THE EFFECTS OF ROYAL JELLY ON SELECTED SYSTEMIC PARAMETERS AGAINST EXPERIMENTAL LOWER EXREMITY ISCHAEMIA-REPERFUSION INJURY

Abstract

Lower extremity ischemia-reperfusion (I/R) injury is a significant clinical problem that may lead to systemic biochemical alterations and remote organ dysfunction in addition to local tissue damage following surgical and traumatic events. In the present study, the effects of royal jelly (RJ) administration on some systemic parameters were evaluated in rats subjected to an experimental femoral artery I/R model. Male Wistar albino rats were randomly assigned into three groups (n=6): control, I/R and RJ groups. The I/R model was established by femoral artery ligation for 90 minutes followed by 90 minutes of referfusion. Royal jelly was administered orally at a dose of 100 mg/kg for 7 days prior to the surgical procedure. At the end of the reperfusion period, serum levels of LDH, AST, ALT, Na, K, Cl, urea and creatinine were analyzed. Serum LDH, AST, ALT, Na, K, Cl and urea levels were significantly increased in the I/R group compared with the control group (p<0.05). In the RJ-treated group, these parameters were significantly reduced compared with the I/R group and mostly approached control values (p<0.05). No significant difference was observed among the groups in term of creatinine levels (p>0.05). In conclusion, royal jelly administration attenuated systemic biochemical alterations associated with experimental lower extremity I/R injury and exerted a regulatory effect on electrolyte imbalance, suggesting its potential as a supportive agent.

Keywords

• ischemia-reperfusion
• royal jelly
• femoral artery
• serum
• I/R

DENEYSEL ALT EKSTREMİTE İSKEMİ-REPERFÜZYON HASARINA KARŞI ARI SÜTÜNÜN BAZI SİSTEMİK PARAMETRELER ÜZERİNE ETKİSİ

Abstract

Alt extremite iskemi-reperfüzyon (I/R) hasarı, cerrahi girişimler ve travmatik süreçler sonrasında lokal doku hasarının yanı sıra sistemik biyokimyasal değişikliklere ve uzak organ disfonksiyonuna yol açabilen önemli bir klinik sorundur. Sunulan çalışmanın amacı, deneysel femoral arter I/R modeli oluşturulan sıçanlarda arı sütü (RJ) uygulamasının bazı sistemik parametreler üzerindeki etkilerini değerlendirmektir. Çalışmada Wistar albino ırkı erkek sıçanlar rastgele üç gruba ayrıldı (n=6): kontrol grubu, I/R grubu ve RJ grubu. I/R modeli, femoral arterin 90 dakika süreyle ligasyonu ve ardından 90 dakika reperfüzyon uygulamasıyla oluşturuldu. RJ grubuna, cerrahi işlemden önce 7 gün süreyle oral yolla 100 mg/kg dozunda arı sütü verildi. Reperfüzyon süresi sonunda alınan kan örneklerinde serum LDH, AST, ALT, Na, K, Cl, üre ve kreatinin düzeyleri analiz edildi. Sonuçlar, I/R grubunda serum LDH, AST, ALT, Na, K, Cl ve üre düzeylerinin kontrol grubuna kıyasla istatistiksel olarak anlamlı şekilde arttığını gösterdi (p<0.05). RJ uygulanan grupta ise bu parametrelerin I/R grubuna göre anlamlı biçimde azaldığı ve çoğunun kontrol grubuna yaklaştığı belirlendi (p<0.05). Kreatinin düzeyleri açısından gruplar arasında anlamlı bir fark saptanmadı (p>0.05). Sonuç olarak RJ uygulamasının deneysel alt extremite I/R hasarına bağlı gelişen sistemik biyokimyasal değişiklikleri azalttığı ve elektrolit dengesini düzenleyici etki gösterdiği sonucuna varıldı.

Keywords

• iskemi-reperfüzyon
• arı sütü
• femoral arter
• serum
• I/R

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