Investigation of Some Metabolic Enzyme Activities in Samples of Serum and Humor Aqueous of Cataract Cases with Pseudoexfoliation Syndrome

Abstract

Objective: Pseudoexfoliation syndrome (PEX) occurs as a result of aggregation of extracellular matrix material in ocular tissues and visceral organs and it is known as a systemic disease. The purpose of our study is to investigate the activities of carbonic anhydrase (CA), glutathione reductase (GR), aldose reductase (AR), paraoxonase (PON) enzymes in aqueous and serum samples, also serum selenium levels. Methods: Serum and aqueous samples from 33 PEX and 33 control subjects were collected. Enzyme activities were studied in both serum and aqueous samples by biochemical methods. Results: According to the results, serum CA hydratase enzyme activity levels were higher in the samples with PEX compared to the control samples (p < 0.05). Aqueous CA hydratase and aqueous/serum PON, GR and AR activity levels did not differ significantly among the study samples (p > 0.05). Conclusions: Based on the relationship of carbonic anhydrase with aqueous production and glaucoma, increased CA hydratase activity may help to understand the etiopathogenesis and mechanism of conversion to glaucoma of PEX. In the literature, we did not find a study on activity of CA in aqueous and serum samples in patients with PEX. Therefore, we believe that this study can be a reference.

Keywords

• Pseudoexfoliation syndrome
• carbonic anhydrase
• aldose reductase
• glutathione reductase
• paraoxonase

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