Production of Cellulase Enzyme by Pseudomonas reinekei SL7 Isolated from Paper Mill Wastes

Abstract

The constantly growing energy requirements of today’s society and swiftly depleting fossil fuels indicate a need for a renewable alternatives. Cellulose is regarded as an alternative energy source and has recently become significant around the globe. Its obstinate structure must be degraded to utilize this valuable resource. Cellulose-degrading microorganisms are of great importance in the efficient use of cellulose resources. Screening of natural cellulolytic microorganisms and optimization of fermentation conditions are the focal points of research studies. Waste from paper mills is considered to be one of the main sources of cellulolytic bacteria. In the current study, isolation of cellulolytic bacteria was performed from paper mill wastes. After the determining the isolate showing the best cellulase activity, it was identified by sequencing the 16S rRNA gene region. According to the sequence analysis, it was detected that the isolate SL7 showed similarity to Pseudomonas reinekei with the rate of 99%. By optimizing the process parameters, the highest production of cellulase was obtained at temperature of 30 ⁰C, pH 7, carboxymethyl cellulose (CMC) concentration of 20 g/L, yeast extract concentration of 2 g/L and incubation time of 60 h. Under optimized conditions, the enzyme activity was detected to be 34.2 U/mL.

Keywords

• Cellulase
• Pseudomonas reinekei SL7
• Paper Mill Wastes

Kağıt Fabrikası Atıklarından İzole Edilen Pseudomonas reinekei SL7 Suşu ile Selülaz Enzimi Üretimi

Abstract

Günümüz toplumunun sürekli artan enerji gereksinimleri ve hızla tükenen fosil yakıtlar, yenilenebilir alternatiflere olan ihtiyacı göstermektedir. Selüloz alternatif bir enerji kaynağı olarak kabul edilmekte ve son zamanlarda dünya çapında önemli hale gelmiştir. Bu değerli kaynağın kullanılabilmesi için inatçı yapısının parçalanması gerekmektedir. Selüloz parçalayan mikroorganizmalar, selüloz kaynaklarının verimli kullanımında büyük öneme sahiptir. Doğal selülolitik mikroorganizmaların taranması ve fermantasyon koşullarının optimizasyonu araştırma çalışmalarının odak noktalarıdır. Kağıt fabrikası atıkları, selülolitik bakterilerin başlıca kaynaklarından biri olarak düşünülmektedir. Mevcut çalışmada, kağıt fabrikası atıklarından selülolitik bakteri izolasyonu gerçekleştirilmiştir. En iyi selülaz aktivitesini gösteren izolat belirlendikten sonra, 16S rRNA gen bölgesinin sekans analizi ile tanımlanmıştır. Dizi analizine göre, SL7 kodlu izolatın Pseudomonas reinekei'ye %99 oranında benzerlik gösterdiği tespit edilmiştir. Proses parametrelerinin optimize edilmesiyle en yüksek selülaz üretimi 30 ⁰C sıcaklık, pH 7, 20 g/L karboksimetil selüloz (CMC) konsantrasyonu, 2 g/L maya ekstraktı konsantrasyonu ve 60 saatlik inkübasyon süresinde elde edilmiştir. Optimize edilmiş koşullar altında enzim aktivitesinin 34,2 U/mL olduğu tespit edilmiştir.

Keywords

• Selülaz
• Pseudomonas reinekei SL7
• Kağıt Fabrikası Atıkları

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