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Alchemilla persica'nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi

Year 2020, , 157 - 169, 30.12.2020
https://doi.org/10.46810/tdfd.823003

Abstract

Alchemilla persica, hekzan, diklorometan, etil asetat, metanol ve su gibi artan polariteye sahip çözücülerde farklı polaritelere sahip bitki özleri elde edildi. Antioksidan aktivitenin belirlenmesinde toplam fenol içeriği, toplam flavonoid içeriği ve likopen içeriği etil asetat, fenolik asit içeriği hegzan ekstraktında; β –karoten içeriği metanol ekstraktında diğer ekstraktlara göre yüksek olarak belirlenmiştir. Toplam antioksidan aktivite, DPPH serbest radikal giderme aktivitesi, metal şelatlama kapasitesi, toplam indirgeme gücü, hidrojen peroksit giderme, hidroksil radikal uzaklaştırma aktiviteleri farklı konsantrasyonlarda belirlenmiştir. Antimikrobiyal aktivitenin belirlenmesinde agar disk difüzyon tekniği kullanıldı. Antimikrobiyal testlerde, Alchemilla persica'nın diklorometan ekstraktı, en yüksek aktiviteye sahip ekstrakt olarak belirlendi.. Etil asetat ekstresinin antimikrobiyal özelliği, diklorometan ekstraktına hemen hemen benzerdi. Bu ekstraktları antimikrobiyal değerlendirmede metanol ve su ekstreleri izledi. Alchemilla persica'nın farklı özütleriyle yapılan antioksidan çalışmaları sonucunda, α-tokoferol, BHA, BHT ve Trolox gibi standart antioksidanlardan daha etkili olduğu bulunmuştur. Bitki ekstraktlarından su ve metanol ekstraktları anti-sitotoksik etki için çalışılmış ve bu ekstraktlar farklı konsantrasyonlarda hücre büyümesini engellemiştir. Çalışmada elde edilen sonuçlar bu Alchemilla persica türüne ait ekstrelerin antioksidan, antimikrobiyal ve antisitotoksik etki gösterdiği belirlenmiştir.
Anahtar Kelimeler: Alchemilla persica., Antisitotoksik Aktivite, Antioksidan Aktivite, Antimikrobiyal Aktivite, Bitki Ekstraktları.

References

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  • [2] Chen CC, Liu LK, Hsu JD, Huang HP, Yang MY, Wang CJ. Mulberry extract inhibits the development of atherosclerosis in cholesterol-fed rabbits. Food Chemistry. 2005;91(4):601-7.
  • [3] Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000;52(4):673-751.
  • [4] Prior RL. Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr. 2003;78(3):570s-8s.
  • [5] Saleem A, Husheem M, Harkonen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula Retz. fruit. Journal of Ethnopharmacology. 2002;81(3):327-36.
  • [6] Zhang YJ, Vareed SK, Nair MG. Human tumor cell growth inhibition by nontoxic anthocyanidins, the pigments in fruits and vegetables. Life Sciences. 2005;76(13):1465-72.
  • [7] Cieslik E, Greda A, Adamus W. Contents of polyphenols in fruit and vegetables. Food Chemistry. 2006;94(1):135-42.
  • [8] Qian JY, Liu D, Huang AG. The efficiency of flavonoids in polar extracts of Lycium chinense Mill fruits as free radical scavenger. Food Chemistry. 2004;87(2):283-8.
  • [9] Sass-Kiss A, Kiss J, Milotay P, Kerek MM, Toth-Markus M. Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International. 2005;38(8-9):1023-9.
  • [10] Trappey A, Bawadi HA, Bansode RR, Losso JN. Anthocyanin profile of mayhaw (Cretaegus opaca). Food Chemistry. 2005;91(4):665-71.
  • [11] Merken HM, Beecher GR. Measurement of food flavonoids by high-performance liquid chromatography: A review. J Agr Food Chem. 2000;48(3):577-99.
  • [12] Demirel Z. Eğirdir Gölünden İzole Edilen Yeşil Mikroalg (Chlorophyta) Scenedesmus Protuberans Fris.’İn Antimikrobiyal Ve Antioksidan Özelliğinin Araştırılması: Ege Üniversitesi; 2006.
  • [13] Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry. 2001;73(2):239-44.
  • [14] Jiangning G, Xinchu W, Hou W, Qinghua L, Kaishun B. Antioxidants from a Chinese medicinal herb – Psoralea corylifolia L. Food Chemistry. 2005;91(2):287-92.
  • [15] Suja KP, Jayalekshmy A, Arumughan C. Antioxidant activity of sesame cake extract. Food Chemistry. 2005;91(2):213-9.
  • [16] Sokmen A, Sokmen M, Daferera D, Polissiou M, Candan F, Unlu M, et al. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteini Afan. (Asteraceae). Phytother Res. 2004;18(6):451-6.
  • [17] Akalın E. Klinik Uygulamada Antibiyotikler ve Diger Antimikrobiyal ilaçlar. : Güneş Kitapevi; 1994. [18] Austin DJ, Kristinsson KG, Anderson RM. The relationship between the volume of antimicrobial consumption in human communities and the frequency of resistance. P Natl Acad Sci USA. 1999;96(3):1152-6.
  • [19] Guillemot D. Antibiotic use in humans and bacterial resistance. Curr Opin Microbiol. 1999;2(5):494-8.
  • [20] Ali-Shtayeh MS, Yaghmour RMR, Faidi YR, Salem K, Al-Nuri MA. Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area. Journal of Ethnopharmacology. 1998;60(3):265-71.
  • [21] Pacheco P, Schmeda-Hirschmann G, Sierra J, Potter CW, Jones BM, Moshref M. Antiviral activity of chilean medicinal plant extracts. Phytotherapy Research 7(6): 415 - 418. 1993;7(6):415-8.
  • [22] Loy G, Cottiglia F, Garau D, Deidda D, Pompei R, Bonsignore L. Chemical composition and cytotoxic and antimicrobial activity of Calycotome villosa (Poiret) Link leaves. Farmaco. 2001;56(5-7):433-6.
  • [23] Zhao M, Ito Y, Tu P. Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography. J Chromatogr A. 2005;1090(1-2):193-6.
  • [24] Gamez-Meza N, Noriega-Rodriguez JA, Medina-Juarez LA, Ortega-Garcia J, Cazarez-Casanova R, Angulo-Guerrero O. Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. J Am Oil Chem Soc. 1999;76(12):1445-7.
  • [25] Barros L, Calhelha RC, Vaz JA, Ferreira ICFR, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology. 2007;225(2):151-6.
  • [26] Koncic MZ, Kremer D, Gruz J, Strnad M, Bisevac G, Kosalec I, et al. Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions. Food Chem Toxicol. 2010;48(6):1537-42.
  • [27] Hatano T, Kagawa H, Yasuhara T, Okuda T. 2 New Flavonoids and Other Constituents in Licorice Root - Their Relative Astringency and Radical Scavenging Effects. Chem Pharm Bull. 1988;36(6):2090-7.
  • [28] Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan Journal of Nutrition. 1986;44:307-15.
  • [29] Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10(6):1003-8.
  • [30] Ozyurek M, Bektasoglu B, Guclu K, Apak R. Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. Anal Chim Acta. 2008;616(2):196-206.
  • [31] Dinis TCP, Madeira, V. M. C. ve Almeidam, L. M., . Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and peroxyl radicals scavengers. Archives of Biochemistry and Biophysic. 1994;315:161-9.
  • [32] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337-41.
  • [33] Berghe VA VA, . Screening methods for antibacterial and antiviral agents from higher plants. Meth Plant Biochem. 1991.;6:47-68.
  • [34] Frankel EN, Meyer AS. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agr. 2000;80(13):1925-41.
  • [35] Albayrak S, Aksoy A, Sagdic O, Budak U. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turk J Biol. 2010;34(4):463-73.
  • [36] Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-78.
  • [37] Perez-Jimenez J, Saura-Calixto F. Grape products and cardiovascular disease risk factors. Nutr Res Rev. 2008;21(2):158-73.
  • [38] Huang ZL, Wang BW, Eaves DH, Shikany JM, Pace RD. Total phenolics and antioxidant capacity of indigenous vegetables in the southeast United States: Alabama Collaboration for Cardiovascular Equality Project. Int J Food Sci Nutr. 2009;60(2):100-8.
  • [39] Tsai TH, Tsai TH, Chien YC, Lee CW, Tsai PJ. In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs. Food Chemistry. 2008;110(4):859-64.
  • [40] Hossain MA, Rahman SMM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Research International. 2011;44(3):672-6.
  • [41] Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004;74(17):2157-84.
  • [42] Eruçar S. Bazı bitkisel çayların fenolik madde profili ve Antioksidan aktivitelerin incelenmesi: İstanbul Teknik Üniversitesi; Fen Bilimleri Enstitüsü. 2006.
Year 2020, , 157 - 169, 30.12.2020
https://doi.org/10.46810/tdfd.823003

Abstract

References

  • [1] Kaya B, Menemen Y, Saltan FZ. Flavonoid compounds identified in Alchemilla L. species collected in the North-eastern Black sea region of Turkey. African Journal of Traditional, Complementary and Alternative Medicines. 2012;9(3).
  • [2] Chen CC, Liu LK, Hsu JD, Huang HP, Yang MY, Wang CJ. Mulberry extract inhibits the development of atherosclerosis in cholesterol-fed rabbits. Food Chemistry. 2005;91(4):601-7.
  • [3] Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000;52(4):673-751.
  • [4] Prior RL. Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr. 2003;78(3):570s-8s.
  • [5] Saleem A, Husheem M, Harkonen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula Retz. fruit. Journal of Ethnopharmacology. 2002;81(3):327-36.
  • [6] Zhang YJ, Vareed SK, Nair MG. Human tumor cell growth inhibition by nontoxic anthocyanidins, the pigments in fruits and vegetables. Life Sciences. 2005;76(13):1465-72.
  • [7] Cieslik E, Greda A, Adamus W. Contents of polyphenols in fruit and vegetables. Food Chemistry. 2006;94(1):135-42.
  • [8] Qian JY, Liu D, Huang AG. The efficiency of flavonoids in polar extracts of Lycium chinense Mill fruits as free radical scavenger. Food Chemistry. 2004;87(2):283-8.
  • [9] Sass-Kiss A, Kiss J, Milotay P, Kerek MM, Toth-Markus M. Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International. 2005;38(8-9):1023-9.
  • [10] Trappey A, Bawadi HA, Bansode RR, Losso JN. Anthocyanin profile of mayhaw (Cretaegus opaca). Food Chemistry. 2005;91(4):665-71.
  • [11] Merken HM, Beecher GR. Measurement of food flavonoids by high-performance liquid chromatography: A review. J Agr Food Chem. 2000;48(3):577-99.
  • [12] Demirel Z. Eğirdir Gölünden İzole Edilen Yeşil Mikroalg (Chlorophyta) Scenedesmus Protuberans Fris.’İn Antimikrobiyal Ve Antioksidan Özelliğinin Araştırılması: Ege Üniversitesi; 2006.
  • [13] Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry. 2001;73(2):239-44.
  • [14] Jiangning G, Xinchu W, Hou W, Qinghua L, Kaishun B. Antioxidants from a Chinese medicinal herb – Psoralea corylifolia L. Food Chemistry. 2005;91(2):287-92.
  • [15] Suja KP, Jayalekshmy A, Arumughan C. Antioxidant activity of sesame cake extract. Food Chemistry. 2005;91(2):213-9.
  • [16] Sokmen A, Sokmen M, Daferera D, Polissiou M, Candan F, Unlu M, et al. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteini Afan. (Asteraceae). Phytother Res. 2004;18(6):451-6.
  • [17] Akalın E. Klinik Uygulamada Antibiyotikler ve Diger Antimikrobiyal ilaçlar. : Güneş Kitapevi; 1994. [18] Austin DJ, Kristinsson KG, Anderson RM. The relationship between the volume of antimicrobial consumption in human communities and the frequency of resistance. P Natl Acad Sci USA. 1999;96(3):1152-6.
  • [19] Guillemot D. Antibiotic use in humans and bacterial resistance. Curr Opin Microbiol. 1999;2(5):494-8.
  • [20] Ali-Shtayeh MS, Yaghmour RMR, Faidi YR, Salem K, Al-Nuri MA. Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area. Journal of Ethnopharmacology. 1998;60(3):265-71.
  • [21] Pacheco P, Schmeda-Hirschmann G, Sierra J, Potter CW, Jones BM, Moshref M. Antiviral activity of chilean medicinal plant extracts. Phytotherapy Research 7(6): 415 - 418. 1993;7(6):415-8.
  • [22] Loy G, Cottiglia F, Garau D, Deidda D, Pompei R, Bonsignore L. Chemical composition and cytotoxic and antimicrobial activity of Calycotome villosa (Poiret) Link leaves. Farmaco. 2001;56(5-7):433-6.
  • [23] Zhao M, Ito Y, Tu P. Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography. J Chromatogr A. 2005;1090(1-2):193-6.
  • [24] Gamez-Meza N, Noriega-Rodriguez JA, Medina-Juarez LA, Ortega-Garcia J, Cazarez-Casanova R, Angulo-Guerrero O. Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. J Am Oil Chem Soc. 1999;76(12):1445-7.
  • [25] Barros L, Calhelha RC, Vaz JA, Ferreira ICFR, Baptista P, Estevinho LM. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology. 2007;225(2):151-6.
  • [26] Koncic MZ, Kremer D, Gruz J, Strnad M, Bisevac G, Kosalec I, et al. Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions. Food Chem Toxicol. 2010;48(6):1537-42.
  • [27] Hatano T, Kagawa H, Yasuhara T, Okuda T. 2 New Flavonoids and Other Constituents in Licorice Root - Their Relative Astringency and Radical Scavenging Effects. Chem Pharm Bull. 1988;36(6):2090-7.
  • [28] Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan Journal of Nutrition. 1986;44:307-15.
  • [29] Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10(6):1003-8.
  • [30] Ozyurek M, Bektasoglu B, Guclu K, Apak R. Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. Anal Chim Acta. 2008;616(2):196-206.
  • [31] Dinis TCP, Madeira, V. M. C. ve Almeidam, L. M., . Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and peroxyl radicals scavengers. Archives of Biochemistry and Biophysic. 1994;315:161-9.
  • [32] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337-41.
  • [33] Berghe VA VA, . Screening methods for antibacterial and antiviral agents from higher plants. Meth Plant Biochem. 1991.;6:47-68.
  • [34] Frankel EN, Meyer AS. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agr. 2000;80(13):1925-41.
  • [35] Albayrak S, Aksoy A, Sagdic O, Budak U. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turk J Biol. 2010;34(4):463-73.
  • [36] Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-78.
  • [37] Perez-Jimenez J, Saura-Calixto F. Grape products and cardiovascular disease risk factors. Nutr Res Rev. 2008;21(2):158-73.
  • [38] Huang ZL, Wang BW, Eaves DH, Shikany JM, Pace RD. Total phenolics and antioxidant capacity of indigenous vegetables in the southeast United States: Alabama Collaboration for Cardiovascular Equality Project. Int J Food Sci Nutr. 2009;60(2):100-8.
  • [39] Tsai TH, Tsai TH, Chien YC, Lee CW, Tsai PJ. In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs. Food Chemistry. 2008;110(4):859-64.
  • [40] Hossain MA, Rahman SMM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Research International. 2011;44(3):672-6.
  • [41] Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004;74(17):2157-84.
  • [42] Eruçar S. Bazı bitkisel çayların fenolik madde profili ve Antioksidan aktivitelerin incelenmesi: İstanbul Teknik Üniversitesi; Fen Bilimleri Enstitüsü. 2006.
There are 41 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Bülent Kaya 0000-0002-1216-6441

Publication Date December 30, 2020
Published in Issue Year 2020

Cite

APA Kaya, B. (2020). Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi. Türk Doğa Ve Fen Dergisi, 9(2), 157-169. https://doi.org/10.46810/tdfd.823003
AMA Kaya B. Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi. TDFD. December 2020;9(2):157-169. doi:10.46810/tdfd.823003
Chicago Kaya, Bülent. “Alchemilla persica’nın Farklı Polaritedeki çözücülerde Antioksidan, Antimikrobial Ve Antiisitotoksik Etkilerinin Belirlenmesi”. Türk Doğa Ve Fen Dergisi 9, no. 2 (December 2020): 157-69. https://doi.org/10.46810/tdfd.823003.
EndNote Kaya B (December 1, 2020) Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi. Türk Doğa ve Fen Dergisi 9 2 157–169.
IEEE B. Kaya, “Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi”, TDFD, vol. 9, no. 2, pp. 157–169, 2020, doi: 10.46810/tdfd.823003.
ISNAD Kaya, Bülent. “Alchemilla persica’nın Farklı Polaritedeki çözücülerde Antioksidan, Antimikrobial Ve Antiisitotoksik Etkilerinin Belirlenmesi”. Türk Doğa ve Fen Dergisi 9/2 (December 2020), 157-169. https://doi.org/10.46810/tdfd.823003.
JAMA Kaya B. Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi. TDFD. 2020;9:157–169.
MLA Kaya, Bülent. “Alchemilla persica’nın Farklı Polaritedeki çözücülerde Antioksidan, Antimikrobial Ve Antiisitotoksik Etkilerinin Belirlenmesi”. Türk Doğa Ve Fen Dergisi, vol. 9, no. 2, 2020, pp. 157-69, doi:10.46810/tdfd.823003.
Vancouver Kaya B. Alchemilla persica’nın farklı polaritedeki çözücülerde antioksidan, antimikrobial ve antiisitotoksik etkilerinin belirlenmesi. TDFD. 2020;9(2):157-69.