Protective Effect of Royal Jelly on Some Hematologic Parameters Against Sodium Arsenite Toxicity: A Study in Rats

Abstract

This study examined the effect of royal jelly on some hematological values against sodium arsenite (NaAsO2)-induced toxicity in rats. Royal jelly is a known functional bee product; therefore, its potential to protect against these effects is of great importance. In this study, the effects of royal jelly on hematologic changes were investigated. In the study, 35 healthy Wistar albino male rats weighing 250–300 g and 12–13 weeks old were used. Rats were divided into different groups by gavage daily for 14 days. WBC, LYM, MID, GRA, RBC, HGB, MCHC, MCH, MCV, HCT, PLT, MPV, and PDW values were measured in blood samples. When the measured values were analyzed, the MID, RBC, MCHC, MCH, MCV, and PDV values did not show statistical significance in all experimental groups (P>0.005). However, significant decreases in %LYM, %MID, HCT, and PLT values and increases in WBC, GRA, and %GRA counts were observed in all groups (P < 0.005). This review shows that royal jelly has a potential effect on the hematological and immune systems against toxicity caused by harmful heavy metals such as arsenic. In particular, further research is required to identify the most effective dosage of royal jelly. The results highlight the protective potential of royal jelly against toxicity, which requires further investigation.

Keywords

• Royal jelly
• sodium arsenite
• hematological parameters
• heavy metal toxicity

References

1. Beltekin B, Demir N. International Journal of Food, Agriculture and Animal Sciences ( IJFAA ) Production and Some Bioactive Properties of Royal Jelly- A Review Arı Sütü Üretimi ve Bazı Biyoaktif Özellikleri - Derleme. 2022; 2(1): 38–46.
2. Ramadan MF, Al-Ghamdi A. Bioactive Compounds and Health-Promoting Properties of Royal Jelly: A Review. J. Funct. Foods 2012; 4(1): 39–52.
3. Fratini F, Cilia G, Mancini S, Felicioli A. Royal Jelly: An Ancient Remedy with Remarkable Antibacterial Properties. Microbiol. Res. 2016; 192: 130–141.
4. Özbolat G, Tuli A. Ağır Metal Toksisitesinin İnsan Sağlığına Etkileri. Arşiv Kaynak Tarama Derg. 2016; 25(23783): 502–521.
5. Seven T, Can B, Darende BN, Ocak S. Hava ve Toprakta Ağır Metal Kirliliği. Ulus. Çevre Bilim. Araştırma Derg. 2018; 1(2): 91–103.
6. Başkan MB, Pala A. İçme Sularinda Arsenik Ki̇rli̇li̇ği̇ : Ülkemi̇z Açisindan Bi̇r Değerlendi̇rme. Pamukkale Üniversitesi Mühendislik Bilim. Derg. 2009; 15(1): 69–79.
7. Bolliger AP, Everds N. Haematology of the Mouse. Lab. Mouse 2012: 331–347.
8. Kelada SNP, Aylor DL, Peck BCE, Ryan JF, Tavarez U, Buus RJ, Chesler DRM, Threadgill DW, Churchill GA, de Villena FPM, Collins FS. Genetic Analysis of Hematological Parameters in Incipient Lines of the Collaborative Cross. G3 Genes, Genomes, Genet. 2012; 2(2): 157–165.

9. Lakna. DifferencACe Between Gram Positive and Gram Negative Bacteria Stunning Images of Cells Discover How Scientists Use Main Difference AC– Gram Positive vs Gram Negative Bacteria. Pediaa 2017; (April): 13.
10. Kumar V. R. Robbins Temel Patoloji. İstanbul: Nobel 2003.
11. Ola-Davies OE, Akinrinde AS. Acute Sodium Arsenite-Induced Hematological and Biochemical Changes in Wistar Rats: Protective Effects of Ethanol Extract of Ageratum Conyzoides. Pharmacognosy Res. 2016; 8(Suppl 1): S26.
12. Mank V, Azhar W, Brown K. Leukocytosis. StatPearls 2023.
13. Tigner A, Ibrahim SA, Murray I V. Histology, White Blood Cell. StatPearls 2022.
14. Frandson RD, Wilke WL, Fails AD. Anatomy and Physiology of Farm Animals. 2009: 512.
15. Linke B, Schreiber Y, Picard-Willems B, Slattery P, Nüsing RM, Harder S, Geisslinger G, Scholich K. Activated Platelets Induce an Anti-Inflammatory Response of Monocytes / Macrophages through Cross-Regulation of PGE 2 and Cytokines. 2017; 2017.
16. Ciarrocca M, Tomei G, Palermo P, Caciari T, Cetica C, Fiaschetti M, Gioffrè PA, Tasciotti Z, Tomei F, Sancini A. Environmental and Biological Monitoring of Arsenic in Outdoor Workers Exposed to Urban Air Pollutants. Int. J. Hyg. Environ. Health 2012; 215(6): 555–561.
17. Dacie J V, Lewis SM. Practical Haematology. 1995: 609–609.
18. Kaya E, Şimşek H, Çakar F. Arı Sütünün Senesens ve Telomer Etkisi. In:. Sağlık Bilim. Alanında Uluslararası Araştırmalar IX. 2022; 83–95.
19. Nikolić R, Krstić N, Jovanović J, Kocić G, Cvetković TP, Radosavljević-Stevanović N. Monitoring the Toxic Effects of Pb, Cd and Cu on Hematological Parameters of Wistar Rats and Potential Protective Role of Lipoic Acid and Glutathione. Toxicol. Ind. Health 2015; 31(3): 239–246.
20. Ramadan MF, Al-Ghamdi A. Bioactive Compounds and Health-Promoting Properties of Royal Jelly: A Review. J. Funct. Foods 2012; 4(1): 39–52.
21. Smedley PL, Kinniburgh DG. United Nations Synthesis Report on Arsenic in Drinking Water Developed on Behalf of the United Nations Administrative Committee on Cooperation Sub-Committee on Water Resources, with Active Participation of UNICEF, UNIDO, IAEA, and the World Bank. Br. Geol. Surv. Wallingford 2001:1–390.
22. Ohaeri CC, Eluwa MC. Abnormal Biochemical and Haematological Indices in Trypanosomiasis as a Threat to Herd Production. Vet. Parasitol. 2011; 177(3–4): 199–202.
23. Kumari S, Rani S, Kumar A. Ameliorative Effect of Panax Quinquefolius on Sodium Arsenite Induced Toxicity in Charles Foster Rats. J. Appl. Nat. Sci. 2022; 14(4): 1532–1541.
24. Sharma S, Rani S. Possible Protective Role of Aqueous Tomato Extract on Hemato-Biochemical Parameters against Sodium Arsenite Toxicity in Albino Rats. J. Pharm. Res. Int. 2022; 34(14B): 12–22.
25. Lin A, Loré K. Granulocytes: New Members of the Antigen-Presenting Cell Family. Front. Immunol. 2017; 8(DEC): 1781.
26. Blumenreich MS. The White Blood Cell and Differential Count. Clin. Methods Hist. Phys. Lab. Exam. 1990.
27. Adebayo AH, Zeng GZ, Fan JT, Ji CJ, He WJ, Xu JJ, Zhang YM, Akindahunsi AA, Kela R, Tan NH. Biochemical, Haematological and Histopathological Studies of Extract of Ageratum Conyzoides L. in Sprague Dawley Rats. J. Med. Plants Res. 2010; 4(21): 2264–2272.
28. Ewere EG, Okolie NP, Ndem JI, Oyebadejo SA, Ewere EG, Okolie NP, Ndem JI, Oyebadejo SA. Immunological and Hematological Effects of Irvingia Gabonensis Stem Bark in Sodium Arsenite-Exposed Rats. Https://Gsconlinepress.Com/Journals/Gscbps/Sites/Default/Files/GSCBPS-2021-0089.Pdf 2021; 15(1): 027–037.
29. NHLBI, NIH. Lymphopenia - What Is Lymphopenia? Https//Www.Nhlbi.Nih.Gov/Health/Lymphopenia Date Accessed 2023-09-07 2022.
30. Lu M, Wang H, Li XF, Lu X, Cullen WR, Arnold LL, Cohen SM, Le XC. Evidence of Hemoglobin Binding to Arsenic as a Basis for the Accumulation of Arsenic in Rat Blood. Chem. Res. Toxicol. 2004; 17(12): 1733–1742.
31. Szymańska-Chabowska A, Antonowicz-Juchniewicz J, Andrzejak R. Some Aspects of Arsenic Toxicity and Carcinogenicity in Living Organism with Special Regard to Its Influence on Cardiovascular System, Blood and Bone Marrow. Int. J. Occup. Med. Environ. Health 2002; 15(2): 101–116.
32. Ferland R, Gadahi JA, Saleha S, Ali Q. Histological and Haematological Disturbance Caused by Arsenic Toxicity in Mice Model. Pakistan J. Biol. Sci. 2008; 11(11): 1405–1413.
33. Basher MK, Sarkar S, Mobarak MH, Islam MR. Inorganic Arsenic Exposure during Pregnancy Affects Post-Natal Growth, Blood Parameters, and Organ Development of Mice Offspring. J. Adv. Biotechnol. Exp. Ther. 2023; 6(2): 445–457.
34. Kundrapu S, Noguez J. Laboratory Assessment of Anemia, vol. 83. Elsevier Inc. 2018.
35. Korniluk A, Koper-Lenkiewicz OM, Kamińska J, Kemona H, Dymicka-Piekarska V. Mean Platelet Volume (MPV): New Perspectives for an Old Marker in the Course and Prognosis of Inflammatory Conditions. Mediators Inflamm. 2019; 2019.
36. Sikora J, Kostka B. Blood Platelets as Pharmacological Mode. Postępy Biol. Komórki 2005.
37. Kalia K, Narula GD, Kannan GM, Flora SJS. Effects of Combined Administration of Captopril and DMSA on Arsenite Induced Oxidative Stress and Blood and Tissue Arsenic Concentration in Rats. Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 2007; 144(4): 372–379.
38. Thompson CB, Jakubowski JA. The Pathophysiology and Clinical Relevance of Platelet Heterogeneity. Blood 1988; 72(1): 1–8.