Effects of thymoquinone against D- galactose and aluminum chloride induced testicular dysfunction in rats.

Abstract

Alzheimer's disease affects all tissues negatively. In this study, it was aimed to determine the effects of TQ on testicular toxicity in rats with experimental Alzheimer's disease. In the study, 33 Wistar Albino rats weighing 190-230 gr were used. Rats were 11 rats in each group; were divided into 3 equal groups as control, ALZ and ALZ+TQ. The control group was given saline by IP route for 28 days daily. The ALZ group was given 60 mg/kg D-gal + 40 mg/kg AlCl3 IP for 28 days daily. The ALZ+TQ group was given 60 mg/kg D-gal + 40mg/kg AlCl3 for 28 days by IP + 20 mg/kg TQ by oral gavage for last 14 days daily. According to the findings of our study, a decrease in SOD, CAT, GPx activity and GSH levels and an increase in MDA levels were determined in the ALZ group. In the ALZ+TQ group, while SOD, GPx, CAT activities and GSH levels increased, MDA levels decreased. Semen analyzes showed that there was a decrease in sperm motility and sperm density in the ALZ group, and an increase in the rate of abnormal sperm and dead spermatozoa. In the ALZ+TQ group, sperm motility, abnormal sperm rate and dead spermatozon rate improved. As a result, it was determined that the decrease in semen quality and increase in oxidative stress induced by AlCl3+D-Gal were suppressed by TQ, protecting the testicular tissue from oxidative damage and increasing semen quality.

Keywords

• Alzheimer
• AlCl3+ D-Galactose
• oxidative stress
• rat
• sperm
• thymoquinone.

Timokinonun sıçanlarda D-galaktoz ve alüminyum klorür kaynaklı testis fonksiyon bozukluğuna karşı etkileri.

Öz

Alzheimer hastalığı tüm dokularda olumsuz etkilemektedir. Bu çalışmada deneysel alzheimer hastalığı oluşturulan ratlarda testis toksisitesi üzerine TQ etkileri belirlenmesi amaçlanmıştır. Çalışmada ağırlıkları 190-230 gr arasında değişen 33 adet Wistar Albino cinsi rat kullanıldı. Sıçanlar, her grupta 11 sıçan olmak üzere kontrol, ALZ ve ALZ+TQ olarak 3 eşit gruba ayrıldı. Kontrol grubuna, 28 gün boyunca IP yoluyla serum fizyolojik günlük olarak verildi. ALZ grubuna 28 gün boyunca günlük olarak 60 mg/kg D-gal+40 mg/kg AlCl3 IP verildi. ALZ+TQ grubuna 28 gün 60 mg/kg D-gal +40mg/kg AlCl3 IP+20 mg/kg TQ son 14 gün oral sonda ile günlük olarak verildi. Çalışmamızın bulgularına göre ALZ grubunda SOD, CAT, GPx aktivitesi ve GSH düzeylerinde azalma, MDA düzeylerinde ise artış saptanmıştır. ALZ+TQ grubunda SOD, GPx, CAT aktiviteleri ve GSH seviyeleri artarken MDA seviyeleri azaldı. Sperma analizleri, ALZ grubunda sperm motilite ve sperm yoğunluğunda azalma, anormal sperm ve ölü canlı spermatozoa oranında artış olduğunu göstermiştir. ALZ+TQ tedavi grubunda sperm hareketliliği, anormal sperm oranı ve ölü sperm oranı düzeldi. Sonuç olarak, AlCl3+D-Gal'in neden olduğu semen kalitesindeki azalma ve oksidatif stres artışının TQ tarafından baskılandığı, testis dokusunu oksidatif hasardan koruduğu ve semen kalitesini arttırdığı belirlendi.

Anahtar Kelimeler

• Alzheimer
• AlCl3+D-Galaktoz
• oksidatif stres
• rat
• sperm
• timokinon

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