Sıçanlarda Sodyum Valproat ile İndüklenen Dalak Toksisitesinde Rutinin Antioksidan, Antiinflamatuvar ve Antiapoptotik Etkileri

Abstract

Amaç: Antiepileptik bir ilaç olan sodyum valproatın uzun süreli maruziyeti dokularda özellikle oksidatif stres ve inflamasyon artışına neden olarak toksik etki oluşturur. Rutin, birçok bitkide doğal olarak bulunan antioksidan, antiinflamatuvar ve antiapoptotik etkilere sahip bir flavanoiddir. Bu çalışmada, sodyum valproat kaynaklı dalak doku hasarı üzerine doğal bir antioksidan olan rutinin etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: 35 adet erkek sıçan kontrol, sodyum valproat, rutin, sodyum valproat+Rutin 50 ve sodyum valproat+Rutin 100 grupları olmak üzere 5 gruba ayrıldı. 14 gün boyunca 500 mg/kg dozda sodyum valproat uygulamasıyla birlikte 50 veya 100 mg/kg rutin uygulaması oral gavaj yolla yapıldı. 15. günde dalak dokuları alındı ve biyokimyasal yöntemler ile oksidatif stres, inflamasyon ve apoptotik parametrelerin analizi ve histolojik analizler yapıldı. Bulgular: Dalak dokularında sodyum valproat kaynaklı oksidatif stres, inflamasyon ve apoptoz parametrelerin düzeyleri kontrol grubuna göre artmıştır (p<0,05). Rutin uygulamasıyla birlikte sodyum valproata bağlı tüm bu artış miktarlarında azalmalar meydana gelmiştir (p<0,05). Sonuç: Dalak dokularında sodyum valproat maruziyetinin neden olduğu toksik etkiye karşı rutinin potansiyel koruyucu özelliklere sahip olduğu sonucuna varıldı.

Keywords

• Sodyum Valproat
• Rutin
• Oksidatif stres
• İnflamasyon
• Apoptoz
• Dalak Toksisitesi

Antioxidant, Antiinflammatory, and Antiapoptotic Effects of Rutin in Spleen Toxicity Induced by Sodium Valproate in Rats

Abstract

Aim: Long-term exposure to sodium valproate, an antiepileptic drug, causes toxic effects in tissues, especially by increasing oxidative stress and inflammation. Rutin is a flavanoid with antioxidant, anti-inflammatory and antiapoptotic effects found naturally in many plants. In this study, we aimed to investigate the effects of rutin, a natural antioxidant, on sodium valproate-induced spleen tissue damage. Materials and Methods: 35 male rats were divided into 5 groups as control, sodium valproate, rutin, sodium valproate+Rutin 50 and sodium valproate+Rutin 100 groups. For 14 days, 500 mg/kg dose of sodium valproate and 50 or 100 mg/kg of rutin were administered by oral gavage. On day 15, spleen tissues were removed and biochemical methods, oxidative stress, inflammation and apoptotic parameters were analyzed and histologic analysis was performed. Results: The levels of sodium valproate-induced oxidative stress, inflammation and apoptosis parameters increased in spleen tissues compared to the control group (p<0.05). With routine administration, all of these sodium valproate-induced increases were decreased (p<0.05). Conclusion: It was concluded that rutin has potential protective properties against the toxic effect caused by sodium valproate exposure in spleen tissues.

Keywords

• Sodium Valproate
• Rutin
• Oxidative stress
• Inflammation
• Apoptosis
• Spleen Toxicity

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