The Antinociceptive Effect of Adalimumab, a TNF-Alpha Inhibitor, in a Mice Model of Inflammatory Pain

Abstract

The aim of this study, evaluate the nociceptive effect of adalimumab, a TNF-alpha inhibitor, on formalin-induced inflammatory pain in mice. The antinociceptive activity of adalimumab was determined on adult male BALB-C mice (6 mice in each group) by a formalin-induced inflammatory pain model and hot plate test applied. Diclofenac, which we used as a positive control, showed anti-nociceptive activity in both phases of the formalin test. The effect of the hot plate test at the thirtieth minute was statistically significantly different. Adalimumab was not effective in the neuronal phase of the formalin test but showed an antinociceptive effect in the inflammatory phase. Changes due to adalimumab in the hot plate test did not reach statistical significance. The findings of this study showed that adalimumab, which we applied as a single dose, had antinociceptive activity in the inflammatory phase of the formalin test. This result indicated that the peripheral analgesic effect of adalimumab is stronger.

Keywords

• Adalimumab
• TNF-alpha
• formalin test
• inflammatory pain
• hot plate test

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