Toxicological Analysis of a New Fibrin-Derived Dermal Scaffold (Dermoturk); Acellular and Combined with Stem Cells Forms

Year 2024, , 130 - 139, 18.03.2024

Emrah Şenel , Sabri Demir , Aysun Gökce , Murat Alper , Mesut Akyol , Fatma Eyüboğlu , Ercüment Ovalı

https://doi.org/10.12956/tchd.1405903

Abstract

Objective: We aimed to reveal the toxicological analysis of the newly developed fibrin-derived scaffold forms (DermoTurk) before human studies.
Material and Methods: 42 male Albino Wistar rats were used. Two of them were used to produce mesenchymal and epidermal stem cells. Forty rats were divided into five groups, each consisting of 8 rats; the acellular scaffold applied group as Group-1, the mesenchymal stem cells added scaffold used group as Group-2, the MSCs and epidermal stem cells-added scaffold applied group as Group-3, MSCs- and epidermal stem cells-added scaffold applied outbred group as Group-4 and control as Group-5. The changing of laboratory tests in the groups was evaluated five days before application and on the 7th and 40th days. After the autopsy performed on the 40th day of the study, rats’ organs and scaffold implanted skin area were evaluated histologically. All the results of the groups were compared. SPSS 22.0 was used for analyses. P <0.05 was accepted as statistically significant.
Results: There were no differences between the groups in terms of laboratory results. Histologically, a mild-grade foreign body reaction against the DermoTurk was found in all groups; this reaction was less in groups 3 and 4 with the richest stem cells.
Conclusion: This study revealed that DermoTurk is safe in rats. It could be an important alternative to skin substitutes, with stem cells or alone. Human studies for clinical efficacy should be carried out.

Keywords

Mesenchymal stem cell, epidermal stem cell, tissuue engineering, burn, fibrin-derived dermal scaffold

Fibrinden Türetilmiş Yeni Bir Dermal matriksin (DermoTurk) Aselüler ve Kök Hücreler ile Kombine Formlarının Toksikolojik Analizi

Abstract

Amaç: Bu çalışmamızda yeni geliştirdiğimiz fibrinden türetilmiş dermal matriksin (DermoTurk) değişik formlarının insan çalışmalarından önce yaptığımız toksikoloji testlerinin sonuçlarını paylaşmayı amaçladık.
Gereç ve Yöntemler: Çalışmada 42 adet erkek Albino-Wistar rat kullanıldı. Bunlardan ikisi mezenkimal ve epidermal kök hücre üretmek için kullanıldı. Kırk tane rat ise her biri 8 denekten oluşan beş gruba ayrıldı; aselüler matriks uygulanan grup (Grup-1), fibrin matriks ile mezenkimal kök hücre (MKH) uygulanan grup (Grup-2), fibrin matriks ile MKH'ler ve epidermal kök hücrelerin (EKH) uygulandığı inbread grup (Grup-3), fibrin matrilks ile MKH'ler ve EKH'lerin uygulandığı outbread grup (Grup-4), kontrol ise Grup-5 olarak belirlendi. Gruplardaki laboratuvar testlerinin sonuçları uygulamadan beş gün önce ve uygulamanın 7. ve 40. günlerinde değerlendirildi. Çalışmanın 40. gününde yapılan otopsinin ardından deneklerin organları ve fibrin matriks implante edilen cilt alanı histolojik olarak değerlendirildi. Grupların tüm sonuçları karşılaştırıldı. İstatistiksel analiz SPSS 22.0 ile yapıldı. P<0,05 istatistiksel olarak anlamlı kabul edildi.
Bulgular: Laboratuvar sonuçları açısından gruplar arasında fark yoktu. Histolojik olarak tüm gruplarda DermoTurk'e karşı hafif derecede yabancı cisim reaksiyonu saptandı; bu reaksiyon kök hücrelerin bulunduğu 3. ve 4. gruplarda daha azdı.
Sonuç: Çalışmamız fibrinden türetilen DermoTurk'ün ratlarda güvenli olduğunu göstermiştir. Bu matriks kök hücrelerle veya tek başına diğer deri benzerlerine önemli bir alternatif olabilir. Klinik etkinliliğin gösterilmesi için insan çalışmaları yapılmalıdır.

Keywords

Mezenkimal kök hücreler, epidermal kök hücreler, doku mühendisliği, yanık, fibrinden türetilmiş dermal matriks

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