y. Choo, B. Chinnasri & H.K. Kaya, 1997. Survival of entomopathogenic nematodes within host cadavers in dry soil. Applied Soil Ecology, 6(3): 231-240.
• Martinez de Altube M.M., O. Strauch, G.F. De Castro & A.M. Pena, 2008. Control of the flat-headed root borer Capnodis tenebrionis (Linne) (Coleoptera: Buprestidae) with the entomopathogenic nematode Steinernema carpocapsae (Weiser) (Nematoda: Steinernematidae) in a chitosan formulation in apricot orchards. BioControl, 53(3): 531-539.
• Morton A. & F. Garcia Del Pino, 2008. Field efficacy of the entomopathogenic nematode Steinernema feltiae against the Mediterranean flat-headed rootborer Capnodis tenebrionis. Journal of Applied Entomology, 132(8): 632-637.
• Noitubtim P., B. Caoili & A. Noosidum, 2022. Productivity of five entomopathogenic nematodes in Galleria mellonella L. and their persistence in soil under laboratory conditions. International Journal of Agricultural Technology, 18(2): 667-678.
• Perez E.E., E.E. Lewis & D.I. Shapiro-Ilan, 2003. Impact of host cadaver on survival and infectivity of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) under desiccating conditions. Journal of Invertebrate Pathology, 82: 111-118.
• Şahin Ç. & U. Gözel, 2017. Preliminary studies on distribution and biology of Capnodis tenebrionis L. (Coleoptera: Buprestidae) in Çanakkale Province. ISEEP, 4-7 Ekim, 2017, Çanakkale, 262p.
• Şahin Ç. & U. Gözel, 2019. Efficacy of Entomopathogenic Nematodes Against Neonate Larvae of Capnodis tenebrionis (L., 1758) (Coleoptera: Buprestidae). Türkiye Entomoloji Dergisi, 43(3): 279-285.
• Şahin Ç. & U. Gözel, 2021. Efficacy and persistence of native entomopathogenic nematodes against Capnodis tenebrionis in peach (Prunus persica) orchard in Turkey. Phytoparasitica, 49 (3):1-9.
• Shapiro-Ilan D.I., E.E. Lewis, W.L. Tedders & Y. Son, 2003. Superior efficacy observed in entomopathogenic nematodes applied in infected-host cadavers compared with application in aqueous suspension. Journal of Invertebrate Pathology, 83: 270–272.
• Shields E.J., A.M. Testa & W.J. O’Neil, 2018. Long-term persistence of native New York entomopathogenic nematode isolates across crop rotation. Biological and Microbial Control, 111(6): 2592–2598.
• Stock S.P., B.M. Pryor & H.K. Kaya, 1999. Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California, USA. Biodiversity Conservation, 8: 535–549.
• Susurluk A. & R.U. Ehlers, 2008. Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl, 53 (4): 627-641.
• White G.F., 1929. A method for obtaining infective nematode larvae from cultures. Science, 66:302–303. Womersley C.Z., 1990. Dehydration survival and anhydrobiotic potential. In: Gaugler R, Kaya HK, editors. Entomopathogenic nematodes in biological control. Boca Raton (FL): CRC Press. p. 117–137.
• Zobar D. & M. Kıvan, 2019. Tekirdağ ilinde farklı anaçlı kiraz bahçelerinde Capnodis tenebrionis (L.) (Coleoptera: Buprestidae)’in mevsimsel gelişimi. Journal of Tekirdağ Agriculture Faculty, 16(3): 339-347.