The Effect of Sodıum Hypochlorite and Hydrogen Peroxide on the Vase life of Cut Rose Flowers

Abstract

Cut roses are a popular choice for bouquets and floral arrangements, but their short vase life can be a significant drawback. this study investigated the use of hydrogen peroxide (H2O2) and sodium hypochlorite as potential treatments to extend the vase life of cut roses. Cut flowers (R. hybrida cv ‘Samourai’) were placed in glass containing solutions in six different treatments: H2O2, 400, 600, 800 μM; Sodium hypochlorite (NaOCl, 50, 100, 200 mg L-1) and distilled water as a control. Vase life, fresh flower weight, water uptake, color change of flower, flower open ratio, pH and EC variation were among the parameters that were noted based during 15 days According to the results, the solution containing 600 μM of H2O2 produced the highest values for vase life, fresh weight, water uptake, and least color change of the flowers. This was followed by the solution containing 400 μM of H2O2. Sodium hypoclite 50 mg L-1 treatment together with the control during the vase life gave the lowest results both in terms of vase life and water uptake and in terms of preserving the quality of the flowers.The quality and vase life of cut rose flowers were found to be significantly affected by the use of 400 μM and 600 μM H2O2 in preservation solutions.

Keywords

• Cut flowers
• Water uptake
• Hydrogen peroxide
• Sodium hypochlorite
• Cut rose

Sodyum Hı̇poklorı̇t ve Hı̇drojen Peroksı̇dı̇n Kesme Gül Çı̇çeklerı̇nı̇n Vazo Ömrü Üzerı̇ne Etkı̇sı̇

Abstract

Kesme güller buketler ve çiçek aranjmanları kullanılan önemli süs bitkilerindendir ancak kısa vazo ömürleri bu kullanımları için dezavantaj oluşturmaktadır. Bu çalışmada, farklı dozlarda hidrojen peroksit (H2O2) ve sodyum hipoklorit (NaOCl) kullanılarak kesme güllerin vazo ömrüne etkisi araştırılmıştır. Çalışmada R. hybrida cv 'Samourai' kesme çiçekleri 6 faklı uygulama içerisindeki solüsyonlara yerleştirilmiştir. Hidrojen peroksit, 400, 600, 800 μM dozları ile Sodyum hipoklorit 50, 100, 200 mg L-1 dozları kullanılan araştırmada kontrol grubu olarak ise distile su kullanılmıştır. Vazo ömrü, taze çiçek ağırlığı, toplam su alımı, goncaların renk değişimi, çiçek açılım oranı, pH ve EC değişimi 15 gün boyunca izlenmiştir. Sonuçlara göre, 600 μM H2O2 uygulaması vazo ömrü, taze ağırlık, su alımı ve en az çiçek renk değişimi bakımından en yüksek değerlere sahipti. Bunu 400 μM H2O2 içeren uygulama takip etmiştir. Vazo ömrü boyunca kontrol ile birlikte 50 mg L-1 sodyum hipoklorit uygulaması hem vazo ömrü, su alımı bakımından hem de çiçeklerin kalitelerinin korunmasında iyi bir sonuç vermemiştir. 400 μM ve 600 μM H2O2 içeren vazo solüsyonlarının kullanılması kesme gül çiçeklerinin kalitesinin ve vazo ömrünün önemli ölçüde etkilendiği bulunmuştur. Sonuç olarak 400 ve 600 μM H2O2’nin kesme gül çiçeklerinde vazo ömrünü uzattığı ve çiçek kalitesini koruduğu belirlenmiştir.

Keywords

• Kesme çiçek
• Su alımı
• Hidrojen peroksit
• Sodyum hipoklorit
• Kesme gül

References

1. Thakur N. A review on the effect of storage methods and packaging material on the post-harvest longevity of cut flowers. Int J Chem Stud. 2020; 1;8(3):2375–9.
2. Mayak S, Halevy AH, Sagie S, Bar-Yoseph A, Bravdo B. The water balance of cut rose flowers. Physiol Plan. 1974;31:15–22.
3. Ichimura K, Kawabata Y, Kishimoto M, Goto R, Yamada K. Variation with the cultivar in the vase life of cut rose flowers. Bull Natl Inst Flor Sci. 2002;2:9–20.
4. Pizano M. Research shows the way for postharvest treatment roses. Flower Technol. 2009;12(6):1–13.
5. van Doorn WG. Water relations of cut flowers. Hort Rev. 1997;18:1–85.
6. Meral DE. Increasing the vase life of cut carnation (Dianthus caryophyllus L. ‘Baltico’) by reducing xylem congestion with some solutions. ISPEC J Agric Sci. 2024;8(3):698–708.
7. Kazaz S, Doğan E, Kılıç T, Ergür EG, Seyhan S. Influence of Holding Solutions on vase life of cut Hydrangea Flowers (Hydrangea macrophylla Thunb.). Fresenius Environ Bull. 2019;28(4A):3554–2559.
8. Kazaz S, Kılıç T, Doğan E, Sekmen Ş. Vase life extension of cut hydrangea (Hydrangea macrophylla ) flowers. J Hortic Sci Biotechnol. 2020;95(3):325–30.

9. Reid MS, Jiang C. Postharvest Biology and Technology of Cut Flowers and Potted Plants. In: Horticultural Reviews. Wiley; 2012; p. 1–54.
10. Akhtar G, Rajwana IA, Sajjad Y, Shehzad MA, Amin M, Razzaq K, et al. Do natural leaf extracts involve regulation at physiological and biochemical levels to extend vase life of gladiolus cut flowers? Sci Hortic. 2021;282:110042.
11. Li Z, Zhou W, Wang P, Chen Y, Huo S, Wang J, et al. Transcriptome Analysis Reveals the Senescence Process Controlling the Flower Opening and Closure Rhythm in the Waterlilies (Nymphaea L.). Front Plant Sci. 2021; 4;12.
12. van Doorn WG, Schurer K, de Witte Y. Role of Endogenous Bacteria in Vascular Blockage of Cut Rose Flowers. J Plant Physiol. 1989;134(3):375–81.
13. Prochazkova D, Sairam RK, Srivastava GC, Singh DV. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci. 2001;161(4):765–71.
14. Uchida A, Jagendorf AT, Hibino T, Takabe T, Takabe T. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Sci. 2002;163(3):515–23.
15. Neill S. Hydrogen peroxide signalling. Curr Opin Plant Biol. 2002 ;5(5):388–95.
16. Skutnik E, Lukaszewska A, Serek M, Rabiza J. Effect of growth regulators on postharvest characteristics of Zantedeschia aethiopica. Postharvest Biol Technol. 2001;21(2):241–6.
17. Liao W, Zhang ML, Huang GB, Yu J. Hydrogen peroxide in the vase solution increases vase life and keeping quality of cut Oriental×Trumpet hybrid lily “Manissa.” Sci Hortic 2012;139:32–8.
18. Fath A, Bethke PC, Jones RL. Enzymes That Scavenge Reactive Oxygen Species Are Down-Regulated Prior to Gibberellic Acid-Induced Programmed Cell Death in Barley Aleurone. Plant Physiol. 2001;126(1):156–66.
19. Potocký M, Jones MA, Bezvoda R, Smirnoff N, Žárský V. Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth. New Phytol. 2007; 21;174(4):742–51.
20. Liao W, Huang G, Yu J, Zhang M, Shi X. Nitric oxide and hydrogen peroxide are involved in indole-3-butyric acid-induced adventitious root development in marigold. J Hortic Sci Biotechnol. 2011 7;86(2):159–65.
21. Langston BJ, Bai S, Jones ML. Increases in DNA fragmentation and induction of a senescence-specific nuclease are delayed during corolla senescence in ethylene-insensitive (etr1-1) transgenic petunias. J Exp Bot. 2005;56(409):15–23.
22. Singh A, Kumar J, Kumar P. Effects of plant growth regulators and sucrose on post harvest physiology, membrane stability and vase life of cut spikes of gladiolus. Plant Growth Regul. 2008;55(3):221–9.
23. Ferrante A, Vernieri P, Serra G, Tognoni F. Changes in abscisic acid during leaf yellowing of cut stock flowers. Plant Growth Regul. 2004;43(2):127–34.
24. Mutui TM, Emongor VE, Hutchinson M. No The effects of gibberellin 4+7 on the vase life and flower quality of Alstroemeria cut flowers. Plant Growth Regul. 2006;48:207–14.
25. Zhang F, Wang Y, Yang Y, Wu H, Wang D, Liu J. Involvement of hydrogen peroxide and nitric oxide in salt resistance in the calluses from Populus euphratica. Plant Cell Environ. 2007 Jul 23;30(7):775–85.
26. Bright J, Desikan R, Hancock JT, Weir IS, Neill SJ. ABA‐induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant J. 2006 Jan 6;45(1):113–22.
27. Garcı́a-Mata C, Lamattina L. Nitric Oxide Induces Stomatal Closure and Enhances the Adaptive Plant Responses against Drought Stress. Plant Physiol. 2001 Jul 1;126(3):1196–204.
28. Ishibashi Y, Yamaguchi H, Yuasa T, Iwaya-Inoue M, Arima S, Zheng SH. Hydrogen peroxide spraying alleviates drought stress in soybean plants. J Plant Physiol. 2011 Sep;168(13):1562–7.
29. Shanan N. Optimum pH Value for Improving Postharvest Characteristics and Extending Vase Life of Rosa hybrida cv. Tereasa Cut Flowers. Asian J Adv Agric Res. 2017;1(3):1–11.
30. Paul D, Jannat A, Mahmud A, Akhter MJ, Mahmood S. Preservative solutions on vase life and quality of cut Polianthes tuberosa L. Ornam Hortic. 2021 Sep;27(3):417–24.