A NEW METHOD FOR OBTAINING HAPLOID PLANT SHED-MICROSPORTS CULTURE

Abstract

In plant breeding studies, dihaploidization method is used to obtain homozygous pure lines used to develop varieties in a shorter time. Dihaploidization method is applied in most types of vegetables such as wheat and corn, as well as in vegetable species. Androgenesis methods (anther and microspore culture) and gynogenesis and parthenogenesis methods (ovula and ovary culture) are used to obtain haploid plants. One of the androgenetic methods, shed-microspore culture has been a new alternative technique to anther and microspore cultures, and successful results have been obtained. The most important feature of this technique is the presence of a double-layered nutrient medium in a single petri dish, and the culture of microspores in the anthers in solid and liquid nutrient medium. In this study, the definition and application of microspore culture and shed-microspore cultures, studies conducted in the world and in our country and their developments in recent years were examined.

Keywords

• pepper,
• shed-microspore culture,
• androgenesis,

References

1. Andrews, J. (1985). Peppers, The Domesticated Capsicum. University of Texas Press, Austin, Texas. Bal, U., Abak, K., Büyükalaca, S., Çömekçioğlu, N. 2003. Development of Callus Colonies From the Isolated Microspore Culture of Capsicum annuum L. Biotechnol. & Biotechnol. Eq., 17(2): 28-43.
2. Khush, G. S. and Virmani, S. S., 1996. Somatic embriyogenesis in cultured unfertilized ovules of Cucurbita moschata. Journal of the Japanese Society for Horticultural Science,57 (1):34-42.
3. Çömlekçioğlu, N., Büyükalaca, S. ve Abak, K., 2001. Effect of Silver Nitrate on Haploid Embryo Induction by Anther Culture in Pepper (Capsicum annuum L.). XI. th. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, April 9-13, Antalya.
4. Ellialtıoğlu, Ş. ve Tıpırdamaz, R. 2002. Soğuk uygulamaları ve aktif kömürün biberde (Capsicum annuum L.) Anter kültürü süresince absisik asit miktarındaki değişim üzerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi,15 (1): 9-18.
5. Ellialtıoğlu, S., Kaplan F., and Abak, K., 2001. The effect of carrot extract and activated charcoal on the androgenesis of pepper (K. Abak., S. Büyükalaca, Y. Daşgan editors). Proc. of the XIth EUCARPIA Meeeting on Genetics and Breeding of Capsicum & Eggplant, 9-13 April 2001, Antalya, Turkey, pp. 142-145.
6. Popova, T., Grozeva, S., Todorova, V., Stankova, G., Anachkov, N. and Rodeva, V., 2016. Effects of low temperature, genotype and culture media on in vitro androgenic answer of pepper (Capsicum annuum L). Acta Physiologiae Plantarum,38, 273.
7. Parra-Vega V. and Seguí-Simarro J. M., 2016. Anther Culture in Pepper (Capsicum annuum L.). Methods in Molecular Biology (pp. 1359). New York, NY.
8. Ozsan, T. and Onus, A. N., 2017. In vitro biber (Capsicum annuum L.) anter kültürü B vitaminleri ile etkilenebilir mi? Uluslararası Biyoteknoloji Dergisi,20(1):1-13.

9. Bal, U., 2002. Domateste (Lycopersicon esculentum Mill.) Izole Edilmiş Mikrospor Kültürü,Ovaryum Kültürü ve Solanum sisymbriifolium Lam. ile Tozlanma Yöntemleri ile Haploid Embriyo Oluşumunun Uyartılması. T.Ü. Tekirdağ Ziraat Fakültesi BahçeBitkileri Anabilim Dalı, Doktora Tezi (basılmamış),1-156 s., Tekirdağ.
10. Nitsch, C., Norreel, K., 1973. Effect d’un choc thermique sur le pouvoir embryogene du polende Datura innoxia cultivé dans l’anthere ou isolé de l’anthere. C.R. Acad. Sci., 276 D, 303-306.
11. Lichter, B., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z.Pflanzenphysiol. 105: 427-434.
12. Takahata, Y., Keller, W.A., 1991. High Frequency Embryo Genesis and Plant Regeneration inIsolated Microspore Culture of Brassica oleracea L. Plant Science, 74: 235-242.
13. Duijs, J.G., Voorrips, R.E., Visser, D.L., Custers, J.B.M., 1992. Microspore Culture isSuccessful In Most Crop Types of Brassica oleracea L. Euphytica, 60: 45-55.
14. Cao, M., Li, Y., Liu, F., Dore, C., 1994. Embryo Genesis and Plant Regeneration of Pakchoi (B.rapa L.ssp. chinensis) via in vitro Isolated Microspore Culture. Plant Cell Reports, 13: 447-450.
15. Holme, I.B., Olesen, A., Hansen N.J.P., Andersen, S.B., 1999. Anther and IsolatedMicrospore Culture Response of Wheat Lines from Northwestern and Eastern Europe. Plant Breeding, 118: 111-117.
16. Barro, F., Martin, A., 1999. Response of Different Genotype of Brassica carinata to Microspore culture. Plant Breeding, 118: 79-81.
17. Nageli, M., Schmid, J.E., Stamp, P., Büter, B., 1999. Improved Formation of RegenerableCallus in Isolated Microspore Culture of Maize: Impact of Carbohydrates, Plating Density and Time of Transfer. Plant Cell Reports, 19: 177-184.
18. Ferrie, A.M.R., Taylor, D.C., Mackenzie, S.L., Keller, W.A., 1999. Microspore embryo genesisof high sn-2 erucic acid Brassica oleracea germplazm. Plant Cell, Tissue and Organ Culture, 57: 79-84.
19. Lemonnier-Le Penhuizic, C., Chatelet, C., Kloareg, B., Potin, P., 2001. Carrageenan Oligosaccarides Enhance Stress-Induced Microspore Embryo Genesis in Brassicaoleracea var. italica. Plant Science, 160: 1211-1220.
20. Kasha, K.J., Hu, T.C., Oro, R., Simion, E., Shim, Y.S., 2001. Nuclear Fusion Leads toChromosome Doubling During Mannitol Pretreatment of Barley (Hordeum vulgare L.) Microspores. Journal of Experimental Botany, Vol. 52, No. 359, pp. 1227-1238.
21. Dias, J.S., Correia, M.C., 2002. Effect of Medium Renovation and Incubation TemperatureRegimes on Tronchuda Cabbage Microspore Culture Embryo Genesis. Scientia Horticulturae, 93: 205-214.
22. Segui-Simarro, J.M., Testillano, P.S., Risueno, M.C., 2003. Hsp70 and Hsp90 Change TheirExpression and Subcellular Localization After Microspore Embryo Genesis Induction in Brassica napus L. Journal of Structural Biology, 142: 379-381.
23. Bal, U., Abak, K., Büyükalaca, S., Çömekçioğlu, N. 2003. Development of CallusColonies From the Isolated Microspore Culture of Capsicum annuum L.Biotechnol. & Biotechnol. Eq., 17(2): 28-43.
24. Patel, M., Darvey, N.L., Marshall, D.R. and Berry, J. O. 2004. Optimization of culture conditions for improved plant regeneration efficiencyfrom wheat microspore culture. Euphytica, 140: 197-204.
25. Weber, S., Ünker, F., Friedt, W., 2005. Improved Doubled Haploid Production Protocol forBrassica napus Using Microspore Colchicine Treatment in vitro and Ploidy Determination by Flow Cytometry. Plant Breeding, 124: 511-513.
26. Dunwell, J. M. 2010. Haploids in flowering plants: origins and exploitation. PlantBiotechnology Journal, 8: 377-424.
27. Lantos, C., Juhasz, A.G., Vagi, P., Mihaly, R., Kristof, Z., Pauk, J. 2012. Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). PlantBiotechnology Rep, 6:123-132.
28. Nitsch, C., Nitsch, J.P., 1967. The Induction of Flowering in vitro in Stem Segments ofPlumbago indica L. I. The Production of Vegetative Buds. Planta, 72, 355-370.
29. Gamborg, O.L., Miller, R.A., Ojima, K., 1968. Nutrient Requirements of Suspension Culturesof Soybean Root Cells. Exp. Cell Res., 60: 151-158.
30. Lichter, B., 1981. Anther culture of Brassica napus in A Liquid Culture Medium. Z.Pflanzenphysiol. 103: 229-237.
31. Kyo, M., Harada, H., 1986. Control of the Developmental Pathway of Tobacco Pollen in vitro.Planta, 168: 427-432.
32. Duijs, J.G., Voorrips, R.E., Visser, D.L., Custers, J.B.M., 1992. Microspore Culture isSuccessful In Most Crop Types of Brassica oleracea L. Euphytica, 60: 45-55.
33. Carlos, J., Dias, S., 1999. Effect of Activated Charcoal on Brassica oleracea MicrosporeCulture Embryo Genesis. Euphytica, 108: 65-69.
34. Indrianto, A., Heberle-Bors, E., Touraev, A., 1999. Assessment of Various Stresses andCarbohydrates for Their Effect on the Induction of Embryo Genesis in Isolated Wheat Microspores. Plant Science, 143:71-79.
35. Jahne-Gartner, A., Lorz, H.1999 Protocols for Anther and Microspore Culture of Barley.Methods Mol. Biol., 111: 269-279.
36. Supena, E. D. J., Suharsono, S., Jacobsen E., Custers, J. B. M., 2006a. Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.)Plant Cell Rep,25: 1–10 DOI 10.1007/s00299-005-0028-y.
37. Arı, E. 2006. Anemone coronaria var. in coccinea anther culture studies, PhD Thesis, Çukurova University, Science Institute, Department of Horticulture, Adana.
38. Coşkun, Y. 2011. Microspore culture and durum wheat plant (Triticum status Desf.) embryo production and plant regeneration, PhD Thesis, Süleyman Demirel University, Graduate School of Natural and Applied Sciences, Department of Biology Branch, Isparta.
39. Yin, T., Tian, S., Luo, S., Chen, X., Wang, Y., Shen, S. 2010. Studies on isolatedmicrospore embryoid induction of C. annuum L. Front. Agric. China, 4(4): 438-442.
40. Dumas, De Vaulx, R., Chambonnet, D. & Pochard, E., 1981. In vitro culture of pepper (Capsicum annuum L.) anthers: high rate plant production from different genotypes by +35 0C treatments. Agronomie,1(10):859-864.
41. Abak, K., 1983. Biberde (Capsicum annuum L.) Anter Kültürü Yoluyla Haploid Bitki Elde Etme Üzerinde Araştırma. Ankara Üniversitesi Ziraat Fakültesi Yıllığı. Cilt: 33, Fasikül 1-2-3-4’den ayrı basım, 155-163.
42. Lantos, C., Juhasz, A.G., Somogyi, G., Ötvös, K., Vagi, P., Mihaly, R., Kristof, Z.,Somogyi, N., Pauk, J. 2009. Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat. PlantCell Tissue Organ Culture, 97: 285-293.
43. Kim, M., Jang, I.C., Kim, J.A., Park, E.J., Yoon, M., Lee, Y. 2008. Embriyogenesis andplant regeneration of hot pepper (Capsicum annuum L.) through isolatedmicrospore culture. Plant Cell Rep, 27: 425- 434.
44. Supena, E. D. J. ve Custers, J. B. M., 2002. Establishment of haploid technology to improve hot pepper breeding efficiency in Indonesia. Proceedings of the International 7th Indonesian Student’s Scientific Meeting, ISSM 2002. Berlin, October 4-6, 69-72.
45. Supena, E.D.J., Muswita, W. and Suharsono, S., 2006b. Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.). Scienta Horticulturae,107: 226-232.
46. Supena, E. D. J., Custers, J. B. M., 2011. Refinement of shed-microspore culture protocol to increase normal embryos production in hot pepper (Capsicum annuum L.). Scientia Horticulturae,130 (2011) 769–774.
47. Supena, E. D. J., 2004. Innovations in microspore embryogenesis in Indonesian hotpepper (Capsicum annuum L.) and Brassica napus L. (phd thesis). Wageningen University, 131.
48. Erim, F. B., 2019. Farklı Biber (Capsicum Annuum L.) Genotipleri Üzerinde Shedmikrospor Kültürü Tekniğinin Uygulanması ve Mikrospor Gelişim Aşamalarının Belirlenmesi (yüksek lisans tezi). Akdeniz Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı, Antalya.
49. Benli, Y., 2019. A Study on the Response of Some Pepper (Capsicum annuum L.) Genotypes to Different Androgenesis Methods (master's thesis). Akdeniz University, Institute of Science, Department of Horticulture, Antalya.
50. Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum,15 (3): 473-497.
51. Doğan, S., Arı, E., 2019. The effect of shaker use on embryo yield in shed-microspore cultures of ornamental peppers. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi (UTYHBD), 5 (2): 299-307.
52. Tatar, M., 2022. Gaziantep yerel biber genotiplerinin morfolojik ve moleküler karakterizasyonu ve double haploid (DH) hatların elde edilme olanakları (Doktora tezi). Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı, Van.