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Isolation and Molecular Characterization Of Antimicrobial Agent Producing Bacteria Isolated From Palandoken Mountain

Year 2021, , 254 - 258, 25.06.2021
https://doi.org/10.46810/tdfd.881932

Abstract

Streptomyces is a genus of Gram-positive bacteria that grows in different habitats, and its shape takes after filamentous fungi. The most effective characteristic of Streptomyces is the ability to produce secondary metabolites, such as antivirals, antifungals, anti-hypertensives, antitumorals, and especially antibiotics. In this study, bacteria producing antimicrobial substances were isolated from soil samples collected from Palandöken Mountain. Test strains were identified using conventional (morphological, physiological and biochemical tests) and molecular methods (16S rRNA sequencing). Then, the antagonistic effect of these bacteria against pathogenic microorganisms was determined by disc diffusion method. As a result of analysis, it was found that two bacteria (AO1 and AO3) were similar to Streptomyces violaceochromogenes, one (AO2) to Streptomyces ambofaciens and other (AO4) to Sphingomonas melonis at a rate of 99%. According to conventional tests, all isolates were catalase positive, three were oxidase negative (except AO4). In addition, pH, NaCl and temperature values that isolates can growth were determined. Finally, phylogenetic trees of the isolates were performed by the neighbor-joining method.

References

  • 1. Awais M, Shah AA, Hameed A, Hasan F. Isolation, identification and optimization of Bacitracin produced by Bacillus sp. Pakistan Journal of Botany. 2007;39(4):1303-12.
  • 2. Burnham CAD, Leeds J, Nordmann P, O'Grady J, Patel J. Diagnosing antimicrobial resistance. Nat Rev Microbiol. 2017;15(11):697-703.
  • 3. Yoneyama H, Katsumata R. Antibiotic resistance in bacteria and its future for novel antibiotic development. Biosci Biotech Bioch. 2006;70(5):1060-75.
  • 4. Martinez JL. General principles of antibiotic resistance in bacteria. Drug Discov Today Technol. 2014;11:33-9.
  • 5. Livermore DM, Antimicrobial BS. Discovery research: the scientific challenge of finding new antibiotics. J Antimicrob Chemoth. 2011;66(9):1941-4.
  • 6. Gould IM, Bal AM. New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013;4(2):185-91.
  • 7. Gupta A, Mumtaz S, Li CH, Hussain I, Rotello VM. Combatting antibiotic-resistant bacteria using nanomaterials. Chem Soc Rev. 2019;48(2):415-27.
  • 8. Overbye KM, Barrett JF. Antibiotics: where did we go wrong. Drug Discov Today. 2005;10(1):45-52.
  • 9. Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing New Antimicrobial Therapies: Are Synergistic Combinations of Plant Extracts/Compounds with Conventional Antibiotics the Solution? Pharmacogn Rev. 2017;11(22):57-72.
  • 10. Vitorino LC, Bessa LA. Microbial Diversity: The Gap between the Estimated and the Known. Diversity-Basel. 2018;10(2).
  • 11. Baltaci MO, Adiguzel A. Isolation, Identification and Molecular Characterization of Cellulolytic Bacteria from Rumen Samples collected from Erzurum Slaughter House, Turkey. Res J Biotechnol. 2016;11(2):32-8.
  • 12. Adiguzel G, Faiz O, Sisecioglu M, Sari B, Baltaci O, Akbulut S, et al. A novel endo-beta-1,4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices. Int J Biol Macromol. 2019;129:571-8.
  • 13. Baltaci MO, Ay H, Akbulut S, Adiguzel G, Albayrak S, Omeroglu MA, et al. Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey. Int J Syst Evol Micr. 2020;70(6):3865-71.
  • 14. Baltaci MO, Genc B, Arslan S, Adiguzel G, Adiguzel A. Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production. Geomicrobiol J. 2017;34(1):53-62.
  • 15. Iglesias MB, Abadias M, Anguera M, Sabata J, Vinas I. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. Lwt-Food Sci Technol. 2017;81:243-9.
  • 16. Fields FR, Lee SW, McConnell MJ. Using bacterial genomes and essential genes for the development of new antibiotics. Biochem Pharmacol. 2017;134:74-86.
  • 17. Van der Meij A, Worsley SF, Hutchings MI, van Wezel GP. Chemical ecology of antibiotic production by actinomycetes. Fems Microbiol Rev. 2017;41(3):392-416.
  • 18. Quinn GA, Banat AM, Abdelhameed AM, Banat IM. Streptomyces from traditional medicine: sources of new innovations in antibiotic discovery. Journal of Medical Microbiology. 2020;69(8):1040-8.
  • 19. Waksman SA, Schatz A, Reynolds DM. PRODUCTION OF ANTIBIOTIC SUBSTANCES BY ACTINOMYCETES (Reprinted from. Ann Ny Acad Sci. 2010;1213:112-24.
  • 20. Sarmiento-Vizcaino A, Espadas J, Martin J, Brana AF, Reyes F, Garcia LA, et al. Atmospheric Precipitations, Hailstone and Rainwater, as a Novel Source of Streptomyces Producing Bioactive Natural Products. Front Microbiol. 2018;9.
  • 21. Zheng B, Han XX, Xia ZF, Wan CX, Zhang LL. Streptomyces lopnurensis sp nov., an actinomycete isolated from soil. Int J Syst Evol Micr. 2014;64:4179-83.
  • 22. Jabila Mary TR, Kannan RR, Muthamil Iniyan A, Carlton Ranjith WA, Nandhagopal S, Vishwakarma V, et al. beta-lactamase inhibitory potential of kalafungin from marine Streptomyces in Staphylococcus aureus infected zebrafish. Microbiol Res. 2021;244:126666.
  • 23. Balasubramanian S, Othman EM, Kampik D, Stopper H, Hentschel U, Ziebuhr W, et al. Marine Sponge-Derived Streptomyces sp. SBT343 Extract Inhibits Staphylococcal Biofilm Formation. Front Microbiol. 2017;8:236.
  • 24. Hong HJ, Hutchings MI, Hill LM, Buttner MJ. The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor. J Biol Chem. 2005;280(13):13055-61.
  • 25. Allen AD, Daley P, Ayorinde FO, Gugssa A, Anderson WA, Eribo BE. Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties. World J Microb Biot. 2012;28(9):2791-800.
  • 26. Kim YG, Lee JH, Kim CJ, Lee JC, Ju YJ, Cho MH, et al. Antibiofilm activity of Streptomyces sp. BFI 230 and Kribbella sp BFI 1562 against Pseudomonas aeruginosa. Appl Microbiol Biot. 2012;96(6):1607-17.

Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu

Year 2021, , 254 - 258, 25.06.2021
https://doi.org/10.46810/tdfd.881932

Abstract

Streptomycesler, farklı habitatlarda gelişen bir Gram-pozitif bakteri cinsi olup, şekli ipliksi mantarlara benzer. Streptomyces'in en etkili özelliği antiviraller, antifungaller, anti-hipertansifler, antitümoraller ve özellikle antibiyotikler gibi sekonder metabolitler üretebilmesidir. Bu çalışmada, test suşları, geleneksel (morfolojik, fizyolojik ve biyokimyasal testler) ve moleküler yöntemler (16S rRNA sekanslama) kullanılarak tanımlandı. Daha sonra bu bakterilerin patojen mikroorganizmalara karşı antagonistik etkisi disk difüzyon yöntemi ile belirlendi. Analiz sonucunda, izole edilen dört bakteriden, ikisinin(AO1 ve AO3) Streptomyces violaceochromogenes'e, birinin (AO2) Streptomyces ambofaciens'e ve diğerinin ise (AO4) Sphingomonas melonis'e% 99 oranında benzer olduğu bulundu. Geleneksel testlere göre, tüm izolatlar katalaz pozitif, üçü oksidaz negatiftir (AO4 hariç). Ayrıca izolatların büyüyebileceği pH, NaCl ve sıcaklık değerleri belirlendi. Son olarak izolatların filogenetik ağaçları komşu birleştirme yöntemi ile yapılmıştır.

References

  • 1. Awais M, Shah AA, Hameed A, Hasan F. Isolation, identification and optimization of Bacitracin produced by Bacillus sp. Pakistan Journal of Botany. 2007;39(4):1303-12.
  • 2. Burnham CAD, Leeds J, Nordmann P, O'Grady J, Patel J. Diagnosing antimicrobial resistance. Nat Rev Microbiol. 2017;15(11):697-703.
  • 3. Yoneyama H, Katsumata R. Antibiotic resistance in bacteria and its future for novel antibiotic development. Biosci Biotech Bioch. 2006;70(5):1060-75.
  • 4. Martinez JL. General principles of antibiotic resistance in bacteria. Drug Discov Today Technol. 2014;11:33-9.
  • 5. Livermore DM, Antimicrobial BS. Discovery research: the scientific challenge of finding new antibiotics. J Antimicrob Chemoth. 2011;66(9):1941-4.
  • 6. Gould IM, Bal AM. New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013;4(2):185-91.
  • 7. Gupta A, Mumtaz S, Li CH, Hussain I, Rotello VM. Combatting antibiotic-resistant bacteria using nanomaterials. Chem Soc Rev. 2019;48(2):415-27.
  • 8. Overbye KM, Barrett JF. Antibiotics: where did we go wrong. Drug Discov Today. 2005;10(1):45-52.
  • 9. Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing New Antimicrobial Therapies: Are Synergistic Combinations of Plant Extracts/Compounds with Conventional Antibiotics the Solution? Pharmacogn Rev. 2017;11(22):57-72.
  • 10. Vitorino LC, Bessa LA. Microbial Diversity: The Gap between the Estimated and the Known. Diversity-Basel. 2018;10(2).
  • 11. Baltaci MO, Adiguzel A. Isolation, Identification and Molecular Characterization of Cellulolytic Bacteria from Rumen Samples collected from Erzurum Slaughter House, Turkey. Res J Biotechnol. 2016;11(2):32-8.
  • 12. Adiguzel G, Faiz O, Sisecioglu M, Sari B, Baltaci O, Akbulut S, et al. A novel endo-beta-1,4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices. Int J Biol Macromol. 2019;129:571-8.
  • 13. Baltaci MO, Ay H, Akbulut S, Adiguzel G, Albayrak S, Omeroglu MA, et al. Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey. Int J Syst Evol Micr. 2020;70(6):3865-71.
  • 14. Baltaci MO, Genc B, Arslan S, Adiguzel G, Adiguzel A. Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production. Geomicrobiol J. 2017;34(1):53-62.
  • 15. Iglesias MB, Abadias M, Anguera M, Sabata J, Vinas I. Antagonistic effect of probiotic bacteria against foodborne pathogens on fresh-cut pear. Lwt-Food Sci Technol. 2017;81:243-9.
  • 16. Fields FR, Lee SW, McConnell MJ. Using bacterial genomes and essential genes for the development of new antibiotics. Biochem Pharmacol. 2017;134:74-86.
  • 17. Van der Meij A, Worsley SF, Hutchings MI, van Wezel GP. Chemical ecology of antibiotic production by actinomycetes. Fems Microbiol Rev. 2017;41(3):392-416.
  • 18. Quinn GA, Banat AM, Abdelhameed AM, Banat IM. Streptomyces from traditional medicine: sources of new innovations in antibiotic discovery. Journal of Medical Microbiology. 2020;69(8):1040-8.
  • 19. Waksman SA, Schatz A, Reynolds DM. PRODUCTION OF ANTIBIOTIC SUBSTANCES BY ACTINOMYCETES (Reprinted from. Ann Ny Acad Sci. 2010;1213:112-24.
  • 20. Sarmiento-Vizcaino A, Espadas J, Martin J, Brana AF, Reyes F, Garcia LA, et al. Atmospheric Precipitations, Hailstone and Rainwater, as a Novel Source of Streptomyces Producing Bioactive Natural Products. Front Microbiol. 2018;9.
  • 21. Zheng B, Han XX, Xia ZF, Wan CX, Zhang LL. Streptomyces lopnurensis sp nov., an actinomycete isolated from soil. Int J Syst Evol Micr. 2014;64:4179-83.
  • 22. Jabila Mary TR, Kannan RR, Muthamil Iniyan A, Carlton Ranjith WA, Nandhagopal S, Vishwakarma V, et al. beta-lactamase inhibitory potential of kalafungin from marine Streptomyces in Staphylococcus aureus infected zebrafish. Microbiol Res. 2021;244:126666.
  • 23. Balasubramanian S, Othman EM, Kampik D, Stopper H, Hentschel U, Ziebuhr W, et al. Marine Sponge-Derived Streptomyces sp. SBT343 Extract Inhibits Staphylococcal Biofilm Formation. Front Microbiol. 2017;8:236.
  • 24. Hong HJ, Hutchings MI, Hill LM, Buttner MJ. The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor. J Biol Chem. 2005;280(13):13055-61.
  • 25. Allen AD, Daley P, Ayorinde FO, Gugssa A, Anderson WA, Eribo BE. Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp JM3 and the evaluation of their antimicrobial properties. World J Microb Biot. 2012;28(9):2791-800.
  • 26. Kim YG, Lee JH, Kim CJ, Lee JC, Ju YJ, Cho MH, et al. Antibiofilm activity of Streptomyces sp. BFI 230 and Kribbella sp BFI 1562 against Pseudomonas aeruginosa. Appl Microbiol Biot. 2012;96(6):1607-17.
There are 26 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Mustafa Özkan Baltacı 0000-0003-4968-9016

Publication Date June 25, 2021
Published in Issue Year 2021

Cite

APA Baltacı, M. Ö. (2021). Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu. Türk Doğa Ve Fen Dergisi, 10(1), 254-258. https://doi.org/10.46810/tdfd.881932
AMA Baltacı MÖ. Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu. TDFD. June 2021;10(1):254-258. doi:10.46810/tdfd.881932
Chicago Baltacı, Mustafa Özkan. “Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu”. Türk Doğa Ve Fen Dergisi 10, no. 1 (June 2021): 254-58. https://doi.org/10.46810/tdfd.881932.
EndNote Baltacı MÖ (June 1, 2021) Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu. Türk Doğa ve Fen Dergisi 10 1 254–258.
IEEE M. Ö. Baltacı, “Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu”, TDFD, vol. 10, no. 1, pp. 254–258, 2021, doi: 10.46810/tdfd.881932.
ISNAD Baltacı, Mustafa Özkan. “Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu”. Türk Doğa ve Fen Dergisi 10/1 (June 2021), 254-258. https://doi.org/10.46810/tdfd.881932.
JAMA Baltacı MÖ. Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu. TDFD. 2021;10:254–258.
MLA Baltacı, Mustafa Özkan. “Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu”. Türk Doğa Ve Fen Dergisi, vol. 10, no. 1, 2021, pp. 254-8, doi:10.46810/tdfd.881932.
Vancouver Baltacı MÖ. Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu. TDFD. 2021;10(1):254-8.