Bortezomib ile Kalp Hasarı Oluşturulan Ratlarda Berberinin Oksidatif ve Nitrozatif Stres Üzerine Etkisi

Year 2020, Volume: 9 Issue: 2, 118 - 126, 30.12.2020

Cihan Gür , Fatih Kandemir , Aydın Genç

https://doi.org/10.46810/tdfd.809220

Cited By: 7

Abstract

Bortezomib (BTZ), proteazom sistemini bloke ederek hücresel protein yıkımını engelleyen yeni nesil bir antineoplastik ilaçtır. Bu çalışmada BTZ kaynaklı kalp hasarına karşı bir izokinolin alkaloid olan berberinin (BBR) koruyucu etkileri araştırılmıştır. Çalışmada erkek Sprague Dawley cinsi ratlara 1., 3., 5. ve 7. günlerde periton içi 0,2 mg kg-1 BTZ ve 10 gün boyunca her gün 50 ve 100 mg kg-1 dozlarda BBR verildi. Ratların kalp dokularında malondialdehit (MDA), glutatyon (GSH), total antioksidan kapasite (TAK), total oksidan kapasite (TOK) ve nitrik oksit (NO) seviyeleri ile süperoksit dismutaz (SOD), katalaz (KAT), glutatyon peroksidaz (GPx) ve laktat dehidrogenaz (LDH) aktiviteleri biyokimyasal yöntemler ile analiz edildi. Veriler BTZ’nin kalp dokusunda MDA, NO ve TOK seviyelerini, serumda LDH aktivelerini ve oksidatif stres indeksini (OSI) önemli ölçüde arttırdığını, GSH ve TAK seviyeleri ile SOD, KAT ve GPx aktivitelerini azaltarak oksidatif stresi tetiklediğini buna bağlı olarak dokuda hasar oluşturduğunu gösterdi. Buna karşın BBR, BTZ’nin neden olduğu oksidatif stresi ve nitrozatif stresi hafifleterek kalp hasarına karşı koruyucu etki gösterdi. Sonuçlara göre BBR’nin, BTZ ile indüklenen kardiyak toksisiteye karşı daha ileri çalışmalardan sonra koruyucu olarak kullanılabileceğini gösterdi.

Keywords

Berberin, Bortezomib, Kalp, Nitrozatif Stres, Oksidatif Stres

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