Anti-Alzheimer's and Antioxidant Effects of Anacardium Occidentale Oil: Profile Analysis Using GC/MS and HPLC

Abstract

Determining the biological profiles of oils is important for a healthy life and the treatment of common global diseases. In this study, the anti-Alzheimer's and antioxidant properties of A. occidentale oil were investigated using various methods. The inhibitory effects of A. occidentale oil on acetylcholinesterase and butyrylcholinesterase, which are linked to neurodegenerative diseases, were also determined. Phenolic compounds in A. occidentale oil were determined by HPLC chromatography, and thirteen different phenolic compounds were identified. The chemical components of A. occidentale oil were evaluated by GC/MS analysis, and the predominant essential oils were oleic acid (41.25%), linoleic acid (37.95%), and palmitic acid (15.03%). The radical scavenging ability of A. occidentale oil was investigated using ABTS and DPPH bioanalytical radical scavenging methods. The results revealed strong radical scavenging properties with IC50 of 4.33 μg/mL for ABTS radicals and 14.74 μg/mL for DPPH radicals. The research was further extended by examining the reducing abilities of Cuprac and FRAP. A. occidentale oil showed inhibitory effects on acetylcholinesterase (IC50: 13.86 μg/mL) and butyrylcholinesterase (IC50: 16.11 μg/mL). The study will form the basis for future research on the antioxidant properties and enzyme inhibition of oils obtained from different sources of medical and industrial importance.

Keywords

• Anacardium occidentale
• Chromatography
• Antioxidant Activity
• Enzyme Inhibition

Anacardium Occidentale Yağının Alzheimer Karşıtı ve Antioksidan Etkileri: GC/MS ve HPLC Kullanılarak Profil Analizi

Abstract

Yağların biyolojik profillerinin belirlenmesi, sağlıklı bir yaşam ve yaygın küresel hastalıkların tedavisi için önemlidir. Bu çalışmada, A. occidentale yağının Alzheimer karşıtı ve antioksidan özellikleri çeşitli yöntemler kullanılarak araştırılmıştır. A. occidentale yağının nörodejeneratif hastalıklarla bağlantılı olan asetilkolinesteraz ve bütirilkolinesteraz üzerindeki inhibitör etkileri de belirlenmiştir. A. occidentale yağındaki fenolik bileşikler HPLC kromatografisi ile belirlenmiş ve on üç farklı fenolik bileşik tanımlanmıştır. A. occidentale yağının kimyasal bileşenleri GC/MS analizi ile değerlendirilmiş ve baskın uçucu yağlar oleik asit (%41.25), linoleik asit (%37.95) ve palmitik asit (%15.03) olmuştur. A. occidentale yağının radikal süpürücü özelliği, ABTS ve DPPH biyoanalitik radikal süpürücü yöntemleri kullanılarak araştırılmıştır. Sonuçlar, ABTS radikalleri için 4.33 μg/mL ve DPPH radikalleri için 14.74 μg/mL IC50 ile güçlü radikal temizleme özellikleri ortaya koydu. Araştırma, Cuprac ve FRAP'ın indirgeyici yeteneklerinin incelenmesiyle daha da genişletildi. A. occidentale yağı, asetilkolinesteraz (IC50: 13.86 μg/mL) ve bütirilkolinesteraz (IC50: 16.11 μg/mL) üzerinde inhibitör etkiler gösterdi. Çalışma, tıbbi ve endüstriyel öneme sahip farklı kaynaklardan elde edilen yağların antioksidan özellikleri ve enzim inhibisyonu üzerine gelecekteki araştırmalar için temel oluşturacaktır.

Keywords

• Anacardium occidentale
• Kromatografi
• Antioksidan Aktivite
• Enzim İnhibisyonu

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