Abstract

GST izozimlerinin prognostik değerini değerlendirmek için GST izozim düzeyleri ile tümör belirteçleri arasındaki korelasyonları araştırmak. Bu retrospektif çalışmada adenokarsinom ve skuamöz hücreli karsinomu olan 40 hastanın klinik verileri analiz edilmiştir. Tümör ve komşu sağlıklı doku örnekleri, GST enzimlerinin (Sigma, Omega, Pi, Mu) ve kaspaz-3, Bcl-2, p38, p53'ün profilini çıkarmak için immünohistokimyasal olarak boyandı. Protein ekspresyon düzeyleri ile hasta özellikleri arasındaki ilişkiler incelenmiş ve GST enzimleri ile Kaspaz-3, Bcl-2, p38, p53 arasındaki korelasyonlar analiz edilmiştir. Tüm belirteçler için tümörlü ve sağlıklı dokular arasında anlamlı immünohistokimyasal farklılıklar bulunmuştur. GST enzimleri (GSTS, GSTO, GSTP, GSTM) ağırlıklı olarak tümörlü dokularda eksprese edilmiş, GSTO ve GSTP yüksek ekspresyon seviyeleri göstermiştir. SCC dokularıyla karşılaştırıldığında, GSTP ekspresyonu AC dokularında yaklaşık %30 daha yüksektir. Buna karşılık, GSTO ekspresyonu ikinci evre tümörlerde, özellikle de AC dokularında yaklaşık %25 oranında artmaktadır. Korelasyon analizi, tümör dokularında Bcl-2 ile kaspaz-3, p38, GSTS arasında, kaspaz-3 ile GSTP arasında ve p38 ile GSTM arasında anlamlı pozitif ilişkiler olduğunu ortaya koymuştur. Çalışma, KHDAK'de GST izozimlerinin prognostik değerini desteklemektedir.

Keywords

• Lung cancer
• Glutatione-S Transferase
• Caspase-3
• Bcl-2
• p38
• p53

The Potential Prognostic Value of Glutatione-S Transferase Izoenzymes in Non-small Cell Lung Cancer

Abstract

GST izozimlerinin prognostik değerini değerlendirmek için GST izozim düzeyleri ile tümör belirteçleri arasındaki korelasyonları araştırmak. Bu retrospektif çalışmada adenokarsinom ve skuamöz hücreli karsinomu olan 40 hastanın klinik verileri analiz edilmiştir. Tümör ve komşu sağlıklı doku örnekleri, GST enzimlerinin (Sigma, Omega, Pi, Mu) ve kaspaz-3, Bcl-2, p38, p53'ün profilini çıkarmak için immünohistokimyasal olarak boyandı. Protein ekspresyon düzeyleri ile hasta özellikleri arasındaki ilişkiler incelenmiş ve GST enzimleri ile Kaspaz-3, Bcl-2, p38, p53 arasındaki korelasyonlar analiz edilmiştir. Tüm belirteçler için tümörlü ve sağlıklı dokular arasında anlamlı immünohistokimyasal farklılıklar bulunmuştur. GST enzimleri (GSTS, GSTO, GSTP, GSTM) ağırlıklı olarak tümörlü dokularda eksprese edilmiş, GSTO ve GSTP yüksek ekspresyon seviyeleri göstermiştir. SCC dokularıyla karşılaştırıldığında, GSTP ekspresyonu AC dokularında yaklaşık %30 daha yüksektir. Buna karşılık, GSTO ekspresyonu ikinci evre tümörlerde, özellikle de AC dokularında yaklaşık %25 oranında artmaktadır. Korelasyon analizi, tümör dokularında Bcl-2 ile kaspaz-3, p38, GSTS arasında, kaspaz-3 ile GSTP arasında ve p38 ile GSTM arasında anlamlı pozitif ilişkiler olduğunu ortaya koymuştur. Çalışma, KHDAK'de GST izozimlerinin prognostik değerini desteklemektedir.

Keywords

• Lung cancer
• Glutatione-S Transferase
• Caspase-3
• Bcl-2
• p38
• p53

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