Ağrı İlinde Yetiştirilen Buğday, Arpa ve Yoncanın Rhizosferik PGPR Suşları

Abstract

Mevcut çalışmamızda; Ağrı ili ve çevresinde yer alan tarım alanlarında yetişmekte olan buğday, arpa ve yonca gibi zirai mahsullerden potansiyel bitki büyümesini teşvik eden rizobakterilerin (PGPR) izolasyonu ve karakterizasyonu amaçlanmıştır. Bu amaçla, Ağrı ilinin 12 farklı noktasından buğday, arpa ve yonca rizosferik toprak örnekleri toplanmıştır. Bakteri izolasyonu çalışmaları bu rizosferik örneklerle gerçekleştirilmiştir. Daha sonra her bir izolatın PGPR özelliklerini belirlemeye yönelik; azot bağlama, fosfat çözme, siderofor, amonyak ve HCN üretimi testleri yapılmıştır. Biyogübre, biyostimülan ve/veya biyo-koruyucuların geliştirilmesine uygun olduğu belirlenen aktif izolatların moleküler tanımlaması, evrensel 16S rRNA primerlerinin kullanıldığı PCR ve dizileme uygulamaları ile yapılmıştır. Elde edilen Sonuçlara göre 29 potansiyel PGPR izolatı tanılanmış ve moleküler karakterizasyonu yapılmıştır. Bu izolatlar, 16S rRNA sekans benzerliklerine göre Arthrobacter, Bacillus, Brevibacterium, Enterobacter, Pantoea, Pseudomonas, Microbacterium ve Stenotrophomonas cinsleri içerisinde dağılım göstermiştir.

Keywords

• PGPR
• Azot fiksasyonu
• Fosfat çözünmesi
• Siderofor üretimi
• 16S rRNA

Rhizospheric PGPR Strains of Wheat, Barley and Trefoil Grown in Ağrı Province

Abstract

The present study aimed to isolate potential plant growth rhizobacteria (PGPR) from various crops in agricultural areas in Ağrı-Turkey and their characterization. For this goal, rhizospheric soil samples of wheat, barley and trefoil were collected from 12 different locations in Ağrı province. Bacteria isolation studies were carried out with these rhizospheric samples. Then, to determine the PGPR properties of each isolate; nitrogen fixation, phosphate dissolution, siderophore, ammonia and HCN production tests were performed. Molecular identification of active isolates determined as suitable for development of biofertilizers, biostimulants and/or bioprotectants was done by PCR and sequencing applications performed with universal 16S rRNA primers. According to the results, 29 potential PGPR isolates were determined and their molecular characterization was done. These isolates were distributed in Arthrobacter, Bacillus, Brevibacterium, Enterobacter, Pantoea, Pseudomonas, Microbacterium and Stenotrophomonas according to their 16S rRNA sequence similarities.

Keywords

• PGPR
• Nitrogen fixation
• Phosphate-solubilization
• Siderophore production
• 16S rRNA

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