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GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ

Year 2022, , 111 - 116, 28.12.2022
https://doi.org/10.46810/tdfd.1092040

Abstract

Bu çalışmada, gebe ve gebe olmayan ineklerde asprosin düzeylerinin tespit edilmesi, bazı biyokimyasal parametreler ile asprosin düzeyleri arasındaki ilişkinin belirlenmesi amaçlanmıştır. Bu amaçla 60 adet Simental ırkı inek, gebe olan (n=30) ve postpartum 15. günde (n=30) gebe olmayan inekler olmak üzere iki gruba ayrıldı ve ineklerden bir kez kan alınarak asprosin düzeyleri ve biyokimyasal parametreler ölçüldü. Ölçülen biyokimyasal parametreler Alkalen fosfataz (ALP), Amilaz, Kreatin kinaz miyokardial band (CK-MB), Kreatin kinaz N-asetil sistein (CK-NAC), Gama glutamil transferaz (GGT), Aspartat Aminotransferaz (AST), Alanin aminotransferaz (ALT), Direkt bilirubin, Total bilirubin , Kalsiyum (Ca), Kolin (CHO), Kreatin, Glikoz, Total protein, Üre, Albümin, Demir, Fosfor, Lacktat dehidrogenaz (LDH-P), High-density lipoprotein kolesterol (HDL-C), Low-density lipoprotein (LDL), Trigliserit, Magnezyum (Mg) ve Unsaturated iron binding capacity (UIBC)’dir. Asprosin düzeyleri ticari ELİSA kitleri kullanılarak, biyokimyasal parametreler ise otoanalizörde ölçüldü. Asprosin düzeyleri ve biyokimyasal parametreler hem grup için hem de gruplar arası karşılaştırılarak sonuçlar değerlendirildi. Elde edilen veriler ışığında gebe olmayan ineklerin direkt biluribin ve total biluribin miktarı gebe ineklerden yüksek, CHO, HDL-C, LDL ve asprosin miktarları ise düşük bulundu. Gebe ineklerin asprosin ile biyokimyasal parametreleri karşılaştırıldığında ise asprosin ile albümin, direkt biluribin, total biluribin ve kreatin miktarları arasında pozitif yönde korelasyon bulundu. Gebe olmayan ineklerde ise asprosin ile amilaz ve fosfor arasında negatif korelasyon, asprosin ile kreatin ve glikoz arasında pozitif yönde korelasyon gözlendi. Sonuç olarak, gebe ineklerde asprosin düzeylerinin daha yüksek olduğu ve bu hormonla biyokimyasal parametreler arasında negatif veya pozitif yönde korelasyonlar olabileceği kanaatine varıldı.

References

  • 1. Rossato W, Gonzalez F, Dias M, Ricco D, Valle S F, Rosa V, et al. Number of lactations affects metabolic profile of dairy cows. Arch Vet Sci. 2001; 6(2): 83-8.
  • 2. Payne J, Dew SM, Manston R, Faulks M. The use of a metabolic profile test in dairy herds. Vet Rec. 1970; 87: 150-8.
  • 3. Romere C, Duerrschmid C, Bournat J, Constable P, Jain M, Xia F, et al. Asprosin, a fasting-induced glucogenic protein hormone. Cell 2016; 165(3): 566-79.
  • 4. Alan M, Gurlek B, Yilmaz A, Aksit M, Aslanipour B, Gulhan I, et al. Asprosin: a novel peptide hormone related to insulin resistance in women with polycystic ovary syndrome. Gynecol Endocrinol. 2019; 35(3); 220-3.
  • 5. Duerrschmid C, He Y, Wang C, Li C, Bournat J C, Romero C, et al. Asprosin is a centrally acting orexigenic hormone. Nat Med. 2017; 23(12): 1444-53.
  • 6. Yuan M, Li W, Zhu Y, Yu B, Wu J. Asprosin: a novel player in metabolic diseases. Front Endocrinol. 2020; 11: 1-7.
  • 7. Ugur K, Aydin S. Saliva and blood asprosin hormone concentration associated with obesity. Int J Endocrinol. 2019; 2019.
  • 8. Sünnetçi Silistre E, Hatipoğl HU. Increased serum circulating asprosin levels in children with obesity. Pediat Int. 2020; 62(4); 467-76.
  • 9. Wang M, Yin C, Wang L, Liu Y, Li H, Li M, et al. Serum asprosin concentrations are increased and associated with insulin resistance in children with obesity. Ann Nutr Metab. 2019; 75(4); 205-212.
  • 10. Zhang L, Chen C, Zhou N, Fu Y, Cheng X. Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride. Clin Chim Acta. 2019; 489; 183-8.
  • 11. Wang Y, Qu H, Xiong X, Qui Y, Liao Y, Chen Y, et al. Plasma asprosin concentrations are increased in individuals with glucose dysregulation and correlated with insulin resistance and first-phase insulin secretion. Mediators Inflamm. 2018; 2018.
  • 12. Gozel N, Kilinc F. Investigation of plasma asprosin and saliva levels in newly diagnosed type 2 diabetes mellitus patients treated with metformin. Endokrynol Pol. 2021; 72(1): 37-43.
  • 13. Deniz R, Yavuzkir S, Ugur K, Ustebay D U, Baykus Y, Ustebay S, et al. Subfatin and asprosin, two new metabolic players of polycystic ovary syndrome. J Obstet Gynaecol. 2021; 41(2): 279-84.
  • 14. Ruth J, Thorben H, Tawfik MYA, Gerhard S, Jörg D, Eva H R, et al. Asprosin in pregnancy and childhood. Mol Cell Pediatr. 2020; 7(18).
  • 15. Baykus Y, Yavuzkir S, Ustebay S, Ugur K, Deniz R, Aydin S. Asprosin in umbilical cord of newborns and maternal blood of gestational diabetes, preeclampsia, severe preeclampsia, intrauterine growth retardation and macrosemic fetus. Peptides. 2019; 120: 170132.
  • 16. Janoschek R, Hoffmann T, Morcos YAT, Sengle G, Dötsch J, Hucklenbruch-Rother E. Asprosin in pregnancy and childhood. Mol Cell Pediatr. 2020; 7(1): 1-5.
  • 17. Mamun M, Hassan MM, Shaikat A H, Islam S A, Hoque M A, Uddin M, et al. Biochemical analysis of blood of native cattle in the hilly area of Bangladesh. Bangladesh J Vet Med. 2013; 11(1): 51-6.
  • 18. Akgul A. Statistical analysis techniques in medical researches “SPSS applications. 3rd ed. Emek Ofset Ltd Sti, Ankara, Turkey; 2005.
  • 19. Anonim. SPSS 22.0 Statistical Package in Social Sciences for Windows. Chicago, USA; 2016.
  • 20. Lee A, Twardock A, Bubar R, Hall J, Davis C. Blood metabolic profiles: their use and relation to nutritional status of dairy cows. J Dairy Sci. 1978; 61(11): 1652-70.
  • 21. Hagawane S, Shinde S, Rajguru D. Haematological and blood biochemical profile in lactating buffaloes in and around Parbhani city. Vet World. 2009; 2(12): 467-9.
  • 22. Tainturier D, Braun J, Rico A, Thouvenot J. Variations in blood composition in dairy cows during pregnancy and after calving. Res Vet Sci. 1984; 37(2): 129-31.
  • 23. Stojević Z, Piršljin J, Milinković-Tur S, Zdelar-Tuk M, Ljubić BB. Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Vet Arh. 2005; 75(1): 67-73.
  • 24. Sakowski T, Kuczyńska B, Puppel K, Metera E, Sloniewski K, Barszczewski J. Relationships between physiological indicators in blood, and their yield, as well as chemical composition of milk obtained from organic dairy cows. J Sci Food Agric. 2012; 92(14): 2905-12.
  • 25. Prodanović R, Sladojević Ž, Kirovski D, Vujanac I, Ivetic V, Savic B, et al. Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. Prodanović R, Sladojević Ž, Kirovski D, et al. Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. Biotechnol Anim Husb. 2012; 28(1): 25-32.
  • 26. Gürgöze S Y, Zonturlu A K, Özyurtlu N, Icen H. Investigation of some biochemical parameters and mineral substance during pregnancy and postpartum period in Awasi ewes. Kafkas Üni Vet Fak Derg. 2009; 15(6): 957-63.
  • 27. Sevinç M, Başoğlu A, Birdane F, Gökçen M, Küçükfındık M. The changes of metabolic profile in dairy cows during dry period and after. Turk J Vet Anim Sci.1999; 23(EK3): 475-8.
  • 28. Li X, Liao M, Shen R, Zhang L, Hu H, Wu J, et al. Plasma asprosin levels are associated with glucose metabolism, lipid, and sex hormone profiles in females with metabolic-related diseases. Mediat Inflamm. 2018; 2018.
  • 29. Zhang X, Jiang H, Ma X, Wu H. Increased serum level and impaired response to glucose fluctuation of asprosin is associated with type 2 diabetes mellitus. J Diabetes Investig. 2020; 11(2): 349-55.
  • 30. Maylem E R S, Spicer L J, Batalha I, Schutz LF. Discovery of a possible role of asprosin in ovarian follicular function. J Mol Endocrinol 2021; 66(1): 35-44.
  • 31. Kilinc M A, Risvanli A. The relationship of asprosin with β-hydroxybutyric acid and postpartum disorders in cows. Acta Vet Hung. Online Publication, 2022.

THE RELATIONSHIP OF BLOOD ASPROSIN LEVELS AND BIOCHEMICAL PARAMETERS IN PREGNANT COWS

Year 2022, , 111 - 116, 28.12.2022
https://doi.org/10.46810/tdfd.1092040

Abstract

In this study, it was aimed to determine asprosin levels in pregnant and non-pregnant cows and to determine the relationship between some biochemical parameters and asprosin levels. For this purpose, 60 Simmental cows were divided into two groups as pregnant (n=30) and non-pregnant cows on the postpartum 15th day (n=30), and asprosin levels and biochemical parameters were measured by taking blood from the cows once. Measured biochemical parameters Alkaline phosphatase (ALP), Amylase, Creatine kinase myocardial band (CK-MB), Creatine kinase N-acetyl cysteine (CK-NAC), Gamma glutamyl transferase (GGT), Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT) ), Direct bilirubin, Total bilirubin , Calcium (Ca), Choline (CHO), Creatine, Glucose, Total protein, Urea, Albumin, Iron, Phosphorus, Lacktate dehydrogenase (LDH-P), High-density lipoprotein cholesterol (HDL-C) ), Low-density lipoprotein (LDL), Triglyceride, Magnesium (Mg) and Unsaturated iron binding capacity (UIBC). Asprosin levels were measured using commercial ELISA kits and biochemical parameters were measured in an autoanalyzer. Asprosin levels and biochemical parameters were compared both for and between groups and the results were evaluated. As a result of the data obtained, the amount of direct bilirubin and total bilirubin in non-pregnant cows was found to be higher than that of pregnant cows, while the amounts of CHO, HDL-C, LDL and asprosin were found to be lower. When asprosin and biochemical parameters of pregnant cows were compared, a positive correlation was found between asprosin and albumin, direct bilirubin, total bilirubin and creatinine levels. In non-pregnant cows, there was a negative correlation between asprosin and amylase and phosphorus, and a positive correlation between asprosin and creatine and glucose. As a result, it was concluded that asprosin levels are higher in pregnant cows and there may be negative or positive correlations between this hormone and biochemical parameters.

References

  • 1. Rossato W, Gonzalez F, Dias M, Ricco D, Valle S F, Rosa V, et al. Number of lactations affects metabolic profile of dairy cows. Arch Vet Sci. 2001; 6(2): 83-8.
  • 2. Payne J, Dew SM, Manston R, Faulks M. The use of a metabolic profile test in dairy herds. Vet Rec. 1970; 87: 150-8.
  • 3. Romere C, Duerrschmid C, Bournat J, Constable P, Jain M, Xia F, et al. Asprosin, a fasting-induced glucogenic protein hormone. Cell 2016; 165(3): 566-79.
  • 4. Alan M, Gurlek B, Yilmaz A, Aksit M, Aslanipour B, Gulhan I, et al. Asprosin: a novel peptide hormone related to insulin resistance in women with polycystic ovary syndrome. Gynecol Endocrinol. 2019; 35(3); 220-3.
  • 5. Duerrschmid C, He Y, Wang C, Li C, Bournat J C, Romero C, et al. Asprosin is a centrally acting orexigenic hormone. Nat Med. 2017; 23(12): 1444-53.
  • 6. Yuan M, Li W, Zhu Y, Yu B, Wu J. Asprosin: a novel player in metabolic diseases. Front Endocrinol. 2020; 11: 1-7.
  • 7. Ugur K, Aydin S. Saliva and blood asprosin hormone concentration associated with obesity. Int J Endocrinol. 2019; 2019.
  • 8. Sünnetçi Silistre E, Hatipoğl HU. Increased serum circulating asprosin levels in children with obesity. Pediat Int. 2020; 62(4); 467-76.
  • 9. Wang M, Yin C, Wang L, Liu Y, Li H, Li M, et al. Serum asprosin concentrations are increased and associated with insulin resistance in children with obesity. Ann Nutr Metab. 2019; 75(4); 205-212.
  • 10. Zhang L, Chen C, Zhou N, Fu Y, Cheng X. Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride. Clin Chim Acta. 2019; 489; 183-8.
  • 11. Wang Y, Qu H, Xiong X, Qui Y, Liao Y, Chen Y, et al. Plasma asprosin concentrations are increased in individuals with glucose dysregulation and correlated with insulin resistance and first-phase insulin secretion. Mediators Inflamm. 2018; 2018.
  • 12. Gozel N, Kilinc F. Investigation of plasma asprosin and saliva levels in newly diagnosed type 2 diabetes mellitus patients treated with metformin. Endokrynol Pol. 2021; 72(1): 37-43.
  • 13. Deniz R, Yavuzkir S, Ugur K, Ustebay D U, Baykus Y, Ustebay S, et al. Subfatin and asprosin, two new metabolic players of polycystic ovary syndrome. J Obstet Gynaecol. 2021; 41(2): 279-84.
  • 14. Ruth J, Thorben H, Tawfik MYA, Gerhard S, Jörg D, Eva H R, et al. Asprosin in pregnancy and childhood. Mol Cell Pediatr. 2020; 7(18).
  • 15. Baykus Y, Yavuzkir S, Ustebay S, Ugur K, Deniz R, Aydin S. Asprosin in umbilical cord of newborns and maternal blood of gestational diabetes, preeclampsia, severe preeclampsia, intrauterine growth retardation and macrosemic fetus. Peptides. 2019; 120: 170132.
  • 16. Janoschek R, Hoffmann T, Morcos YAT, Sengle G, Dötsch J, Hucklenbruch-Rother E. Asprosin in pregnancy and childhood. Mol Cell Pediatr. 2020; 7(1): 1-5.
  • 17. Mamun M, Hassan MM, Shaikat A H, Islam S A, Hoque M A, Uddin M, et al. Biochemical analysis of blood of native cattle in the hilly area of Bangladesh. Bangladesh J Vet Med. 2013; 11(1): 51-6.
  • 18. Akgul A. Statistical analysis techniques in medical researches “SPSS applications. 3rd ed. Emek Ofset Ltd Sti, Ankara, Turkey; 2005.
  • 19. Anonim. SPSS 22.0 Statistical Package in Social Sciences for Windows. Chicago, USA; 2016.
  • 20. Lee A, Twardock A, Bubar R, Hall J, Davis C. Blood metabolic profiles: their use and relation to nutritional status of dairy cows. J Dairy Sci. 1978; 61(11): 1652-70.
  • 21. Hagawane S, Shinde S, Rajguru D. Haematological and blood biochemical profile in lactating buffaloes in and around Parbhani city. Vet World. 2009; 2(12): 467-9.
  • 22. Tainturier D, Braun J, Rico A, Thouvenot J. Variations in blood composition in dairy cows during pregnancy and after calving. Res Vet Sci. 1984; 37(2): 129-31.
  • 23. Stojević Z, Piršljin J, Milinković-Tur S, Zdelar-Tuk M, Ljubić BB. Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Vet Arh. 2005; 75(1): 67-73.
  • 24. Sakowski T, Kuczyńska B, Puppel K, Metera E, Sloniewski K, Barszczewski J. Relationships between physiological indicators in blood, and their yield, as well as chemical composition of milk obtained from organic dairy cows. J Sci Food Agric. 2012; 92(14): 2905-12.
  • 25. Prodanović R, Sladojević Ž, Kirovski D, Vujanac I, Ivetic V, Savic B, et al. Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. Prodanović R, Sladojević Ž, Kirovski D, et al. Use of metabolic profiles and body condition scoring for the assessment of energy status of dairy cows. Biotechnol Anim Husb. 2012; 28(1): 25-32.
  • 26. Gürgöze S Y, Zonturlu A K, Özyurtlu N, Icen H. Investigation of some biochemical parameters and mineral substance during pregnancy and postpartum period in Awasi ewes. Kafkas Üni Vet Fak Derg. 2009; 15(6): 957-63.
  • 27. Sevinç M, Başoğlu A, Birdane F, Gökçen M, Küçükfındık M. The changes of metabolic profile in dairy cows during dry period and after. Turk J Vet Anim Sci.1999; 23(EK3): 475-8.
  • 28. Li X, Liao M, Shen R, Zhang L, Hu H, Wu J, et al. Plasma asprosin levels are associated with glucose metabolism, lipid, and sex hormone profiles in females with metabolic-related diseases. Mediat Inflamm. 2018; 2018.
  • 29. Zhang X, Jiang H, Ma X, Wu H. Increased serum level and impaired response to glucose fluctuation of asprosin is associated with type 2 diabetes mellitus. J Diabetes Investig. 2020; 11(2): 349-55.
  • 30. Maylem E R S, Spicer L J, Batalha I, Schutz LF. Discovery of a possible role of asprosin in ovarian follicular function. J Mol Endocrinol 2021; 66(1): 35-44.
  • 31. Kilinc M A, Risvanli A. The relationship of asprosin with β-hydroxybutyric acid and postpartum disorders in cows. Acta Vet Hung. Online Publication, 2022.
There are 31 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Articles
Authors

Mehmet Akif Kılınç 0000-0003-1577-1556

Ali Rişvanlı 0000-0001-5653-0025

Tarık Şafak 0000-0002-6178-4641

Öznur Yılmaz 0000-0003-0424-9471

Burak Fatih Yüksel 0000-0002-7256-9189

İbrahim Şeker 0000-0002-3114-6411

Publication Date December 28, 2022
Published in Issue Year 2022

Cite

APA Kılınç, M. A., Rişvanlı, A., Şafak, T., Yılmaz, Ö., et al. (2022). GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ. Türk Doğa Ve Fen Dergisi, 11(4), 111-116. https://doi.org/10.46810/tdfd.1092040
AMA Kılınç MA, Rişvanlı A, Şafak T, Yılmaz Ö, Yüksel BF, Şeker İ. GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ. TDFD. December 2022;11(4):111-116. doi:10.46810/tdfd.1092040
Chicago Kılınç, Mehmet Akif, Ali Rişvanlı, Tarık Şafak, Öznur Yılmaz, Burak Fatih Yüksel, and İbrahim Şeker. “GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ”. Türk Doğa Ve Fen Dergisi 11, no. 4 (December 2022): 111-16. https://doi.org/10.46810/tdfd.1092040.
EndNote Kılınç MA, Rişvanlı A, Şafak T, Yılmaz Ö, Yüksel BF, Şeker İ (December 1, 2022) GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ. Türk Doğa ve Fen Dergisi 11 4 111–116.
IEEE M. A. Kılınç, A. Rişvanlı, T. Şafak, Ö. Yılmaz, B. F. Yüksel, and İ. Şeker, “GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ”, TDFD, vol. 11, no. 4, pp. 111–116, 2022, doi: 10.46810/tdfd.1092040.
ISNAD Kılınç, Mehmet Akif et al. “GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ”. Türk Doğa ve Fen Dergisi 11/4 (December 2022), 111-116. https://doi.org/10.46810/tdfd.1092040.
JAMA Kılınç MA, Rişvanlı A, Şafak T, Yılmaz Ö, Yüksel BF, Şeker İ. GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ. TDFD. 2022;11:111–116.
MLA Kılınç, Mehmet Akif et al. “GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ”. Türk Doğa Ve Fen Dergisi, vol. 11, no. 4, 2022, pp. 111-6, doi:10.46810/tdfd.1092040.
Vancouver Kılınç MA, Rişvanlı A, Şafak T, Yılmaz Ö, Yüksel BF, Şeker İ. GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ. TDFD. 2022;11(4):111-6.