TIBBİ MİKROBİYOLOJİ LABORATUVARI AÇISINDAN SARS-CoV-2

Sibel Aydoğan; Bedia Dinç

doi:10.12956/tchd.741463

Review

Year 2020, COVID-19, 18 - 25, 24.07.2020

Sibel Aydoğan , Bedia Dinç

https://doi.org/10.12956/tchd.741463

Cited By: 1

Abstract

Keywords

Coronavirus, SARS-Cov-2, Diagnosis

References

1. Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2.Nature Microbiology 2020;5(4):536–544.
2. https://www.who.int/emergencies/diseases/novel-coronavirus-2019
3. Cui J, Li F, Daszak P. Origin and evolution of pathogenic coronaviruses. Nat Rev Genet 2018; 17: 181–192.
4. Su S, Wong G, Shi W, Liu J, Lai A C K, Zhou J et al. Epidemiology, genetic recombination, and Pathogenesis of coronaviruses.Trends Microbiol 2016; 24(6):490–502. doi: 10.1016/j.tim.2016.03.003.
5. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 2003; 348(20): 1953–1966.
6. Peiris JS, Lai ST, Poon LL, Guan Y, Yam LY, Lim W et al. Coronavirus as a possible cause of severe acute respiratory syndrome.Lancet 2003; 361(9366):1319–1325. doi: 10.1016/S0140-6736(03)13077-2.
7. Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA.Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 2012; 367(19): 1814–1820 doi: 10.1056/NEJMoa1211721. Epub 2012 Oct 17. Erratum in: N Engl J Med. 2013 Jul 25;369(4):394.
8. Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, Al-Rabiah FA, Al-Hajjar S, Al-Barrak A et al. Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. Lancet Infect Dis 2013;13(9):752–761. doi: 10.1016/S1473-3099(13)70204-4.
9. Park SE. Epidemiology, virology, and clinical features of severe acute respiratory syndrome - coronavirus-2 (SARS-CoV-2; Coronavirus Disease-19). Clin Exp Pediatr 2020 Apr;63(4):119-124.doi: 10.3345/cep.2020.00493.
10. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W et al. A Pneumonia Outbreak Associated with a New Coronavirus of Probable Bat Origin. Nature 2020, 579, 270.
11. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J et al. A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med 2020; 382 (8): 727−733.
12. Wu A, Peng Y, Huang B, Ding X, Wang X, Niu P et al. Commentary Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China. Cell Host Microbe 2020 Mar 11;27(3):325-328. doi: 10.1016/j.chom.2020.02.001. Epub 2020 Feb 7.
13. Ren LL, Wang YM, Wu ZQ, Xiang ZC, Guo L, Xu T, et al. Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study. Chin Med J (Engl) 2020 May 5;133(9):1015-1024. doi: 10.1097/CM9.0000000000000722.
14. Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating personto-person transmission: a study of a family cluster. Lancet 2020;395(10223):514-523.doi:10.1016/S0140-6736(20)30154-9. Epub 2020 Jan 24.
15. Song Z, Xu Y, Bao L, Zhang L, Yu P, Qu Y, et al. From SARS to MERS, thrusting coronaviruses into the spotlight. Viruses 2019;11(1):59. doi: 10.3390/v11010059.
16. Cui J, Li F, Shi ZL. Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol 2019;17(3):181–192. doi: 10.1038/s41579-018-0118-9.
17. Chen Y, Liu Q, Guo D. Emerging coronaviruses: Genome structure, replication, and pathogenesis. Journal of Medical Virology 2020; 92 (4): 418-423. doi: 10.1002/jmv.25681.
18. Hasoksuz M, Sreevatson S, Cho KO, Hoet AE, Saif LJ. Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates. Virus Research 2002; 84 (1-2): 101-109. doi: 10.1016/S0168- 1702(02)00004-7.
19. Hasoksuz M, Lathrop S, Al-dubaib MA, Lewis P, Saif LJ. Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected using monoclonal antibodies. Archives of Virology 1999; 144 (12): 2441-2447.
20. Bosch BJ, Van Der Zee R, De Haan CAM, Rottier PJM. The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex. Journal of Virology 2003; 77 (16): 8801-8811. doi: 10.1128/JVI.77.16.8801-8811.2003.
21. Hamming I, Timens W, Bulthuis MLC, Lely AT, Navis GJ, van Goor H. Tissue Distribution of ACE2 Protein, the Functional Receptor for SARS Coronavirus. A First Step in Understanding SARS Pathogenesis. J. Pathol 2004; 203 (2): 631− 637.
22. Neuman B, Kiss G, Kunding A, Bhella D, Baksh MF, Connelly S et al. A structural analysis of M protein in coronavirus assembly and morphology. J Struct Boil 2010; 174: 11–22.
23. DeDiego ML, Álvarez E, Almazán F, Rejas MT, Lamirande E, Roberts A et al. A severe acute respiratory syndrome coronavirus that lacks the E Gene is attenuated in vitro and in vivo. J Virol 2006; 81: 1701–1713.
24. Fehr AR, Perlman S. Coronaviruses: An overview of their replication and pathogenesis. In Therapeutic Antibodies; Springer: Berlin/Heidelberg, Germany, 2015; Volume 1282, pp. 1–23.
25. Cui L, Wang H, Ji Y, Yang J, Xu S, Huang X et al. The nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells. J Virol 2015; 89: 9029–9043.
26. Liu YC, Kuo RL, Shih SR. COVID-19: the First Documented Coronavirus Pandemic in History. Biomed J 2020 May 5:S2319-4170(20)30044-5. doi: 10.1016/j.bj.2020.04.007.
27. World Health Organization.Laboratory testing for coronavirus disease (COVID-19) in suspected human cases.19 March 2020.
28. Loeffelholz MJ, Tang YW. Laboratory diagnosis of emerging human coronavirus infections-the state of the art. Emerg Microbes Infect 2020; 9(1):747-56.
29. Kaye M, Druce J, Tran T, Kostecki R, Chibo D, Morris J, et al. SARS associated coronavirus replication in cell lines. Emerg Infect Dis 2006;12(1):128-133.
30. www.cdc.gov May 5, 2020. SARS CoV-2 viral culture at CDC.
31. Patel R, Babady E, Theel ES, Storch GA, Pinsky BA, George K, et al. Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of diagnostic testing for SARS-CoV-2/COVID-19. American Society for Microbiology 2020;11(2):1-4.
32. Khailany RA, Safdar M, Ozaslan M. Genomic characterization of a novel SARS CoV-2. Gene Reports 2020 Apr 16;19:100682. doi: 10.1016/j.genrep.2020.100682.
33. Udugama B, Kadhiresan P, Kozlowski HN, Malekjahani A, Osborne M, Li VYC, et al. Diagnosing COVID-19: The disease and tools for detection. ACS Nano 2020; 14: 3822-35.
34. Sheridan C. Coronavirus and the race to distribute reliable diagnostics. Nat Biotechnol 2020; DOI:10.1038/d41587-020-00002-2.
35. Miller S, Chiu C, Rodino KG, Miller MB. Point-counterpoint should we be performing metagenomic next-generation sequencing for infectious disease diagnosis in the clinical laboratory? J Clin Microbiol 2020; DOI:10.1128/JCM.01739-19.
36. Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR. Pitfalls and potential. BioTechniques 1999; 26(1):124-125.
37. Corman V, Bleicker T, Brünink S, Zambon M. Diagnostics detection of Wuhan Coronavirus 2019 by real-time RT-PCR. WHO: Geneva 2020.
38. CDC-2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel, Division of Viral Disease, U.S. Centers for Disease Control and Prevention: Atlanta GA, 2020.
39. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: Interim guidance, World Health Organization 2 March 2020.
40. Cheng MP, Papenburg J, Desjardins M, Kanjilal S, Quach C, Libman M, et al. Diagnostic testing for severe acute respiratory syndrome-related Coronavirus-2. Ann Intern Med Doi.10.7326/M20-1301.
41. Lan L, Xu D, Ye G, Xia C, Wang S, Li Y, et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA, 2020 Feb, 27. doi:10.1001/jama2020.2783.
42. Zou L, Ruan F, Huang M, Liang L, Huang H, Hang Z, et al. SARS CoV-2 viral load in upper respiratory specimens of infected patients. N Eng J Med 2020; 382: (12):1177-79
43. Pan Y, Zhang D, Yang P, Poon LLM, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis 2020; 20(4):411-12. 44. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveill 2020; 25(3):2000045.
45. Chu DK, Pan Y, Cheng SM, Hui KP, Krishnan P, Liu Y, et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. Clin Chem 2020; 66:549-55.
46. XpertXpress SARS-CoV-2. https://www.cepheid.com/coronavirus.
47. Abbott launches molecular point-of-care test to detect novel coronavirus in as little as five minutes. https://abbott.mediaroom.com/2020-03-27.
48. Xiang J, Yan M, Hongze L, Ting L, Chenyao L, Shueng H, et al. Evaluation of enzyme-linked immunoassay and colloidal gold- immunochromatographic assay kit for detection of novel coronavirus (SARS-Cov-2) causing an outbreak of pneumonia (COVID-19). medRxiv, doi: 10.1101/2020.02.22.20026617.
49. Bruning AHL, Aatola H, Toivola H, Ikonen N, Savolainen-Kopra C, Blomquist S, et al. Rapid detection and monitoring of human coronavirus infections. New Microbes New Infect 2018; 24:52-55.
50. Sheridan C. Fast portable tests come online to curb coronavirus pandemic. Nat Biotechnol 2020; doi:10.1038/d41587-020-00010-2.
51. Chan CM, Tse H, Wong SS, Woo PCY, Lau SKP, Chen L, et al. Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus. J Clin Virol 2009; 45(1):54-60.
52. Shao X, Guo X, Esper F, Weibel C, Kahn JS. Seroepidemiology of group I human coronaviruses in children. J Clin Virol 2007; 40(3):207-13.
53. Infantino M, Damiani A, Gobbi FL, Grossi V, Lari B, Macchia D, et al. Serological assays for SARS-CoV2 ınfectious disease:Benefits, limitations and perspectives.IMAJ 2020; 22:203-10.
54. Che XY, Qiu LW, Liao ZY, Wang YD, Wen K, Pan YX, et al. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J Infect Dis 2005;191:2033-7.
55. Carattoli A, Di Bonito P, Grasso F, Giorgi C, Blasi F, Niedrig M, et al. Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS. J Med Virol 2005; 76:137-42.
56. Walls AC, Park YJ, Tortorici MA, Wall A, MacGuire AT, Veesler D. Structure, function, and antigenicity of the SARS CoV-2 spike glycoprotein. Cell 2020 Apr 16;181(2):281-292.e6. doi: 10.1016/j.cell.2020.02.058.Epub 2020 Mar 9.
57. Okba NMA, Müller MA, Li W, Wang C, GeurtsvanKessel CH, Corman VM, Lamers MM, et al. SARS-CoV-2 specific antibody responses in COVID-19 patients. MedRwix preprint on March 20, 2020.
58. Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin Infect Dis 2020 Mar 28. pii: ciaa344. doi: 10.1093/cid/ciaa344.
59. Guo L, Ren L, Yang S, Xiao M, Chang D , Yang F, et al. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19). Clin Infect Dis 2020 Mar 21. pii: ciaa310. doi: 10.1093/cid/ciaa310.
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TIBBİ MİKROBİYOLOJİ LABORATUVARI AÇISINDAN SARS-CoV-2

Year 2020, COVID-19, 18 - 25, 24.07.2020

Sibel Aydoğan , Bedia Dinç

https://doi.org/10.12956/tchd.741463

Cited By: 1

Abstract

Coronaviridae ailesinde yer alan Koronaviruslar (CoV), vahşi ve evcil hayvanlarda farklı şiddette gastrointestinal, respiratuvar ve sistemik hastalıklara neden olurken, insanlarda bağışıklık durumuna göre soğuk algınlığından pnömoniye kadar farklı klinik tablolara yol açabilir. Günümüze dek insanlarda enfeksiyon etkeni olarak yedi koronavirus türü tanımlanmıştır; bunlardan HCoV 229E, HCoV NL63, HCoV HKU1 ve HCoV OC43, tipik olarak immünokompetan bireylerde soğuk algınlığı semptomlarına neden olurken, SARS-CoV (Ciddi Akut Solunum Sendromu Koronavirus), MERS-CoV (Orta Doğu Solunum Sendromu Koronavirus) zoonotiktir ve ciddi solunum yolu hastalıklarına ve ölümlere neden olur. Çin’in Hubei Eyaleti, Wuhan kentinde Aralık 2019’da başlayan ve kısa sürede pandemi olarak tanımlanan COVID -19’un etkeni SARS-CoV-2 insanlarda enfeksiyon etkeni olarak tanımlanan yedinci koronavirustur. Dünya Sağlık Örgütü(DSÖ)’nün SARS-CoV-2 kaynaklı COVID-19’u pandemi olarak tanımlaması ve dünya üzerinde her geçen gün vaka ve ölüm sayılarının artmasından dolayı, aşı çalışmaları için virusun yapısı ve salgını kontrol etmede en önemli basamak olan viral tanı yöntemlerinin kullanılması daha da önem kazanmıştır.

Keywords

Koronavirus, SARS-CoV-2, Teşhis

References

1. Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2.Nature Microbiology 2020;5(4):536–544.
2. https://www.who.int/emergencies/diseases/novel-coronavirus-2019
3. Cui J, Li F, Daszak P. Origin and evolution of pathogenic coronaviruses. Nat Rev Genet 2018; 17: 181–192.
4. Su S, Wong G, Shi W, Liu J, Lai A C K, Zhou J et al. Epidemiology, genetic recombination, and Pathogenesis of coronaviruses.Trends Microbiol 2016; 24(6):490–502. doi: 10.1016/j.tim.2016.03.003.
5. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 2003; 348(20): 1953–1966.
6. Peiris JS, Lai ST, Poon LL, Guan Y, Yam LY, Lim W et al. Coronavirus as a possible cause of severe acute respiratory syndrome.Lancet 2003; 361(9366):1319–1325. doi: 10.1016/S0140-6736(03)13077-2.
7. Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA.Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 2012; 367(19): 1814–1820 doi: 10.1056/NEJMoa1211721. Epub 2012 Oct 17. Erratum in: N Engl J Med. 2013 Jul 25;369(4):394.
8. Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, Al-Rabiah FA, Al-Hajjar S, Al-Barrak A et al. Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. Lancet Infect Dis 2013;13(9):752–761. doi: 10.1016/S1473-3099(13)70204-4.
9. Park SE. Epidemiology, virology, and clinical features of severe acute respiratory syndrome - coronavirus-2 (SARS-CoV-2; Coronavirus Disease-19). Clin Exp Pediatr 2020 Apr;63(4):119-124.doi: 10.3345/cep.2020.00493.
10. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W et al. A Pneumonia Outbreak Associated with a New Coronavirus of Probable Bat Origin. Nature 2020, 579, 270.
11. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J et al. A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med 2020; 382 (8): 727−733.
12. Wu A, Peng Y, Huang B, Ding X, Wang X, Niu P et al. Commentary Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China. Cell Host Microbe 2020 Mar 11;27(3):325-328. doi: 10.1016/j.chom.2020.02.001. Epub 2020 Feb 7.
13. Ren LL, Wang YM, Wu ZQ, Xiang ZC, Guo L, Xu T, et al. Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study. Chin Med J (Engl) 2020 May 5;133(9):1015-1024. doi: 10.1097/CM9.0000000000000722.
14. Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating personto-person transmission: a study of a family cluster. Lancet 2020;395(10223):514-523.doi:10.1016/S0140-6736(20)30154-9. Epub 2020 Jan 24.
15. Song Z, Xu Y, Bao L, Zhang L, Yu P, Qu Y, et al. From SARS to MERS, thrusting coronaviruses into the spotlight. Viruses 2019;11(1):59. doi: 10.3390/v11010059.
16. Cui J, Li F, Shi ZL. Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol 2019;17(3):181–192. doi: 10.1038/s41579-018-0118-9.
17. Chen Y, Liu Q, Guo D. Emerging coronaviruses: Genome structure, replication, and pathogenesis. Journal of Medical Virology 2020; 92 (4): 418-423. doi: 10.1002/jmv.25681.
18. Hasoksuz M, Sreevatson S, Cho KO, Hoet AE, Saif LJ. Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates. Virus Research 2002; 84 (1-2): 101-109. doi: 10.1016/S0168- 1702(02)00004-7.
19. Hasoksuz M, Lathrop S, Al-dubaib MA, Lewis P, Saif LJ. Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected using monoclonal antibodies. Archives of Virology 1999; 144 (12): 2441-2447.
20. Bosch BJ, Van Der Zee R, De Haan CAM, Rottier PJM. The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex. Journal of Virology 2003; 77 (16): 8801-8811. doi: 10.1128/JVI.77.16.8801-8811.2003.
21. Hamming I, Timens W, Bulthuis MLC, Lely AT, Navis GJ, van Goor H. Tissue Distribution of ACE2 Protein, the Functional Receptor for SARS Coronavirus. A First Step in Understanding SARS Pathogenesis. J. Pathol 2004; 203 (2): 631− 637.
22. Neuman B, Kiss G, Kunding A, Bhella D, Baksh MF, Connelly S et al. A structural analysis of M protein in coronavirus assembly and morphology. J Struct Boil 2010; 174: 11–22.
23. DeDiego ML, Álvarez E, Almazán F, Rejas MT, Lamirande E, Roberts A et al. A severe acute respiratory syndrome coronavirus that lacks the E Gene is attenuated in vitro and in vivo. J Virol 2006; 81: 1701–1713.
24. Fehr AR, Perlman S. Coronaviruses: An overview of their replication and pathogenesis. In Therapeutic Antibodies; Springer: Berlin/Heidelberg, Germany, 2015; Volume 1282, pp. 1–23.
25. Cui L, Wang H, Ji Y, Yang J, Xu S, Huang X et al. The nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells. J Virol 2015; 89: 9029–9043.
26. Liu YC, Kuo RL, Shih SR. COVID-19: the First Documented Coronavirus Pandemic in History. Biomed J 2020 May 5:S2319-4170(20)30044-5. doi: 10.1016/j.bj.2020.04.007.
27. World Health Organization.Laboratory testing for coronavirus disease (COVID-19) in suspected human cases.19 March 2020.
28. Loeffelholz MJ, Tang YW. Laboratory diagnosis of emerging human coronavirus infections-the state of the art. Emerg Microbes Infect 2020; 9(1):747-56.
29. Kaye M, Druce J, Tran T, Kostecki R, Chibo D, Morris J, et al. SARS associated coronavirus replication in cell lines. Emerg Infect Dis 2006;12(1):128-133.
30. www.cdc.gov May 5, 2020. SARS CoV-2 viral culture at CDC.
31. Patel R, Babady E, Theel ES, Storch GA, Pinsky BA, George K, et al. Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of diagnostic testing for SARS-CoV-2/COVID-19. American Society for Microbiology 2020;11(2):1-4.
32. Khailany RA, Safdar M, Ozaslan M. Genomic characterization of a novel SARS CoV-2. Gene Reports 2020 Apr 16;19:100682. doi: 10.1016/j.genrep.2020.100682.
33. Udugama B, Kadhiresan P, Kozlowski HN, Malekjahani A, Osborne M, Li VYC, et al. Diagnosing COVID-19: The disease and tools for detection. ACS Nano 2020; 14: 3822-35.
34. Sheridan C. Coronavirus and the race to distribute reliable diagnostics. Nat Biotechnol 2020; DOI:10.1038/d41587-020-00002-2.
35. Miller S, Chiu C, Rodino KG, Miller MB. Point-counterpoint should we be performing metagenomic next-generation sequencing for infectious disease diagnosis in the clinical laboratory? J Clin Microbiol 2020; DOI:10.1128/JCM.01739-19.
36. Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR. Pitfalls and potential. BioTechniques 1999; 26(1):124-125.
37. Corman V, Bleicker T, Brünink S, Zambon M. Diagnostics detection of Wuhan Coronavirus 2019 by real-time RT-PCR. WHO: Geneva 2020.
38. CDC-2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel, Division of Viral Disease, U.S. Centers for Disease Control and Prevention: Atlanta GA, 2020.
39. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: Interim guidance, World Health Organization 2 March 2020.
40. Cheng MP, Papenburg J, Desjardins M, Kanjilal S, Quach C, Libman M, et al. Diagnostic testing for severe acute respiratory syndrome-related Coronavirus-2. Ann Intern Med Doi.10.7326/M20-1301.
41. Lan L, Xu D, Ye G, Xia C, Wang S, Li Y, et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA, 2020 Feb, 27. doi:10.1001/jama2020.2783.
42. Zou L, Ruan F, Huang M, Liang L, Huang H, Hang Z, et al. SARS CoV-2 viral load in upper respiratory specimens of infected patients. N Eng J Med 2020; 382: (12):1177-79
43. Pan Y, Zhang D, Yang P, Poon LLM, Wang Q. Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis 2020; 20(4):411-12. 44. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveill 2020; 25(3):2000045.
45. Chu DK, Pan Y, Cheng SM, Hui KP, Krishnan P, Liu Y, et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. Clin Chem 2020; 66:549-55.
46. XpertXpress SARS-CoV-2. https://www.cepheid.com/coronavirus.
47. Abbott launches molecular point-of-care test to detect novel coronavirus in as little as five minutes. https://abbott.mediaroom.com/2020-03-27.
48. Xiang J, Yan M, Hongze L, Ting L, Chenyao L, Shueng H, et al. Evaluation of enzyme-linked immunoassay and colloidal gold- immunochromatographic assay kit for detection of novel coronavirus (SARS-Cov-2) causing an outbreak of pneumonia (COVID-19). medRxiv, doi: 10.1101/2020.02.22.20026617.
49. Bruning AHL, Aatola H, Toivola H, Ikonen N, Savolainen-Kopra C, Blomquist S, et al. Rapid detection and monitoring of human coronavirus infections. New Microbes New Infect 2018; 24:52-55.
50. Sheridan C. Fast portable tests come online to curb coronavirus pandemic. Nat Biotechnol 2020; doi:10.1038/d41587-020-00010-2.
51. Chan CM, Tse H, Wong SS, Woo PCY, Lau SKP, Chen L, et al. Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus. J Clin Virol 2009; 45(1):54-60.
52. Shao X, Guo X, Esper F, Weibel C, Kahn JS. Seroepidemiology of group I human coronaviruses in children. J Clin Virol 2007; 40(3):207-13.
53. Infantino M, Damiani A, Gobbi FL, Grossi V, Lari B, Macchia D, et al. Serological assays for SARS-CoV2 ınfectious disease:Benefits, limitations and perspectives.IMAJ 2020; 22:203-10.
54. Che XY, Qiu LW, Liao ZY, Wang YD, Wen K, Pan YX, et al. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J Infect Dis 2005;191:2033-7.
55. Carattoli A, Di Bonito P, Grasso F, Giorgi C, Blasi F, Niedrig M, et al. Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS. J Med Virol 2005; 76:137-42.
56. Walls AC, Park YJ, Tortorici MA, Wall A, MacGuire AT, Veesler D. Structure, function, and antigenicity of the SARS CoV-2 spike glycoprotein. Cell 2020 Apr 16;181(2):281-292.e6. doi: 10.1016/j.cell.2020.02.058.Epub 2020 Mar 9.
57. Okba NMA, Müller MA, Li W, Wang C, GeurtsvanKessel CH, Corman VM, Lamers MM, et al. SARS-CoV-2 specific antibody responses in COVID-19 patients. MedRwix preprint on March 20, 2020.
58. Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin Infect Dis 2020 Mar 28. pii: ciaa344. doi: 10.1093/cid/ciaa344.
59. Guo L, Ren L, Yang S, Xiao M, Chang D , Yang F, et al. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19). Clin Infect Dis 2020 Mar 21. pii: ciaa310. doi: 10.1093/cid/ciaa310.
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There are 61 citations in total.

Details

Primary Language	Turkish
Subjects	Internal Diseases
Journal Section	REVIEW
Authors	Sibel Aydoğan 0000-0001-8820-032X Bedia Dinç This is me 0000-0001-8318-2556
Publication Date	July 24, 2020
Submission Date	May 22, 2020
Published in Issue	Year 2020 COVID-19

Cite

Vancouver	Aydoğan S, Dinç B. TIBBİ MİKROBİYOLOJİ LABORATUVARI AÇISINDAN SARS-CoV-2. Türkiye Çocuk Hast Derg. 2020;14(COVID-19):18-25.

Cited By

Covid-19 Pandemisinde Mikrobiyoloji Laboratuvar Tanı Metodları

Sakarya Medical Journal

https://doi.org/10.31832/smj.796411

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The publication language of Turkish Journal of Pediatric Disease is English.

Manuscripts submitted to the Turkish Journal of Pediatric Disease will go through a double-blind peer-review process. Each submission will be reviewed by at least two external, independent peer reviewers who are experts in the field, in order to ensure an unbiased evaluation process. The editorial board will invite an external and independent editor to manage the evaluation processes of manuscripts submitted by editors or by the editorial board members of the journal. The Editor in Chief is the final authority in the decision-making process for all submissions. Articles accepted for publication in the Turkish Journal of Pediatrics are put in the order of publication, with at least 10 original articles in each issue, taking into account the acceptance dates. If the articles sent to the reviewers for evaluation are assessed as a senior for publication by the reviewers, the section editor and the editor considering all aspects (originality, high scientific quality and citation potential), it receives publication priority in addition to the articles assigned for the next issue.

The aim of the Turkish Journal of Pediatrics is to publish high-quality original research articles that will contribute to the international literature in the field of general pediatric health and diseases and its sub-branches. It also publishes editorial opinions, letters to the editor, reviews, case reports, book reviews, comments on previously published articles, meeting and conference proceedings, announcements, and biography. In addition to the field of child health and diseases, the journal also includes articles prepared in fields such as surgery, dentistry, public health, nutrition and dietetics, social services, human genetics, basic sciences, psychology, psychiatry, educational sciences, sociology and nursing, provided that they are related to this field. can be published.