Ratlarda Kadmiyum Kaynaklı Mide Toksisitesine Karşı Karvakrol’un Etkileri

Abstract

Sunulan çalışmanın amacı; ağır metallerden olan kadmiyumun (Cd) neden olduğu mide toksisitesine karşı karvakrol (Krv)’nin etkilerinin araştırılmasıdır. Çalışmada Spraque Dawley cinsi 35 adet erkek rat rastgele 5 gruba ayrıldı: Kontrol grubu, Cd uygulanan grup, Krv 50 mg kg-1 uygulanan grup, Cd + Krv 25 mg kg-1 uygulanan grup ve Cd + Krv 50 mg kg-1 uygulanan grup. Cd’nin glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) gibi antioksidan enzim aktivitelerini ve glutatyon (GSH) düzeylerini azaltıp, lipid peroksidasyonunu (LPO) artırarak oksidatif hasara neden olduğu belirlendi. Ayrıca Cd ile kombine uygulanan Krv uygulamasının GSH seviyesini ve antioksidan enzim aktivitelerini arttırdığı, lipid peroksidasyonunu ise azalttığı tespit edildi. Çalışmada incelenen glikoz düzenleyici protein 78 (GRP78) mRNA transkipt seviyesinin Cd grubunda önemli şekilde arttığı, Krv uygulamasının ise bu gene ait ekspresyon seviyesini azalttığı belirlendi. Mide dokusunda Cd toksikasyonu aktive edici transkripsiyon faktör 6 (ATF6) ekspresyonunu arttırdığı, Krv uygulaması ile bu gene ait ekspresyonda düşüş meydana geldiği saptandı.
Sonuç olarak; Cd kaynaklı mide toksisitesinde Krv’nin yararlı etkilerinin olduğu oksidan-antioksidan denge ile GRP78 ve ATF6 ekspresyonları incelenerek tespit edildi.

Keywords

• Kadmiyum,Karvakrol,Mide,Oksidatif stres

Effects of Carvacrol Against Cadmium-Induced Stomach Toxicity in Rats

Abstract

The aim of this study is investigation of the effects of carvacrol (Crv) against stomach toxicity caused by cadmium (Cd), which is a heavy metal. In the study, 35 male rats of Spraque Dawley were randomly divided into 5 groups: the control group, the group that received Cd, the group recevied Crv 50 mg kg-1, the group that received Cd + Crv 25 mg kg-1, and the group that received Cd + Crv 50 mg kg-1. It has been determined that Cd causes oxidative damage by decreasing antioxidant enzyme activities such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and increasing lipid peroxidation (LPO). In addition, it was found that Crv administration combined with Cd increased GSH level and antioxidant enzyme activities and decreased lipid peroxidation. It was determined that the level of glucose regulated protein 78 (GRP78) mRNA transcript examined in the study increased significantly in the Cd group, while Crv application decreased the expression level of this gene. It was determined that Cd toxicity in the stomach tissue increased activating transcription factor 6 (ATF6) expression and that the expression of this gene decreased with the recevied of Crv.
As a result, it has been determined by examining the oxidant-antioxidant balance with GRP78 and ATF6 expressions that Crv has beneficial effects on Cd-induced stomach toxicity.

Keywords

• Cadmium,Carvacrol,Stomach,Oxidative stress

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