Investigation of the Protective Effects of Chrysin Against Paclitaxel-Induced Oxidative Stress and Apoptosis in Human Neuronal SH-SY5Y Cells

Abstract

In this study, the potential protective effects of chrysin, an important flavonoid, against paclitaxel-induced cell toxicity in the SH-SY5Y nerve cell line as an in vitro model, were investigated by cell viability analysis, lipid peroxidation analysis and quantitative simultaneous PCR methods. In the study, firstly, paclitaxel and chrysin were applied to the SH-SY5Y cell line at different concentrations in the range of 0-30 µM, and the results showed that 15 and 30 µM paclitaxel reduced cell viability, and 500 and 1000 µM chrysin application reduced these effects. In addition, chrysin application has been shown to significantly reduce malondialdehyde levels in paclitaxel-induced cells. The study also examined the effects of paclitaxel and chrysin application on apoptotic and antiapoptotic genes, mostly located in the intrinsic pathway, and showed that chrysin significantly reduced the levels of caspase 10, caspase 8, caspase 6, p53 and NFKB, and increased the Bcl-2 level compared to the paclitaxel-treated group. The results of this study suggest that chrysin's suppression of oxidative stress and apoptotic cell death may be an effective strategy for the treatment of paclitaxel-induced SH-SY5Y cytotoxicity.

Keywords

• Paclitaxel
• chrysin
• apoptosis
• Bcl-2
• malondialdehyde levels

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