The Role of Royal Jelly on Exhaustive Exercise-Induced Oxidative Stress

Abstract

In this study, the effects of Royal Jelly (RJ) on oxidative stress caused by exhaustive swimming exercise in rat tissues were evaluated. Methods: Twenty four male Wistar albino rats were indiscriminately distributed into four experimental groups: Sedentary control (SC); SC with administration of RJ (100 mg kg-1) (SC + RJ); exhaustive swimming exercise (E); Exhaustive swimming exercise with administration of RJ (100 mg kg-1) (E + RJ). 100 mg kg-1 of RJ were dissolved in drinking water. Rats in the SC+RJ and E+RJ groups supplemented with RJ (100 mg kg-1) orally once a day for two weeks. Rats in groups E and E+RJ subjected to acute exhaustive swimming exercise on the 14th day of the study, then some biochemical parameters related to oxidative stress of all groups were measured. Results: The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine (CRE) levels significantly raised in the exercised rats compared with the sedentary rats (P < 0.05). The decreased superoxide dismutase (SOD), glutathione peroxidase (GSHPx), and catalase (CAT) activities of muscular and hepatic tissues significantly increased and the high malondialdehyde (MDA) levels of muscular, hepatic and kidney tissues significantly reduced in exercised rats treated with RJ (P < 0.05). Conclucion: Collectively, in this study, protective effects of RJ on tissues against oxidative damage after exhaustive exercise were observed.

Keywords

• Royal Jelly
• Liver
• Kidney
• Skeletal muscle
• Exhaustive exercise
• Antioxidant

Arı Sütünün Tüketici Egzersize Bağlı Oksidatif Stres Üzerindeki Rolü

Abstract

Bu çalışmada, Arı Sütü (RJ)'nin sıçan dokularındaki tüketici yüzme egzersizinin neden olduğu oksidatif stres üzerindeki etkileri değerlendirildi. Metodlar: Yirmi dört adet erkek Wistar albino sıçan rastgele dört deney grubuna ayrıldı: Sedanter kontrol (SC); 100 mg kg-1 RJ uygulanmış SC (SC + RJ); tüketici yüzme egzersiz grubu (E); 100 mg kg-1 RJ uygulanmış tüketici yüzme egzersiz grubu (E + RJ). 100 mg kg-1 RJ içme suyunda çözüldü. SC + RJ ve E + RJ gruplarındaki sıçanlara, iki hafta boyunca günde bir kez ağızdan RJ (100 mg kg-1) verildi. E ve E + RJ gruplarındaki sıçanlar çalışmanın 14. gününde akut tüketici yüzme egzersizine tabi tutulduktan sonra tüm grupların oksidatif stres ile ilgili bazı biyokimyasal parametreleri ölçüldü. Bulgular: Alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), alkalin fosfataz (ALP), kan üre azotu (BUN) ve kreatinin (CRE) seviyelerinin aktiviteleri, egzersiz yapılan sıçanlarda, sedanter sıçanlara kıyasla önemli ölçüde artmıştır (P < 0.05). RJ ile tedavi edilen sıçanlarda kas ve hepatik dokuların azalmış süperoksit dismutaz (SOD), glutatyon peroksidaz (GSHPx) ve katalaz (CAT) aktiviteleri önemli ölçüde artmış ve kas, hepatik ve böbrek dokularının yüksek malondialdehit (MDA) seviyeleri önemli ölçüde azalmıştır (P < 0.05). Sonuç: Sonuç olarak, bu çalışmada, tüketici egzersiz sonrası oluşan dokular üzerindeki oksidatif hasara karşı RJ'nin koruyucu etkileri gözlenmiştir.

Keywords

• Arı sütü
• Karaciğer
• Böbrek
• İskelet kası
• Tüketici egzersiz
• Antioksidan

References

1. Moore SC, Lee I-M, Weiderpass E, Campbell PT, Sampson JN, Kitahara CM, et al. Association of leisure-time physical activity with risk of 26 types of cancer in 1.44 million adults. JAMA internal medicine. 2016;176(6):816-25.
2. Morillas-Ruiz JM, Hernández-Sánchez P. Oxidative stress and antioxidant defenses induced by physical exercise. Basic principles and clinical significance of oxidative stress Rijeka: InTechOpen. 2015:221-41.
3. Malaguti M, Angeloni C, Garatachea N, Baldini M, Leoncini E, Collado PS, et al. Sulforaphane treatment protects skeletal muscle against damage induced by exhaustive exercise in rats. Journal of Applied Physiology. 2009;107(4):1028-36.
4. Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological reviews. 2008;88(4):1243-76.
5. Halliwell B, Gutteridge JM. Free radicals in biology and medicine: Oxford university press, USA; 2015.
6. Liu Z, Ren Z, Zhang J, Chuang C-C, Kandaswamy E, Zhou T, et al. Role of ROS and nutritional antioxidants in human diseases. Frontiers in physiology. 2018;9:477.
7. Estruel-Amades S, Massot-Cladera M, Garcia-Cerdà P, Pérez-Cano FJ, Franch À, Castell M, et al. Protective effect of hesperidin on the oxidative stress induced by an exhausting exercise in intensively trained rats. Nutrients. 2019;11(4):783.
8. Gholamian-Dehkordi N, Luther T, Asadi-Samani M, Mahmoudian-Sani MR. An overview on natural antioxidants for oxidative stress reduction in cancers; a systematic review. Immunopathologia Persa. 2017;3(2):e12.

9. Chatterjee M, Saluja R, Kanneganti S, Chinta S, Dikshit M. Biochemical and molecular evaluation of neutrophil NOS in spontaneously hypertensive rats. Cellular and Molecular Biology. 2007;53(1):84-93.
10. Sies H. Oxidative stress: a concept in redox biology and medicine. Redox biology. 2015;4:180-3.
11. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxidative medicine and cellular longevity. 2017;2017.
12. Liu Z, Zhou T, Ziegler AC, Dimitrion P, Zuo L. Oxidative stress in neurodegenerative diseases: from molecular mechanisms to clinical applications. Oxidative medicine and cellular longevity. 2017;2017.
13. Atashak S. The antioxidant role of artichoke (Cynara scolymus L.) extract against exhaustive exercise-induced oxidative stress in young athletes. Journal of Medicinal Plants. 2019;18(71):37-48.
14. Bărnuţiu LI, Mărghitaş LA, Dezmirean DS, Mihai CM, Bobiş O. Chemical composition and antimicrobial activity of Royal Jelly-REVIEW. Scientific Papers Animal Science and Biotechnologies. 2011;44(2):67-72.
15. Kohno K, Okamoto I, Sano O, Arai N, Iwaki K, Ikeda M, et al. Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Bioscience, biotechnology, and biochemistry. 2004;68(1):138-45.
16. Bengü AŞ, Adnan A, Özbolat S, Abdullah T, Aykutoğlu G, Çiftçİ M, et al. Content and antimicrobial activities of bingol royal jelly. Türk Tarım ve Doğa Bilimleri Dergisi. 2020;7(2):480-6.
17. Asadi N, Kheradmand A, Gholami M, Saidi SH, Mirhadi SA. Effect of royal jelly on testicular antioxidant enzymes activity, MDA level and spermatogenesis in rat experimental Varicocele model. Tissue and Cell. 2019;57:70-7.
18. Ghanbari E, Nejati V, Khazaei M. Improvement in serum biochemical alterations and oxidative stress of liver and pancreas following use of royal jelly in streptozotocin-induced diabetic rats. Cell Journal (Yakhteh). 2016;18(3):362.
19. Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of royal jelly in cisplatin-induced testes damage. Urology. 2009;74(3):545-51.
20. Zhang H, Liu M, Zhang Y, Li X. Trimetazidine attenuates exhaustive exercise-induced myocardial injury in rats via regulation of the Nrf2/NF-κB signaling pathway. Frontiers in pharmacology. 2019;10:175.
21. Thomas D, Marshall K. Effects of repeated exhaustive exercise on myocardial subcellular membrane structures. International journal of sports medicine. 1988;9(04):257-60.
22. Ji LL, Kang C, Zhang Y. Exercise-induced hormesis and skeletal muscle health. Free Radical Biology and Medicine. 2016;98:113-22.
23. de Sousa CV, Sales MM, Rosa TS, Lewis JE, de Andrade RV, Simões HG. The antioxidant effect of exercise: a systematic review and meta-analysis. Sports medicine. 2017;47:277-93.
24. Radak Z, Ishihara K, Tekus E, Varga C, Posa A, Balogh L, et al. Exercise, oxidants, and antioxidants change the shape of the bell-shaped hormesis curve. Redox biology. 2017;12:285-90.
25. Di Meo S, Napolitano G, Venditti P. Mediators of physical activity protection against ROS-linked skeletal muscle damage. International journal of molecular sciences. 2019;20(12):3024.
26. Nocella C, Cammisotto V, Pigozzi F, Borrione P, Fossati C, D’Amico A, et al. Impairment between oxidant and antioxidant systems: short-and long-term implications for athletes’ health. Nutrients. 2019;11(6):1353.
27. Elejalde E, Villarán MC, Alonso RM. Grape polyphenols supplementation for exercise-induced oxidative stress. Journal of the International Society of Sports Nutrition. 2021;18(1):3.
28. Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or foe? Journal of sport and health science. 2020;9(5):415-25.
29. Popovic LM, Mitic NR, Radic I, Miric D, Kisic B, Krdzic B, et al. The effect of exhaustive exercise on oxidative stress generation and antioxidant defense in guinea pigs. Advances in Clinical and Experimental Medicine. 2012;21(3):313-20.
30. Vale A, Ferreira H, Benetti E, Rebelo A, Figueiredo A, Barbosa E, et al. Antioxidant effect of the pequi oil (Caryocar brasiliense) on the hepatic tissue of rats trained by exhaustive swimming exercises. Brazilian Journal of Biology. 2018;79:257-62.
31. Huang Q, Ma S, Tominaga T, Suzuki K, Liu C. An 8-week, low carbohydrate, high fat, ketogenic diet enhanced exhaustive exercise capacity in mice part 2: effect on fatigue recovery, post-exercise biomarkers and anti-oxidation capacity. Nutrients. 2018;10(10):1339.
32. Huang K-C, Wu W-T, Yang F-L, Chiu Y-H, Peng T-C, Hsu B-G, et al. Effects of freshwater clam extract supplementation on time to exhaustion, muscle damage, pro/anti-inflammatory cytokines, and liver injury in rats after exhaustive exercise. Molecules. 2013;18(4):3825-38.
33. Kanbur M, Eraslan G, Beyaz L, Silici S, Liman BC, Altınordulu Ş, et al. The effects of royal jelly on liver damage induced by paracetamol in mice. Experimental and Toxicologic Pathology. 2009;61(2):123-32.
34. Huang C-C, Lin T-J, Lu Y-F, Chen C-C, Huang C-Y, Lin W-T. Protective effects of L-arginine supplementation against exhaustive exercise-induced oxidative stress in young rat tissues. Chin J Physiol. 2009;52(5):306-15.
35. Aksoy L, Aslan Z. Nephroprotective and antioxidative effects of royal jelly on ethylene glycol induced nephropathy in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017;64(4):241-8.
36. Yan F, Hao H. Effects of Laminaria japonica polysaccharides on exercise endurance and oxidative stress in forced swimming mouse model. Journal of Biological Research-Thessaloniki. 2016;23(1):1-7.
37. Yang Q, Jin W, Lv X, Dai P, Ao Y, Wu M, et al. Effects of macamides on endurance capacity and anti-fatigue property in prolonged swimming mice. Pharmaceutical biology. 2016;54(5):827-34.
38. Yan F, Wang B, Zhang Y. Polysaccharides from Cordyceps sinensis mycelium ameliorate exhaustive swimming exercise-induced oxidative stress. Pharmaceutical Biology. 2014;52(2):157-61.
39. Kruk J, Aboul-Enein BH, Duchnik E, Marchlewicz M. Antioxidative properties of phenolic compounds and their effect on oxidative stress induced by severe physical exercise. The Journal of Physiological Sciences. 2022;72(1):1-24.