The apoptotıc effects of SCFAs from Lactobacillus reuteri on (HT-29) human colon cancer cells

Abstract

Colon cancer is one of the leading causes of death around the World. In colon or other types of cancer, chemical methods can be considered as a therapeutic strategy. However, probiotics can also be used as biotherapeutics to reduce recurrence and side effects in patients with colon cancer.

Short Chain Fatty Acids (SCFAs) the fermentation products of probiotic L. reuteri bacteria found in the gastrointestinal tract. There are several SCFAs including acetic, propionic, butyric and lactic acids that have been shown to have apoptosis inducing properties in human colon cancer cells. In this study, the anti-proliferative and pro-apoptotic activities of probiotics were explained and the effects of SCFAs produced by L. reuteri on mitochondrial apoptosis were observed with changes in ROS and LPO production levels on human colon cancer cells (HT-29).

Results suggested that SCFAs from L. reuteri increased the mortality rates of HT-29 cells, increased ROS and LPO production. In addition to these effects, downregulation of Bcl-2, elevation of cytochrome c and overproduction of caspase-3 protein in SCFAs-treated HT-29 cells were observed. It was concluded that SCFAs from L. reuteri have cytotoxic effects in HT-29 colon cancer cells.

Keywords

• Colon cancer,Probiotics,L. reuteri,HT-29,SCFAs,ROS,LPO

Lactobacillus reuteri’den elde edilen KZYA’nin insan kolon kanser hücrelerinde (HT-29) apoptotik etkileri

Abstract

Kolon kanseri, dünyadaki önde gelen ölüm nedenlerinden biridir. Kolon veya diğer kanser tiplerinde, kimyasal yöntemler terapötik bir strateji olarak düşünülebilir. Bununla birlikte, probiyotikler, kolon kanserli hastalarda hastalığın tekrarı ve yan etkilerini azaltmak için biyoterapötikler olarak kullanılabilir.

Kısa Zincirli Yağ Asitleri (KZYA), bağırsakta bulunan probiyotik L. reuteri bakterilerinin fermentasyon ürünleridir. İnsan kolon kanseri hücrelerinde apoptoz indükleyici özelliklere sahip olduğu gösterilen asetik, propiyonik, bütirik ve laktik asitleri içeren birçok KZYA vardır. Bu çalışmada, probiyotiklerin anti-proliferatif ve pro-apoptotik aktiviteleri açıklanmış ve L. reuteri'nin ürettiği KZYA'nin, insan kolon kanseri hücrelerinde (HT-29) mitokondriyal apoptoz yolağına etkileri ve ROS ile LPO üretim düzeylerindeki değişimleri gözlemlenmiştir.

Sonuçlara göre, L. reuteri'den elde edilen KZYA, HT-29 hücrelerinde mortalite oranını, ROS ve LPO üretimini anlamlı şekilde artırmıştır. Bu etkilere ilaveten KZYA ile tedavi edilen HT-29 hücrelerinde Bcl-2 seviyesinin azalması, sitokromun c ve kaspaz-3 miktarlarının yükselmesi gözlenmiştir. Tüm sonuçlar ışığında L. reuteri kaynaklı KZYA'nin kolon kanseri hücrelerinde sitotoksik etkileri olduğu sonucuna varılmıştır.

Keywords

• Kolon kanseri,Probiyotikler,L. reuteri,HT-29,KZYA,ROS,LPO

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